Secondary Modifications Of Red Cell Membrane

Sickle Cell anemia is a group of inherited red blood cell disorders, or a collection of recessive genetic disorders characterized by a hemoglobin variant called Hb S. Normal red blood cells are round like doughnuts, and they move through small blood tubes in the body to deliver oxygen. Sickle red blood cells become hard, sticky and shaped like sickles used to cut wheat. When these hard and pointed red cells go through the small blood tube, they clog the flow and break apart. This can cause pain, damage and a low blood count, or anemia. There is a substance in the red cell called hemoglobin that carries oxygen inside the cell. One little change in this substance causes the hemoglobin to form long hard rods in the red cell when it gives

The impeded blood flow also causes swelling of the patient 's hands and feet. In addition, the abnormal shaped red blood cells cause damage to the spleen, an organ that helps fight against infection (4). Subsequently, patients are more susceptible to contracting infections that require antibiotics and/or vaccinations. As mentioned earlier, red blood cells are produced in the long bones of the human body. The long bones are the bones that contribute most to height; because the red blood cells are impaired, long bone growth is impaired and thus affects overall growth (4). Furthermore, some patients report vision problems as a symptom. Small blood vessels supply the eyes with blood and oxygen, because blood flow is impeded to small blood vessels, vision is impaired (4). If a person experiences these symptoms, they may be diagnosed with SCD. A blood test can be performed to confirm whether or not a person has SCD. A blood test can detect hemoglobin S, the form of hemoglobin that is attributed to sickle cell anemia (4). This type is test is usually performed as a part of newborn screening and similar tests may be performed in older children and adults.

The disease is caused by a mutated version of the gene that help makes hemoglobin. Hemoglobin is the protein that carries oxygen in red blood cells. Sickle cell is a disorder in the blood caused by abnormal hemoglobin proteins. The abnormal hemoglobin protein caused sickle cell shaped red blood cells. The sickle cell red blood cells are crescent shapes and can clog up very easily in blood canals. In the diagram to the right it shows how a mutation causes a change in the amino acid.

Sickle cell anemia (SCA) is an autosomal recessive genetic disorder. This missense mutation is characterized by mutant beta globin subunits that tend to stick together (Cummings, 2014). As a result, abnormally shaped red blood cells are produced by this disorder. The erythrocytes are sickle or crescent shaped. Sickling occurs under hypoxic conditions, in which there is insufficient supply of oxygen delivered throughout the body (Sun & Xia, 2013). In order to inherit this monogenic disease, one copy of the sickle globin gene from each parent must be passed on to the offspring (Ashley-Koch,

The proteins of Bovine red blood cell (RBC) membranes were analyzed using polyacrylamide gel electrophoresis. After analyzing Bovine RBC they were then compared to human RBC counterpart. Following finding the log of each molecular weight of each band, band one showed the highest molecular weight. All five bands viewed were from humans. There are typically 7 bands visible however, in our case only five were visible due to implications within the gel sample.

Sickle cell anaemia is a disorder affecting the red blood cells and up to 100,000 people are estimated to have the disease in the USA (Centre for Disease Control). Due to a genetic mutation in the gene responsible for the production of haemoglobin (MayoClinic, 2014) the red blood cells, which are usually a biconcave disk in shape, become sickle (bent) shaped (Figure 1) and the haemoglobin that the red blood cells contain is defective. Haemoglobin is a protein that carries oxygen (which has bind to the haemoglobin) around the body from the lungs after gas exchange. Having defective haemoglobin, as is the case with sickle cell anaemia, means

All of the cells within the tissues of the body need a regular supply of oxygen to function. The hemoglobin found in red blood cells (RBC’s) are essential for the proper transport of oxygen molecules. They bind oxygen in the lungs and carry to every tissue within the body. Due to the structure of hemoglobin, normal RBC’s are donut shaped with a concaved center. This flexible shape is what allows the BRC’s to move freely into very small spaces such as capillaries where they will deliver their oxygen. Sickle cell anemia or other diseases that fall within the broader group of disorders characterized by SCD describe conditions where the protein (hemoglobin) has misfolded and altered the shape of the cell. Sickle hemoglobin allows for the formation of stiff rods within the red blood cell giving it a sickle, or crescent shape. This new shape is not flexible and can block or slow the flow of blood. When this occurs, oxygen cannot reach its intended tissues. This results in severe pain called crises, and these attacks occur

