Sex And Phenotype Of The Mutant Fly

The total number of Pago Fuagans that are carriers of the (g) allele and phenotypically normal =

parent carried the b allele. The F1 offspring of such a cross would be Bb, and

Calculate the ratios of the genotypes and phenotypes of the offspring in the F1 generation.

It was decided that there would be 80 vestigial flies and 20 wild type flies to total to an initial population of 100 drosophila. Next, the flies were anesthetized flies using Fly Nap. The flies were counted out to reach desired ratio, sexing the flies making sure there are equal amounts of males and females to be sure there is ample individuals to allow successful mating. The fly’s food was prepared by taking a frozen rotten banana, cutting it in half, mashing up the banana meat, and mixing yeast into it. The

In our experiment, we created three crosses; DxG,BxD, and BxG. In crosses BxG and BxD we found a rare mutant fly. This unusual mutant had a misshapen abdomen, deformed wings, and was pale in color. While the mutant was rare, due to the fact that it popped up in both those populations, we hypothesized that this genetic mutant had already previously been discovered and named.

Gathering Data on the Different Traits of the Garden Pea (Pisum Sativum), Organizing the Dominant/Recessive Phenotypes of 60 F2 Offspring and Determining Whether the Null Hypothesis is Rejected or Accepted Using the Chi-Square Test.

You are also provided with a heterozygous female, and a homozygous recessive male for a genetic cross. In this particular female, all the dominant alleles are on one chromosome, and the recessive counterparts are on the other homologous chromosome. Due to a chromosomal condition, in the female no recombination occurs between the M and N loci. Normal recombination occurs between the L and M loci. Diagram this cross, and show the genotypes and frequencies of all offspring expected from this cross.

METHODS: In this experiment, the instructor provided us with 30 ebony individuals and 20 wild type individuals. In order to get an exact amount of each type, we anesthetized the flies and counted them off by gently using a fine point paint brush. Then all 50 Drosophila were put into a population cage which had a lid that had six holes for the centrifuge tubes. Two food tubes and four clean, empty tubes were added on the first day. Each food tube consisted of half a cup full of food mixed with 6-7 milliliters of water. This was the fly medium. The food should turn blue once the water is added. Each tube was labeled with a number and with the date. Every two to three days we added one more food tube until all 6 tubes contained the fly medium. After all 6 tubes were filled, the following days after we exchanged the first food tube with a new food tube. At the end of the experiment, we fed the flies with a total of 8 food tubes. Then the flies were anesthetized, again. At the end of this four week lab, the number of living ebony and wild

we said goodbye and placed them in the fly morgue. We allowed the F2 larval

In cross B, a red-eyed female fly was placed into a vial with a white-eyed male fly and a red- eyed male fly for a breeding period of two weeks. At least eight red-eyed females were produced from this cross. These eight females were used to conduct a polymerase chain reaction. Comparing to the control groups, the results show that seven out of eight flies has the Xw+ Xw genotype because all of them had both bands appear on the gel when both alleles were present. This indicates that all of them are heterozygous. They are all red-eye females. Fly 7, however either of the band appear. This means that an error occurred, and one possible error could be not enough DNA were extracted therefore the gel could

5. Again, set the number of offspring to the maximum of 6. Then, click the Cross button repeatedly until these parents have produced about 100 F1 offspring.

Our group set up 7 tubes of female wild type and male sepia eyes/ebony bodies. The group also set up three tubes of female ebony and male vesitgal. It is important that for every one male you place in the tubes you place three females in as well. The number of tubes that were created were based off the number of flies that were given to the group. Table 1 shows the type of fly and how many of each.

Intro: In this laboratory experiment we cross bred fruit flies (Drosophila melanogaster) in order to identify which traits were passed onto their offspring. Since the 20th century the common fruit fly has been a useful and one of the best organisms for the study of genetics. Thomas Hunt Morgan was responsible for the work that demonstrated the huge advantages of fruit flies and led to them becoming a common model for scientific research and testing. Its relatively short generation time (~2 weeks) produces a high rate of reproduction and since they are small and easy to grow, they make for a cheap and simple experiment to do in the laboratory. They also only have four pairs of chromosomes which allow being able to detect which traits pass on to their offspring quite easy.

For our first generation (F1) of flies we chose to cross apterous (+) females and white-eye (w) males. We predicted that the mutation would be sex linked recessive. So if the female was the sex with the mutation then all females would be wild type heterozygous. Heterozygous is a term used when the two genes for a trait are opposite. The males would all be white eye since they only have one X chromosome. If the males were the sex that had the mutation then all the flies would be wild type but the females would be heterozygous.

This experiment looks at the relationship between genes, generations of a population and if genes are carried from one generation to another. By studying Drosophila melanogaster, starting with a parent group we crossed a variety of flies and observe the characteristics of the F1 generation. We then concluded that sex-linked genes and autosomal genes could indeed be traced through from the parent generation to the F1 generation.