Review of Research Paper on Metastasis of Cancerous Tumors

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Introduction Early diagnosis provides the best hope many have in beating cancer. Unfortunately, this is not the end of the fight for many cancer patients. Primary tumors may be successfully treated early, only to later discover they have metastasized to other areas of the body. The common medical definition of metastasis is: The spread of cancer from one part of the body to another. Tumors formed from cells that have spread are called “secondary tumors” and contain cells that are like those in the original (primary) tumor. This definition could be the reason why early treatment of a primary tumor is ineffective against metastatic tumor cells. As the definition suggests, the accepted model…show more content…
Of these test were the PCR, CGH, LOH, and genomic loci probability evaluation test. From these experiments, a conclusion was then formed about whether cancer cells differentiated before metastasis. Listed below are the procedures in detail. These are the procedures/ techniques performed: *Tumor Cell Detection and Sample Preparation: -386 breast cancer patients had bone marrow aspirated. :Of patients with no signs of metastasis who had samples drawn: -44% drawn before surgery -39% in next month -17% 1month to 10 years after surgery of primary tumor -Bone marrow suspensions. -Staining, tumor cell isolation, comparative genomic hybridization -Micro dissection of primary tumor, tissue preparation -PCR and CGH of isolated DNA Samples were drawn to perform the PCR and CGH tests for isolated DNA: *Loss of Heterozygosity Analysis(LOH): -Analysis of primary PCR and micro dissected tumors and single cells -Addition of microsatellite markers and primers to DNA -Scanning of Polyacrylamide gels by FluorImager *BioInformatic Evaluation: -46 genomic loci features selected and ranked according to information status -defined the presence of clinically evident metastatic targets -built classifier that assigns each cell its probability of being isolated from M1 patients, based on clinical variables of the primary tumor and genomic loci of isolated tumor cell. :Genomic Loci were selected to identify and differentiate cells. By

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