Results of Drug Testing on Rats

3306 Words Feb 18th, 2018 13 Pages
Ethanol was obtained from PureScience (Wellington, NZ).
Cocaine-HCl (BDG Synthesis, Wellington, NZ) was dissolved in physiological saline (0.9%) containing heparin sodium (3 U/mL) and made up to a solution of 1.65 g/L (rats were considered to average 330 g). Each intravenous infusion was 0.1 mL and delivered over a period of 12 s (0.5 mg/kg/infusion). For intraperitoneal injections, cocaine-HCl was dissolved in physiological saline to a concentration of 2 mg/mL.
Surgery equipment: silastic and tygon tubing were from Thermo Fisher Scientific (Auckland, NZ), silicon, cotton buds and Bostik superglue were obtained from the Warehouse (Wellington, NZ), while gauze swabs were purchased from Global Science (Auckland, NZ). Large screws (CS00395) were obtained from Coastal Fasteners (Wellington, NZ) and small screws (MCPF40210) from Eyeline Optical (Wellington, NZ). The Ostron powder and liquid were obtained from Henry Schein Shalfoon (Auckland, NZ).
Syringes and needles were acquired from Becton Dickinson (Auckland, NZ) while the minisart filters (0.2 µm) were from MicroNZ.

2.1.2 Subjects
Male B6-SJL mice were obtained from the Malaghan Institute of Medical Research (Wellington, NZ) for tail withdrawal assays while male Sprague-Dawley…

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