Research And Application Of Capture And Ligation Probe Pcr

Malaria infection is caused by parasitic protozoan plasmodium. Several species of malaria parasites exist but the most fatal known is plasmodium falciparum. It enters the liver and replicates itself by getting into the red blood cell and ripping its way out when fully matured. It was once thought that the US military got close to completely eradicating the vector mosquito through DDT (dichlorodiphenyltrichloroethane) and the parasitic plasmodium with vaccines until they discovered that both are capable of mutating and gained the ability to be resistant to any chemicals scientists could produce (Horizon, 2005).

Malaria has been a major life-threatening disease for thousands of years, and continues to threaten millions of lives around the world. It infects approximately 219 million people each year, mostly poor women and children. What is striking about malaria is the fact that it has the worse effect on those with the least ability to fight the disease. The Republic of Cuba believes that malaria prevention, treatment, and research efforts must be accelerated to eliminate the burden of this disease across Africa, Asia, the Americas, and any country or territory at risk of malaria transmissions.

Brian, G, Y., Greenwood, D, A., Fidock, Dennis, E, K., Stefan, H, I. Kappe, P., Alonso, L., Frank, H and Collins, P (2008) “Malaria progress and prospects for eradication.” Journal Clinical Investiment. 118: 1266-1276.

Malaria is diagnosed in two different ways. The most exact way is by an examination of the blood. To do this, a doctor would take a drop of blood, stain it, and look at it under a microscope to see if there were any parasites in it. Diagnosing malaria by the symptoms it causes is not as exact as blood examination, but is used a lot in Africa, where most cases are treated at

Background - Malaria is a water borne disease. It is spread by a parasite-carrying mosquito. It kills many people and reduces a country 's capacity to develop. There are different strategies to combat malaria. Around half the population is at risk of malaria and this disease is active in 106 counties across Africa, Asian and the Americas (see source 3). the global annual mortality from malaria is between 1.5 - 3 million deaths, or between 4000 and 8000 each day. Developing countries are most vulnerable to Malaria and as shown on source 2 Malaria has been spread across many various other countries including in Europe, but these countries have eradicated Malaria.

What does the PCR do? PCR is a method by which fragments of DNA can be duplicated. This makes PCR more sufficient amongst other Felds in forensic science.

Malaria has been a huge problem among many developing nations over the past century. The amount of people in the entire world that die from malaria each year is between 700,000 and 2.7 million. 75% of these deaths are African children (Med. Letter on CDC & FDA, 2001). 90% of the malaria cases in the world are located in Sub-Saharan Africa. Once again, the majority of these deaths are of children (Randerson, 2002). The numbers speak for themselves. Malaria is a huge problem and needs to be dealt with immediately.

Prompt and accurate diagnosis is critical to the effective management of malaria. The global impact of malaria has spurred interest in developing effective diagnostic strategies not only for resource-limited areas where malaria is a substantial burden on society, but also in developed countries, where malaria diagnostic expertise is often lacking [4,5]. Malaria diagnosis involves identifying malaria parasites or antigens/products in patient blood. Although this may seem simple, the diagnostic efficacy is subject to many factors. The different forms of the 5 malaria species; the different stages of erythrocytic schizogony, the endemicity of different species, the interrelation between levels of transmission, population movement, parasitemia,

(Fairhurst RM, Wellems TE. Plasmodium species (Malaria). In: Mandell GL, Bennett JE, Dolin R, eds. Principles and Practice of Infectious Diseases . 7th ed. Philadelphia, Pa: Elsevier Churchill Livingstone; 2009: p. 3071-3072)

Malaria is a vector borne infectious disease which contains 5 subspecies, of which Plasmodium ovale is the most likely culprit in this case study. The species of malaria can be distinguished between by thin blood films and analysing haematological abnormalities present, such as in plasmodium falciparum infection no change occurs to erythrocyte shape and size whereas in P. ovale infection erythrocytes are usually slightly enlarged, frequently an oval shape and often have fimbriated edges (Moore, Knight and Blann, 2016).

PCR is an efficient molecular genetic diagnostic process. However, there are 4 main reasons that can make this diagnosis failing to give a precise result, which are the carry of PCR inhibitors in sample, the lack of optimization of the PCR primer and probe design, the lack of investigation in standard curve and the inaccurate sample and reagent pipetting (reference).

Loop-mediated isothermal amplification (LAMP) and reverse transcription (RT)-LAMP have been used to detect several types of infectious reagents with high sensitivity, efficiency, and rapidity under isothermal conditions (5, 8, 9). The need for simple, visible, and rapid detection of LAMP/or RT-LAMP amplicons has led to a great variety of end point and real-time/on-line monitoring for field applications (10, 11). According to the general principles of LAMP amplification, 4 to 6 primers are designed to react to specific regions on the target sequences. When appropriate initiating structure

About 3.3 billion people, that is about half of the world’s population are at risk of contracting malaria (figure 1). Every year there are 250 million cases of malaria, and nearly 1 million deaths. That amounts to 2,732 deaths per day. Out of those million people that die every year, 800,000 of them are African children under the age of 5. To control malaria three actions need to be taken: insecticides need to be used to decrease the vector population, people have to be educated as to how to prevent the vector from reproducing, and anti-malarial drugs need to be distributed. To understand the vector and what the vector is, scientists had to first discover what the parasite was and how it worked. It was not until the year 1880 that French Physician Charles Laveran discovered that Malaria was caused by a protozoan in the genus Plasmodium (Malaria, 2013)

(pLDH) in diagnosing malaria using microscopy and molecular test(PCR) to see if there is association between the deletions of the histidinerich central repeat region of the hrp2 gene (exon 2) and false-negative

Introduction: Worldwide emergence and spread of antimalarial resistance has led to use of molecular markers in monitoring of resistant plasmodium parasite. Single nucleotide polymorphisms in the K13 propellor domain have been recently associated with artemisinin resistance. This study aims at profiling K13 molecular markers related to reduced susceptibility to Artemether-Lumefantrine in parasite isolates from Msambweni coastal region, Kenya. Methods: Chelex method was used for extracting DNA from Plasmodium falciparum positive dried blood spots on filter paper samples. Polymerase chain reaction and sequencing technique was used to detect single nucleotide polymorphism in K13 propeller gene. MEGA 6 was used to identify specific SNP combinations using wild-type reference sequence (XM_001350122).