Rules And Procedure (P1)

HYDROCHLORIC ACID IS A CORROSIVE SUBSTANCE WHICH CAN CAUSE DAMAGE TO SKIN AND OTHER SUBSTANCES. IT HAS STRONG VAPORS. WEAR GOGGLES AND AN APRON. YOUR TEACHER WILL SHOW YOU HOW TO POUR THE ACID SAFELY. IF YOU SPILL ACID ON YOURSELF, WASH IT OFF WITH LOTS OF RUNNING WATER. A FEW DROPS SPILLED ON THE LAB BENCH CAN BE CLEANED UP WITH A PAPER TOWEL. A LARGE SPILL SHOULD BE NEUTRALIZED WITH BAKING SODA, AND THE RESULTING NEUTRAL SUBSTANCE CAN THEN BE WASHED INTO THE SINK WITH A

Hydrochloric acid (HCl) has risks as it is a corrosive compound. Corrosive compounds, including HCl, can cause injuries such as burning and eating away skin and eyes. To prevent such injuries, do not come into physical contact with HCl. Wear safety glasses, safety gloves and lab clothing.

The two major safety issues are proper disposal of the glass strands created by cutting and trimming the fiber or accidentally breaking it, and the cleaning chemicals and adhesives used in installations.

Hand protection; wearing gloves, if you ever handle chemical it will protect your hands if it spills

When handling the Acid or Base to avoid getting any substance in eyes: Use protective eyewear

Prescription safety glasses are a shield that protect your eyes from hazardous work and home environments. The most common hazard is flying projectiles from operating power tools or machinery. Others include harmful dust, splashing chemicals, laser light, and radiation. However, a poor fit compromises your eye safety in a number of ways. These include:

Caution – Hydrochloric acid is corrosive to eyes, skin and clothes. Wearing a lab apron, rubber gloves, goggles, and a face shield is essential. Rinse any spills on skin or clothing with plenty of cold water. Clean up spills immediately – ask your instructor for help.

Dr. Spaulding created a classification system to distinguish which items are critical, semi-critical, and non-critical according to the risk of the procedure. Some critical or high-risk items are items that can be inserted into a sterile body cavity and/or tissue during surgery. The probe should be sterilized before the procedure and sterile gel is to be used. A sterile probe cover may also be used. Objects that are used on mucous membranes are considered semi-critical such as a trans-vaginal ultrasound probe. Sterile water may be used when a probe is used in a biopsy procedure. High-level disinfectants are recommended for procedures that are involved within the body. Objects used on intact skin, such as the torso, are considered

Care should be taken when handling beakers, graduated cylinders, and any other glassware. It is also important to make sure beakers and graduated cylinders are not slippery, to prevent accidents. Since hydrochloric acid is harmful, even at low concentrations, glasses must be worn when using and handling it. Crystal violet also stains, so to avoid getting it on skin and clothing, gloves and an apron can be used if desired. Also be careful when pouring or using any of the chemicals, because they can cause irritation and can be harmful to skin and eyes.

Fold and tie the other end of the dialysis tube and cut all extra string as close to the knot as possible. Repeat step 7 with the third dialysis tube, except fill with 20 mL of sugar solution from beaker 1. Do not confuse the dialysis tubing!! Mass all three of the dialysis tubes and record their weight with an electronic balance.

Sunglasses were initially created to diminish disturbing glare and permit more comfortable viewing in bright light (Belikoff). However, the negative effects of the sun were soon discovered and new technology was needed in order to protect our eyes by screening out the sun rays (Belikoff). The purpose of sunglasses is to protect eyes from excessive sunlight (Belikoff). Both visible and nonvisible spectra radiation can damage extremely sensitive eyes (Belikoff). Overexposure to that radiation can cause soreness, headaches, and even damage lens, retina and cornea permanently (Belikoff). In the short term, overexposure can worsen one’s vision, which is sometimes called as snow blindness or welders’ flash (Belikoff). In the long run however, overexposure can cause an entire loss of night vision (Belikoff). All of this is caused by ultraviolet (UV) light, which “literally burns the surface of the cornea. (Belikoff)”

Always be familiar with the hazards of a product before you start using it. You should look at an SDS, match the name of the product on the container to the one on the SDS, know the hazards, understand safe handling and storage instructions, as well as understand what to do in an

Place the tube in the numbered slot inside the micro haematocrit centrifuge and wait for about 50 minutes.

It is one of the most dangerous acid and needs to be treated differently as compared to Hydrochloric or Sulfuric Acid.

Blurred vision and pain if used in excess or higher concentrations are used. Wear protective gloves and eye protection (goggles) must be worn. Do not eat or drink during work. Wash hands and exposed body parts thoroughly with soap or disinfectant and shower when possible. Plenty of water to drink.