Seven Agar Plates

4. Using a cotton swab, evenly spread the bacteria on the agar of each plate, making sure to open the lids as little as possible.

In the beginning of lab, we were advised to obtain a nutrient agar petri plate, which is used for the cultivation of microbes supporting growth of non-fastidious organisms. Since it contains many nutrients, a wide variety of bacteria and fungi can grow. Taking the plate,

2) Record the shape of the bacteria, the arrangement of the bacteria, and the gram staining characteristics.

Read in your lab manual about the following agar mediums: Blood Agar (pg 168), EMB Agar (pg 170), Mannitol Salt Agar (MSA)(pg 172) ), MacConkey Agar (pg 174), and PEA Agar (pg 176) to answer the following:

The unknown project was an experiment where the student was given a petri dish of unknown bacteria. Tests were performed on it to identify the specific species. The purpose of the experiment was to learn about the identifying tests, and procedures in the identification of specific microbes. The reason the master plate was used to create a working plate is so that if the working plate becomes contaminated, one can resort back to the master plate for the pure strain of the bacteria and create a new working plate. The purpose of the first procedure, the gram stain, was to be able to dye and then distinguish gram negative and gram-positive cells on a smear. The second procedure, the citrate test is used to see if the bacteria can use citrate as

Use the cotton swab to streak the five labeled nutrient agar plates with the E. Coli K12 bacteria from the tube.

To do the lab you will need granulated sugar. 50 mL of cold water, room temperature water and hot water. Cups for the water to go into. A glass beaker to pour the hot water. A balance, thermometer, wax paper, stopwatch,spoon, and a straw to stir the sugar.

All it takes is one single cell to start a pure bacterial colony. This lab allows researchers to observe isolated highly concentrated samples of bacteria, to view their traits and to isolate pure colony samples for use in future experiments. Unfortunately successful isolation was not achieved in this lab. Possible sources of error could be that the streaking was too close together resulting in an unsuccessful isolation, the loop was not flamed adequately which permitted carryover of bacteria from one sector to the next or forgetting to let the loop cool before touching the agar. To expand this lab, researchers could collect a single colony from an agar plate and grow it inside a nutrient tube to observe the oxygen nutrient requirements for that specific sample of

Methodology: • Food items to be tested (ham, cheese, chip). You will need four of each. • Sterile swabs • Sterile gloves • Timer • Agar plates (you will need 12 of them.) • Notebook to record results Experimental Procedure: 1. Put on sterile gloves ( do not touch any non-sterile surfaces when wearing gloves) 2.

In this experiment, each student was randomly assigned with a different species of gram- negative bacteria. The organism that I was assigned was Unknown #16. The identity of the gram-negative bacteria was determined to be Escherichia coli. The purpose of this report is to describe the various tests that helped develop a better understanding of the unknown microorganism in terms of the physiology, morphology, motility, and antimicrobic sensitivity it is characterized with. Indole production, hydrogen sulfide, and the colony morphology on the Eosin-methylene blue (EMB) plate, were the critical results that led to the conclusion that the organism was E. coli. In the indole production test, E. coli was one of two organisms,

In this experiment some mistakes and errors that could have impacted on the results. Firstly, it was really difficult to place the amoxicillin paper to the agar plant. Some amoxicillin was smeared some place of the agar plate by mistake, which would influence the result. Furthermore, the discs were put on the table, where are lot of different micro-organisms. Moreover, the agar plates were open when they were put on the disk for a long time after the E. coli have spread around them. Some micro-organisms that are floating in the air could have been place on the agar plates. One agar plate was damaged because smear the E. coli too hard. These kind of human mistakes were all caused by incaution of the operators. The experiment should be done more

The experiment was completed by first putting on safety goggles and an apron, then get a sharpie and draw a cross on the top of the petri dish that has agar in it, then label each quadrant with one control group. After that has been completed the partner will need to clamshell open the petri dish while the cotton swab dipped in Micrococcus luteus is being spread side to side from the top of the agar to the bottom. When that is completed the petri dish is going to need to be turned ninety degrees and the same procedure will need to be performed. Once the bacteria is spread across the agar it will need to be taken to the counter with six household products on it and put one household product in the first quadrant, another in the second quadrant,

The lab consisted of several materials such as test tubes, test tube clamps, graduated cylinder, beakers, hot plates, test tube rack, pipet rubber bulbs, porcelain test plate, medicine dropper, pipettes, thermometer, and a funnel. The solutions and temperature based materials required 0.5% amylase solution, distilled water, 0.5% starch, iodine-potassium-iodide, ice and benedict solution. While

Items needed for this experiment are: 6 petri dishes that have been prepared with nutrient agar and made into plates, then labeled 10-1 through 10-6, 6 test tubes labeled 10-1 through 10-6 then place in test tube rack, sterilize graduated pipet with alcohol, be sure to remove all of the alcohol from pipet, distilled water, 1 disposable cups, 1 yeast packet, permanent marker, paper towels, bleach, safety gloves, goggles, face mask and apron.

To start off the experiment, materials were gathered and placed on the table, those materials were 6 different essential oils. Peppermint, lavender, sweet orange, tea tree, lemon and eucalyptus were all used in the experiment. After collecting the materials, the containers were labeled with the name of each essential oil and the agar was then heated using the microwave until it turned into liquid. The agar was then placed in the six different petri dishes and add three drops of each of the essential oil to its labeled petri dish. After the agar had solidified and cooled down it was placed in a room temperature and pictures were taken. Growth would be observed every two days by taking pictures and if growth is present, then the names of bacteria