Selection Of Yeasts From Local Dairy Products Essay

The results for Benedict’s test for reducing sugars before hydrolysis, the control dH2O had no color change, as well as sucrose and raffinose. Gelatin became a dark blue and egg albumin a light greyish blue. Glucose yielded a dark orange, milk albumin turned orange, and starch had a yellow precipitate. In the Benedict’s test for reducing sugars by hot acid hydrolysis, the control dH2O was blue or no reaction. Glucose turned a brownish-orange; sucrose, a reddish-brown, raffinose, light pink; starch, a dark yellowish-orange; gelatin, violet; milk powder, a light yellow; and egg albumin, a greyish-violet. In the Lugol’s test for polysaccharides, the control dH2O turned yellow or no reaction, as well as glucose, sucrose, raffinose, milk powder and albumin. Starch was clear with blue-violet on the bottom (precipitate) and gelatin turned a slightly opaque white. In the test for polysaccharides remaining after hydrolysis with Lugol’s solution the control dH2O was yellow or no reaction. Glucose, sucrose, raffinose, starch and gelatin also had no reaction. Milk powder had a white precipitate and particle suspension. Egg albumin had an even greater white precipitate and particle suspension. In Biuret’s test for proteins there was no reaction for dH2O, glucose, sucrose, raffinose, and starch. Gelatin, milk powder and egg albumin all turned slightly violet with bubbles.

1) Amylase is utilized to break down starch molecules into more simple sugars for use by the body. It performs this function by hydrolyzing glycosidic linkages in the polysaccharide chain.

Specifically, alpha-amylase is produced by the salivary glands of Homo sapiens (Humans) as well as many other mammalian species and is encoded by the gene AMY1A (Tracey 2017, p.22). The enzyme alpha-amylase is able to uptake polysaccharides including starch and glycogen as a substrate then hydrolyze the alpha-1,4-glycosidic linkages that connect the monosaccharides together (Tracey 2017, p.37). This is the reason as to why salivary amylase is also referred to as alpha-amylase (Tracey 2017, p.22).

specific enzyme (Knowles, 1991). One part of the enzyme, salivary amylase, is that alpha amylase is in the saliva of most animals because this enzyme breaks down starch (Jacobsen, Melvaer, Hensten- Pettersen, 1972). In the presence of starch, this enzyme is present in saliva, but is not present when there is no starch present (Jacobsen, Melvaer, Hensten- Pettersen, 1972). The conditions for salivary amylase to have a reaction with starch would change in temperature and enzyme concentration, as well as, monitoring the pH levels (Jacobsen, Melvaer, Hensten- Pettersen, 1972). Salivary amylase is an enzyme is human saliva that helps in digestion of specific substrates, such as starch (Hudman, Friend, Hartman, Ashton, Catron, 1957). It breaks down starch molecules by splitting maltose from the non-reducing end of a gluten molecule (Jacobsen, Melvaer, Hensten-Pettersen, 1972).

In this lab experiment the action of the enzyme Amylase was observed on starch (the substrate). Amylase changed the starch into a simpler form, the sugar maltose, which is soluble in water. Maltose then breaks down the glucose chains of starch in the pancreas and intestines. Amylase is present in human saliva, and begins to act on the starch in the food while still in the mouth. Exposure to heat or extreme PH (acid or base) will denature proteins. Enzymes, including amylase, are proteins; if denatured enzymes can no longer act as a catalyst for the reaction. In the presence of potassium iodide, starch turns a dark purple color; however maltose does not react with I2KI. The rate of fading of starch allows a quantitative measurement of reaction rate.

A polysaccharide is when a numerous amounts of monosaccharides are joined together forming a molecule. Both glycogen and starch are polysaccharides. Glycogen is a energy storage carbohydrate found in animals. Glycogen in comparison to starch is much more highly branched. Starch is a polysaccharide that functions as a energy carbohydrate store in plants. This can be found mostly in potatoes and cereals. It is formed by the bonding together of many of glucose subunits into long chains. The actions of hydrolysis is the chemical breakdown of a compound due to the reaction of water. In order for hydrolysis to occur water must be added to the compound

In the past this experiment has been done. It can be found when you look up the title Yeasty Beasties. On the website, https://www.scientificamerican.com/article/single-celled-science-yeasty-beasties/, I found that this website was doing the project the same way I will be doing mine. After reading this article I have learned that if you mix sugar and yeast the yeast will eat the

In testing the differences that pH and temperature place on the enzyme, it will be possible to deduct the changes in enzymatic activity because of their surroundings. By using starch, a molecule that is hydrolyzed by amylase, it is possible to see the disappearance of substrate and creation of product with the addition of Lugol’s iodine (Fox 64). Adding the starch to the enzyme solution in the experiment allows for comparison between differing pH levels as well as the different temperatures that one might find amylase. It will be possible to determine what temperature and pH level are most effective for the enzyme to function at by running these comparisons.

