Research And Application Of Capture And Ligation Probe Pcr

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Purpose The past decade has seen programs established for malaria controls achieve impressive success. However, the need for sensitive and cheap methods for active screening of malaria parasites in non-endemic settings is still urgent. With the prevalence of the malaria parasite approaching elimination, there is the need for an increase in case detection just to identify a single positive infection. This increase is an expensive venture especially for malaria control programs, and there is a need to develop an assay that is affordable while maintaining high sensitivity and achieving high throughput. The researchers describe the development and application of Capture and Ligation Probe-PCR (CLIP-PCR) to effectively counter these challenges.…show more content…
There is a need for diagnostic techniques like PCR with high sensitivity to provide accurate malaria diagnosis. With the active elimination of malaria infection, experts must conduct more case detections to identify one positive infection case (Bronzan, McMorrow & Kachur, 2008). The use of PCR may be limited because of its high cost per test (Cheng et al., 2015). Additionally, the use of active screening that is free of charge may encourage community-wide participation the (World Health Organization [WHO], 2012). The increase in massive screening is already straining the financial resources of many malaria control program. The need for technology advancement to produce screening methods with higher throughput at a lower cost and higher sensitivity is apparent. This work describes the development of Capture and Ligation Probe-PCR (CLIP-PCR) to meet these challenges. ). Reverse-transcription sensitivity PCR was achieved but got rid of its reliance to RNA reverse transcription and purification. Laboratory prepared samples used on a broad range of parasite pool sizes and densities before being applied to active screened dried blood spot samples gathered from 3 areas in China which are endemic. 18S rRNA of a genus Plasmodium is drawn from the blood and captured onto 96-well plates (Cheng et al., 2013). In addition to this, the 18S rRNA is measured by the amount of ligated probes that bind continually to it. ResultInteresting aspect Cheng et al. (2015)

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