Scientific Method Lab Report

When the pH is not at its optimum, the differing pH's will disrupt the bonding between the R groups of the amino acid causing its structure and the shape of the activation site to change

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

f. The student is conducting a nutrition experiment using lab rats. One group (control group) is fed a normal diet, while the other group (experimental group) is fed a diet high in protein. There were 30 rats total used in the experiment. At the end of the experiment, the average mass (weight) of a rat in the control group was 60.4 g while the average mass (weight) of a rat in the experimental group was 0.0689 kg. What is the average difference in weight between the two groups in grams?

Even despite electric shocks, they continued to eat until they became obese. When the researchers switched the obese rats’ diet to a healthy one, the rats refused to eat for almost two weeks. The rats that ate the fast food stopped responding to Leptin, a hormone that regulates hunger. As the rats became more obese, the amount of Leptin in their bodies indicated that the rats were on the brink of starvation; however, the rats continued to eat and become even more obese (Greviskes, 2013).

Statistical analysis for the animal studies. Our selection of 10 animals per time point is based on the group variance observed in our preliminary studies employing diet models. For example, an n = 10 provides 95% power (alpha of 0.05) to detect differences in ALT values of at least 30 U/L between two means with a standard deviation of 10 (two-tailed t-test). For endpoints such as ALT values, caspase positive cells, etc., in different animal populations, parametric analysis will be performed. ANOVA will be employed given the presence of multiple groups, and a Bonferroni test will be used to correct for multiple comparisons. If the distribution of values is unsuitable for a parametric analysis, we will perform a Kruskal-Wallis test for nonparametric data. If the data show a strong trend towards significance (e.g., p < 0.1) with reproducible data except for a rare outlier, we can consider increasing the number of animals per group. The number added will be calculated using the mean and standard deviation (SD) from the existing data

During this week, I Edgar Burgos had to conduct a food intake for 3 consecutive days. This paper will describe my nutritional endeavor for the past three days. I will provide different aspects of my nutritional needs and an in depth analysis on how proteins fats carbohydrates and fiber take part in my everyday diet and what are these functions. This is intended to broaden my views of a healthy lifestyle, and how to achieve it through a variety of food groups.

The prevalence of diseases in the Western world that are affected by our diet have caused us to re-examine what we eat. The discordance hypothesis proposed by Eaton and Konner explains simply that what we are adapted to eat is in discordance with our modern diet. (Eaton & Konner 1985). Comparative studies have been done on primate and human diets to better elucidate the origins of our diet and how modern deviations affect us. In this paper I will examine what we have learned about the evolutionary history of our diet based on studying primates and how this effects our understanding of disease processes caused by our modern, Western diet.

This paper is intended to explain my nutritional endeavor for the past three days. I will be providing different aspects of my nutritional needs and an in depth analysis on how proteins fats carbohydrates and fiber take part in an everyday diet and what are these functions. This is intended to broaden my views of a healthy lifestyle, and how to achieve it through a variety of food groups. In this essay the resources utilized where provided by the educational institute with the purpose of facilitating my research objectives.

The animal experiment was approved by the University of Calgary Animal Care Committee (#AC12–0033). 32 male Sprague Dawley rats were ordered when they were 10 days old (Charles River, Montreal, QC, Canada) and randomized into treatment groups after a two-week adaptation interval. The four treatment groups were as follows (n=8): 1. Control (CON, animals fed ad libitum diet) 2. 75% restriction group (75%, animals fed 75% of the diet consumed by the control animals) 3. 50% restriction group (50%, animals fed 50% of the diet consumed by the control animals) 4. 50% restriction group and then switched to ad libitum diet after two weeks (50% +CON). The rats weighted ~80g when housed individually in metabolic cages of the Comprehensive Lab Animal Monitoring

The subjects were 10 Rattus norvegicus rats obtain from a local breeder and the rats weighed between 200-400g at 12 months old in the beginning of the experiment (cite). Mice were maintained in barrier cages 3 – 4 mice per cage in an environmentally controlled room with filtered air (cite). The laboratory had a set light and a dark light room but majority were set to light phase of 1500- 1800h (cite). The half of mice were on a feeding schedule of intermittent fasting and time restricting fasting, but water was supplied threw out the experiment.

Plasma samples will be collected from mice in each group before feeding the experimental diets and after 10-day feeding period, the length of feeding experimental diets will be based on a previous publication [2].

The overall purpose of the lab is to have the students practice designing an experiment, gathering data, and then analyzing that data to form a conclusion using the scientific method. It also served to understand key terms such as hypothesis, dependent variable, and independent variable. The specific objective of this lab is to determine whether certain human body parts experience allometric or isometric growth. Allometric growth defines when certain parts of an organism grow at unequal rates in comparison to its whole, while isometric growth is when all parts of an organism grow at the same rate in comparison to the entire organism. The specific purpose of the lab is to determine whether or not specific human body parts experience allometric or isometric growth by comparing the ratios of height to two specific body parts, in the students’ case the right hand length and head circumference, in students and newborns. The students formulated the tentative answer that if a team of four compared their height to right hand length ratio, as well as, their height to head circumference ratio, to those of a newborn’s, then the students will discover that the right hand and head experience allometric growth in humans.

Since body condition is a consequence of the level of nutrition, comparing the animals BCS with target values allows for decisions such as altering the plane of nutrition to be made.

Male C57BL/6J mice (No. 11401300006402), weighing 20 ± 2 g, will be used for this experiment. 7-month old mice will be maintained in a room at 23 ± 2°C and a relative humidity of 50 ± 10%, with a natural light-dark cycle. Food (6 mg thiamine-HCl as the form of TPP) and water will be provided ad libitum. After acclimatization for 1 week, the mice will be divided into two groups (n = 10 in each group) based on dietary difference: the dietary TPP depletion group (no TPP added) and the dietary TPP supplementation group (6 mg thiamine-HCl/kg diet as the form of

We are required to obtain certain amount of nutrients from food. Food can simply divide into 2 groups, macro-nutrients and micro-nutrients composition. Both of the groups mentioned do affect the metabolism rate. According to Hulbert and Else, dietary fatty acid will affect the metabolism rate and some of the polyunsaturated fatty acid will increase the metabolism rate (1999). Meanwhile, different kinds of animals have their own unique requirement for macro-nutrients, such as proteins, carbohydrates and fat. Moreover, most of the food intake is driven by achieving the required protein intake. Animals on a low protein diet will over eat carbohydrates and fat until the protein requirement has been satisfied. If this case