Enzymes are biological catalysts, mainly proteins for this experiment, generated by an organism to speed up chemical reactions. They have active sites on which the substrate is attached, and then broken up or joined. For this experiment we are going to work with the enzyme a-amylase. Amylase is an enzyme that breaks starch down into sugar. Amylase is present in human saliva, where it begins the chemical process of digestion. Foods that contain starch like rice and potatoes. Amylase turns some of their starch into sugar in the mouth. The pancreas makes

Is dry yeast actually alive? Well, yes, if the right ingredients are added, then BAM, the mixture will turn into a bubbly, oozing mess of life! Yeasts are microorganisms, which are closely related to mushrooms and other fungi. For as long as it’s been known, yeast has been used to make pastries and many alcoholic drinks. The way that yeasts create their food (being the producers that they are), are by breaking sugars and starches down. In similarity to humans, yeasts must consume nutrients. Although in contrast to humans, yeasts must produce their food from their surrounding area to grow and reproduce, aka, to make more yeast. What is it that they eat; yeasts get their nourishment from sugars and starches (as briefly stated before), this

In this lab our group observed the role of pancreatic amylase in the digestion of starch and the optimum temperature and pH that affects this enzyme. Enzymes are located inside of cells that increase the rate of a chemical reaction (Cooper, 2000). Most enzymes function in a narrow range of pH between 5 through 9 (Won-Park, Zipp, 2000). The temperature for which enzymes can function is limited as well ranging from 0 degrees Celsius (melting point) to 100 degrees Celsius (boiling point)(Won-Park, Zipp, 2000). When the temperature varies in range it can affect the enzyme either by affecting the constant of the reaction rate or by thermal denturization of the particular enzyme (Won-Park, Zipp, 2000). In this lab in particular the enzyme, which was of concern, was pancreatic amylase. This type of amylase comes from and is secreted from the pancreas to digest starch to break it down into a more simple form called maltose. Maltose is a disaccharide composed of two monosaccharides of glucose. The presence of glucose in our experiment can be identified by Benedicts solution, which shows that the reducing of sugars has taken place. If positive the solution will turn into a murky reddish color, where if it is negative it will stay clear in our reaction. We can also test if no reduction of sugars takes place by an iodine test. If starch is present the test will show a dark black color (Ophardt, 2003).

Fermentation a metabolic process with occurs in the absence of oxygen molecules also known as an anabolic reaction. It is a process of glycolysis in which sugar molecules are used to create ATP. Fermentation has many forms the two most known examples are lactic acid and alcoholic fermentation (Cressy). Lactic acid fermentation is used in many ranges from food production such as bacteria to its use by fatigued muscles in complex organisms (Cressy). When experimenting with organisms such as yeast which was done in this experiment you follow the metabolic pathway of Alcoholic fermentation (Sadava). Where the sugar molecules are broken down and become ethanol (Sadava). But the end product of fermentation is the production of

In bread making for example addition of amylases can yield more sugars from the starch in flour and barley grains, another commercial which is statue of import ants is the conversion of starch to sweet glucose syrups which are used generally as sweeteners in food industry as well as in the bread-making and brewing industry. Altering the balance between amyloglucosidase and the fungi alpha amylase can produce different proportions of glucose and maltose. A higher proportion of glucose is useful for fermentation whereas higher maltose is more useful in preparation of jam and confectionary. The Amylase Enzyme breaks down starch; which is a carbohydrate.

The objective of this lab was to assess the effect that temperature and pH had on pancreatic amylase. Amylase is an enzyme involved in digestion and is secreted by both the pancreas and salivary glands (Pandol, 2010). Both salivary and pancreatic amylase display the same enzymatic activities through their role in the digestion of starch (Pandol, 2010). The goal of both salivary and pancreatic amylase is to hydrolyze starch thus breaking down the polysaccharide into a disaccharide such as maltose (Pandol, 2010). As a result, amylase breaks down these sugars making them more easily absorbed in the body during digestion (Pancreatic Cancer Action Network, 2015). Through this lab we learned about the role of pancreatic amylase and how this enzyme operates under certain conditions.

The dough must contain some alpha amylase to be able to digest the portion of starch that is amylopectin. If the alpha amylase is too much in the dough the starch will completely liquify. Alpha amylase attacks starch virtually wherever along its chains, producing smaller chains of different lengths. These chains can hold smaller units called dextrin’s that contain numerous units of glucose. Beta amylase can then digest these dextrin’s into maltose.