Results And Discussion Of Pcr And Dsrna Production Essay

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RESULTS AND DISCUSSION PCR and dsRNA production Target sequences of 1501, 1576, 1650, 1750, 538, and 716 bp specific for gyrase A, gyrase rpo B1, rpo B2 and GFP were successfully amplified with PCR (data not shown).. The in vitro transcription using T7 enzyme resulted in target specific dsRNA of 1501, 1576, 1650, 1750, 538, and 716 bp for each of gyrase A, gyrase B, rpo B1, rpo B2, L11, and GFP genes, respectively (Fig.1). In vitro growth inhibition assay B. bovis growth (Fig. 2) from an initial parasitemia of 1% was significantly (ANOVA) inhibited at 10 µg/ml and 50 µg/ml concentrations of gyrase A, gyrase B, rpo B1 and B2, and L11 (Fig. 2). Treatment with a mixture formed of 10 µg/ml gyrase A and 10 µg/ml gyrase B significantly inhibited the growth (ANOVA, P < 0.01). The mixture formed of 10 µg/ml rpo B1 and 10 µg/ml rpo B2 resulted in significant inhibition of the growth (ANOVA, P < 0.01) (Fig. 2). The single treatment with 10 µg/ml or 50 µg/ml of each of the dsRNA of gyrase A, gyrase B, rpo B1, rpo B2, and L11 (Tukey-Kramer HSD) did not show significant difference. There was no significant difference between the single treatment with 10 µg/ml or 50 µg/ml of each dsRNA and the mixture formed of 10 µg/ml gyrase A and 10 µg/ml gyrase B dsRNAs and the mixture formed of 10 µg/ml rpo B1 and 10 µg/ml rpo B2 dsRNAs

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