Salmonell A Common Bacteria

There are many types of bacteria that can be found in your bathroom. They might also be found on your toothbrush! I have been researching about what bacteria are lurking in my bathroom. I placed 6 petri dishes filled with agar in my bathroom. I left them there for 24 hours to collect bacteria samples.

Often scientists work with bacteria that do not come in a labeled test tube— for example, bacterial samples taken from infected human tissue or from the soil—and the scientist must then identify the unknown microorganism in order to understand what behavior to expect from the organism, for example, a certain type of infection or antibiotic resistance. However, because of the relatively few forms of bacteria compared to animals and because of the lack of bacterial fossil records due to their asexually reproductive nature, the taxonomy used to classify animals cannot be applied to bacteria (Brown 275). In order to classify unknown bacteria, a variety of physiological and metabolic tests are available to narrow a sample down from the fathomless number of possibilities into a more manageable range. Once these tests have been performed, the researcher can consult Bergey’s Manual of Determinative Bacteriology, a systematically arranged and continually updated collection of all known bacteria based on their structure, metabolism, and other attributes.

This lab experiment was done for the purpose of learning how to determine a gram negative bacterium based on multiple tests learned throughout the semester. My gram negative unknown bacterium given to me was Salmonella typhimurium based off of the following tests; Triple Sugar Iron Agar (TSIA), Sulfate Indole Motility (SIM), Methyl Red (MR), Voges-Proskaur (VP), Citrate, Urea Hydrolysis, and Gelatin Hydrolysis. Each test performed gives results such as motility, acid production, fermentation, carbon requirements, or detection of certain coenzymes. With a process of elimination, I determined which bacteria it was not and which bacterium I had, S. typhimurium. The expectation was to master the techniques for each test and utilize the results to determine the unknown bacterium I was given within a two-week period.

The smaller portion contains the ttrRSBCA operon, which is involved in tetrathionate reduction, and seven open reading frames (ORFs) of unknown function which were suggested that these genes do not significantly contribute to systemic infections in mice. The larger portion was characterized to be importance for ability of Salmonella to survive and replicate inside host cells (epithelial cells and macrophages) within the Salmonella-containing vacuole (SCV). The SPI-2 also encoded for T3SS, which encoded for translocon machinery (SseBCD), a pore-forming structure (5, 6).

Gastrointestinal diseases are pervasive problem that is caused by ingestion of contaminated foods or water in which an extensive etiological agents known as enteric pathogen are present, [1]. Salmonella is one of the most opportunistic gram negative, facultative anaerobic food borne pathogens that represent a major health problem and a substantial cause of food poisoning[2]. Salmonellosis caused by Salmonella enterica serovar Typhimurium (S. Typhimurium) stance as the second most prevalent cause of acute gastroenteritis that affecting one million illnesses in the United States, with 19,000 hospitalizations and 380 deaths[3]. Infection begins with the ingestion of contaminated food or water then characterized by attachment of the bacteria by fimbriae or pili to cells lining the

Salmonella enterica: is a member of the Enterobacteriacecae family, a rod shape Gram-negative bacterium. After passage through the gastric acid barrier, S. enterica binds tightly to specific receptors of the epithelial cells in small intestine. The receptor interaction activates a cellular response results in transfer of bacterial effector proteins into the epithelial cell, following by uptake of bacterial cell by endocytosis. Invasion of epithelial cells induce an inflammatory response that increases the fluid secretion of epithelial cells, causing diarrhea. [2]

Background – Microscopic, single celled organisms known as bacteria can be found everywhere. They can be beneficial but they can also be dangerous, such as when they cause infection, which can lead to diseases. Escherichia coli is a germ, or a bacterium, that lives in the digestive paths