Normal RBCs are disc shaped in which they are able to easily travel throughout the body, especially through the tiniest blood vessels. However, with sickled shaped RBCs, they clump together while flowing through these particularly small vessels, which causes the loss of oxygen throughout the tissues and organs. This monogenetic disease is caused by a point mutation in the β- globin gene that creates abnormal Hemoglobin S (HbS). There are five different haplotypes for SCD that are classified by their geographical region

The disease I choose to talk about is Sickle Cell anemia, which is a blood disease that is prevalent among the African American race. This disease is interesting to me because it is a hereditary disease, so it can only be contracted if your parents have the disease. Sickle Cell anemia is caused by abnormal hemoglobin, the red blood cells in your body contain two vital hemoglobin molecules, a protein that carries the oxygen through your blood to travel throughout the body. People with sickle cell anemia, they have two copies of abnormal hemoglobin referred to as hemoglobin S. The molecule is what causes the problems within the red blood cells, rendering them unable to properly complete the process of carrying oxygen where it needs to go.

Sickle cell anemia (SCA) is one of the most common severe monogenetic disorders in the world. (1).SCA is an inherited blood disorder caused by a single point mutation in one of the genes encoding hemoglobin (2).Hemoglobin (Hb) it is an oxygen transporting molecule, carrying oxygen from the lungs to the tissues and takinge carbon dioxide away from the cell. Hb, made of four globin protein chains two alpha and two beata chains (pic).http://themedicalbiochemistrypage.org/images/hemoglobin.jpg

Sickle-cell disease majorly affects the hemoglobin that is present in our blood. The job of hemoglobin is to help transport oxygen and carbon dioxide to and from the cells throughout our body. Hemoglobin is present specifically in our red blood cells. Each red blood cell contains two hundred and eighty million hemoglobin molecules. Red blood cells normal shape is a biconcave shape because of the lack of many organelles and a nucleus. The shape is so important to a red blood cells functioning that if it is not shaped normally it has major consequences. The shape helps them to fit through capillaries easier and also allows for an increased surface area which results in easier gas exchange. Sickle-cell disease is a genetic disease that causes issues in the oxygen/carbon dioxide carrying hemoglobin molecules that are present in our red blood cells.

What is Hereditary Spherocytosis? Hereditary Spherocytosis is a rare type of blood disorder that typically affects the red blood cells. The person affected by this disorder will generally be considered anemic due to the red blood cells breaking down in such a short time frame of only 5-10 days verses the normal healthy person’s red blood cells breaking down over a longer time period of 50-60 days. A person with this particular disorder will more than likely have an enlarged spleen (splenomegaly) which would mean that this person will need to have a splenectomy done in order to keep the spleen from rupturing. The signs and symptoms of Spherocytosis can vary depending on the severity which ranges

Hereditary Spherocytosis is a blood disorder caused by a genetic defect in the genes passed down from someone in your family. These defects can cause the Spherocytosis to be passed down for many generations depending on how severe the defect was that was inherited. Even so, only 1 person out of 2,000 people will have this blood disorder as it is a very rare disorder. Although it is very rare, it is a very interesting type of blood disorder.

The purpose of this experiment was to determine the effects of tonicity on a cell membrane using red blood cells, potato strips and three unknown solutions (A, B, C). First three slides were prepared containing RBC’s and unknown solutions A, B and C. A control slide was prepared only using RBC’s. After observing each slide under the microscope it was determined that unknown solution A was hypertonic because the RBC appeared to have shrunk. The RBC in unknown solution B appeared to be swollen, therefor, the tonicity of unknown solution B was hypotonic. Unknown solution C showed no change to the RBC shape, it was suggested that unknown solution C was isotonic. To confirm the tonicity

In humans, mature red blood cells are flexible biconcave disks that lack a cell nucleus and most organelles. 2.4 million new erythrocytes are produced per second.[1] The cells