Rna Interference ( Rnai ) Drugs

1382 Words Dec 7th, 2014 6 Pages
RNA interference (RNAi) drugs are drugs that alter the protein production of the desired cells/tissues by altering their RNA. The field of RNA interference is fairly recent with study of the mechanisms that enable it starting in the 1990s and continuing today. Through the years, it has been known as co-suppression, post transcriptional gene silencing, quelling, and others due to the ability to deactivate or suppress certain genes, mostly in Eukaryotes. RNAi has proved to be a very powerful instrument in medicine, biotechnology, genomics and others. The medicines currently produced can actually stop the production of certain proteins to treat specific illnesses, essentially making a genetic “off switch” for certain genes. A common focus of …show more content…
Through this, they sought to understand why 30 nucleotide strands of RNA were under processed while 21-22 nucleotide stands are more efficiently processed and better used as mediators and how sense and antisense are affected by the dsRNA processing direction. They detected the 21-23nt fragments suspected to be produced by the dsRNAs. The conclusion from this, combined with previous experimental data, is that 21-23-nt fragments help guide mRNA cleavage, explaining the lack of 30 base pair dsRNAs. This reveals that the natural mechanism likely does this in order to prevent undesired RNAi by the shorter intramolecular cellular RNAs, a type of natural security system. Through the mapping processes they undergo from their in vitro tests and connecting the results of others to their own, they provide support for the most updated model for RNA interference and mRNA cleavage. In the article by Wizenerowicz and Trono, they explore how Drug-Inducible RNA Interference suppresses genes with respect to Lentivirus vetors. They seek to expand the possibilities of RNA Interference by better contolling conditional suppression of genes in mammals. They achieved efficient and drug-inducible suppression (using a lentivirus vector background) on promoter dependent polymerase III production through the use of doxycycline-controlled transcriptional repression. They attempted
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