The gram-negative bacilli were first discovered and described by Theodor Escherich, a German pediatrician in 1885. Originally named as Bacterium coli commune, the name was changed to Escherichia coli (E.coli) (Stanford T. Shulman et al., 2007). E.coli is known as the most free-living organism being studied. More than 700 E.coli serotypes have been discovered. Their “O” antigen (somatic), and “H” antigen (flagellar) and “K” antigen (capsular) distinguish the different E.coli serotypes. E.coli is commensal bacterial species of the mammalian colon. The bacterium typically colonizes the gastrointestinal tract of an infant within a few hours of life. It is known that E.coli and its human host-derived a mutual benefit that they coexist in good health. Most of E.coli strains are harmless except in immunocompromised hosts, or when gastrointestinal barriers are violated that even non-pathogenic strains can cause disease. Nevertheless, there are certain serotypes that may cause disease in humans and animals. Pathogenic E.coli can be divided into six recognized diarrheagenic categories: enteropathogenic E.coli (EPEC), Shiga toxin-producing E.coli (STEC), enterotoxigenic E.coli (ETEC), enteroaggregative E.coli (EAEC), enteroinvasive E.coli (EIEC) and diffusely adherent E.coli (DAEC) (James B. Kaper et al., 2004). E.coli caused a major health problem in developing countries such as China and Korea. It has been found to be associated with most gastrointestinal diseases in

In this process, I am determined to know my unknown bacteria. So I start by inoculating my unknown bacteria, and spread it on the surface of my EMB medium agar, I take the EMB medium and place it on my incubator chamber. I will get my result on the following day. When the day comes, I walk to the incubator to take out my result, and I found out that it is showing shining green culture on top of the agar. I notice that it is a positive result, and I follow through the dichotomous key, which help me to separate the bacteria from others base on this characteristic. EMB test for Gram-negative bacteria because it has a built-in lactose fermentation that allow them to grow. I make another culture of the unknown bacteria for the following day.

There have been numerous times where I have been affected by Salmonella, so it was easy to swing towards Salmonella as the CBRN choice. Salmonella is found in everyday food products such as raw chicken, raw beef, raw fish, raw eggs and unpasteurized milk (foodsafety.gov). Salmonella can also be found on reptiles, amphibians, birds and even pet treats (foodsafety.gov). People will start to feel symptoms within four to seven days which include: nausea, vomiting, abdominal pain, diarrhea, fever, headaches, dehydration and possibility blood in the stool (mayoclinic.gov).

It is important in the way food is handled and the safety of the process that food goes through. One of the most common causes of food poisoning is bacteria and viruses. Symptoms of food poisoning can range from very sever to not very, depending on what bacteria or virus is the cause. Salmonella is the name for a common group of bacteria. The bacteria can effect different people in different ways. It can cause a serious sickness in older adults, infants, and persons with chronic diseases. Cooking and pasteurization can kill Salmonella. Two caused by this bacterium are the lettuce recall from major supermarkets and the mayonnaise contamination on Melbourne Cup Day, by Piccalilli Catering.

The commercial food industry has a great need in identifying Salmonella to prevent disease in consumers. As well as being the cause of enteric (typhoid) fever, an important infectious disease, Salmonella is perhaps best known as a cause of bacterial food poisoning. Although typhoid fever has been largely eradicated in the developed world, Salmonella food poisoning has long been, and continues to be, an important global public health problem. In much of Europe

Some believe that the media is the enemy of all people. Millions are exposed to media on a daily basis and because we are so gullible and susceptible, we believe everything we see. This can be very problematic because serious events can be looked over by popular headlines such as, “Kim Kardashian has a secret pregnancy!”. However, not all articles are fake. An article about a recent salmonella outbreak, the further findings on sugar and cancer correlation, and an update on Wendy Williams prove that there are real news in the world. There are ways to determine if what you’re reading is real or fake: current date, cited sources and headline presentation.

Picture this, a man is at the grocery store picking up ingredients for a cake. It is his daughter’s seventh birthday and the whole family is going to bake her a cake. He picks up eggs, flour, sugar, and some frosting. After getting home, he and his two other kids are mixing raw eggs into the batter. They all decided to taste it before putting it into the oven. Bam! A week later they all have Salmonella. How did this happen? Well, those eggs that he bought had been produced in a factory farm where animal welfare is not taken into account. They came in contact with feces and dead birds. Does this sound like the kind of food you want to eat? The unjust treatment of animals in the food industry effects not only these animals quality of life, but

Salmonella can backfire or do a lot of bad things in the body in many ways. “Some