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SD Rat Lab Report

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General conditions and observations: During the 1st 24 hours post SAP induction in the SD rats, we registered the general conditions of the SD rats as follow. In the control group (PBS injecting), the rats were totally looked well and no death or any abnormalities. Opposite of the SAP induced group were had different symptoms like piloerection, laziness, depression, lags in responses and breath shortness with 4 deaths of ten (40%) (Table1). Post Qingyi Decoction treated in well inducted ASP SD rats the general observation was improved to look well. Thus, the above-mentioned symptoms were disappeared with only one of ten SD rat’s death (10%) (Table 1) (p.value < 0.0001). As well as, the statistical difference between the morbidity rates …show more content…

1). Opposite to STIM1/Ori1SOCE expressions both in genes and proteins level, indicating that STIM1/Ori1SOCE playing a key role in the protective effect of Qingyi Decoction versus ASP-induced myocardial injury (Fig.2A) (P.value <0.001), (Fig.2B) (P.value <0.001) and (Fig.2C) (P.value 0.001) (Fig. 3a+b) with no statistical difference with 2-APB group (p. value 0.7491) (Table1, Fig.2). Qingyi Decoction treatment improved the immunity in SAP rats The effect of Qingyi Decoction on the pathological scores of the SD rat’s module’s hearts which abled to reduce the level of the cytokines in serum (ELISA) of ASP modulated rats. As shown in (Fig.4), the levels of the immune cytokines (IL-1ß, IL-6, IL-8, IL-12, AMY and TNF-α) in the serum was markedly lower in the QYKL than ASP group (p.value 0.0097, 0.0420, <0.0001, 0.0064, <0.0001 and <0.0001) (Table 2) subsequently with rat’s general conditions (Table 1), abolished the effect of Qingyi Decoction. Table 2. Effect of Qingyi decoction on the serum cytokines mediators level: Group Case(n IL-1β IL-6 IL-8 IL-12 AMY TNF-α Sham 10 09.2±1.8 61.7±4.0 109.8±6.0 062±6.0 076±3.0 121±4.0 SAP 10 17.3±2.2 93.0±5.0 116.8±4.0 099±5.0 154±6.0 354±6.0 2-APB 10 11.0±09 76.0±8.0 128.0±9.0 076±7.0 102±2.0 163±9.0 QYKL 10 11.9±1.7 69.0±6.0 132.0±2.0 073±4.0 114±4.0 161±7.0 Results are expressed as the mean ± standard deviation. aP<0.01, vs. sham group; bP<0.01, vs. SAP group. IL-1ß, IL-6, IL-8, IL-12,

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