Essay about Restriction Enzyme Lab Report

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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday, 1:10 pm Date of Experiment: October 25, 2012 III. Introduction. Restriction enzymes (or restriction endonucleases), originally isolated from Haemophilus influenzae in 1970, are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction sites generally are four to eight base pairs in length. It is thought that, together with enzymes that methylate portions of native DNA, restriction enzymes protect cells from DNA of invading organisms cutting such DNA into pieces, thereby…show more content…
Graph I (Standard Curve). As discussed in the introduction above, the distance traveled by a DNA fragment toward the anode in agarose gel electrophoresis has a linear relationship with the log of its length (in base pairs). The graph attached as Exhibit B is a scatter plot of the data from the final two columns of Table 1 above, with a trendline showing the relationship between the variables. Note that data for fragment greater than 5,000 base pairs in length are shown on the plot but were not used in calculation of the trendline as a 1% agarose gel was used during electrophoresis and such a gel concentration does not effectively resolve fragments of lengths greater than 5,000 base pairs. 4. Table II. The following table presents the data obtained by performing electrophoresis on the DNA fragments from the pBR322 restriction enzyme digests. |Band # |Distance
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