Similarities Between Prokaryotic And Eukaryotic Cells

• *Compare and contrast the structure and function of DNA and of RNA molecules. Reference Table 4.1.

Ribosomes in any type of organism are all the same, but we distinguish between two

1) DNA programs protein production in the cytoplasm by transferring its coded information to a molecule called RNA (mRNA). The RNA then carries the order to build this type of protein from the nucleus to the cytoplasm.

Since DNA has the instructions for making protein we usually wonder how is it able to make ribosomes if DNA is stored within the nucleus. This is when a handy tool comes in called transcription and copies the DNA into mRNA so it can be reached outside of the cell.

This paper explores the history and some interesting facts about DNA. The last couple centuries have seen an exponential growth in our knowledge of DNA. The history of the DNA can be traced back to multiple devoted scientist. This article attempts to summarize, and review the basic history of DNA while providing some fascinating information about it.

There are two main types of cells in the world. The simplest cells such as bacteria are known as Prokaryotic cells, and human cells are known as Eukaryotic cells. The main difference between each of these cells is that a eukaryotic cell has a nucleus and a membrane bound section in which the cell holds the main DNA which are building blocks of life.

3. What needs to land on the pink part in order for the gene to be expressed? RNA Polymerase

Furthermore, DNA is found in large quantities within the eukaryotic cell. Human cells alone have around 1000 times more DNA than typical bacteria [Alberts, c1989, p.23]. DNA, both on its own and with other molecules, plays a huge role in the making of an organism, from the importance of its chiral helical structure and its main functions, to the vast vicinity of error and inaccuracy that a small change to the genomic sequence can cause. It is the foundation upon which an organism is built and the main contributor to an organisms genotype and phenotype. But what are the constituents of DNA and can it

While we had performed sub cellular subscription function before the function of RNA isolation in 15 cell lines to deeply interrogate the human transcription. Now for the K562 lines we had perform the additional nuclear sub fractionation into the chromatin, nucleolus and the nucleoli. The RNA from each of these sub compartment were prepare in the form of replica and were separated from each other on the basis of the length into >200 nucleotides long and <200 nucleotides short. The part consisting of the long RNA were again been further fractionated into polyadenylated and in the form of non polyadenylated transcripts. There was a use of various number of complementary technologies were been use to characterize these fractions of RNA to their sequential order was made. Sequence rewards were been mapped and were been proceed through the use of a variety of tools also called as the software tools. We have been used to map the data to assemble those and quantify De novo elements. For the process of reproducibility the elements and the quantification were been further assessed between replicates and non parametric version of the irreproducible detection rate for the statistical test. The most part of the analysis with at least 90% irreproducible. Then the data which are been

Escherichia coli K12 is a well-studied gram-negative bacteria. First isolated from the human gut, is one of the most used in molecular studies, being the knowledge obtained from studies of E. coli possible to apply to other organisms (Burton and Kaguni, 1997). Some enzymes have a crucial role in the transcription control, the topoisomerases enzymes play an important role in the level of DNA supercoiling, an important property of DNA and chromatin (Gilbert and Allan, 2014). The supercoiling level is modified by a reaction that consists in the transient breakage of the DNA phosphodiester bonds and the movement of strands across the transient breaks (Tse-Dinh and Wang, 1986). In prokaryotes, these enzymes can be classified into two major groups, according to the mechanism of action. While the topoisomerase II (DNA gyrase) generates negative supercoils into relaxed DNA and relax positively supercoiled DNA, the topoisomerase I relax negatively supercoiled DNA. It is important to equilibrate the superhelical state of the cellular DNA (Hirose and Matsumoto, 2000).

3. Tortora GJ. Functional Anatomy of Prokaryotic and Eukaryotic Cells. In: Microbiology an Introduction. 9th ed. San Francisco, CA: Pearson Education, Inc; 2007: 77-113.

Prokaryotic Cells All living things are made of cells, and cells are the smallest units that can be alive. Life on Earth is classified into five kingdoms, and they each have their own characteristic kind of cell. However the biggest division is between the cells of the prokaryote kingdom (monera, the bacteria) and those of the other four kingdoms (animals, plants, fungi and protoctista), which are all eukaryotic cells. Prokaryotic cells are smaller and simpler than eukaryotic cells, and do not have a nucleus. Prokaryotic means 'pre-nucleus' and eukaryotic means 'true nucleus'.

Transcription is where DNA is transcribed into RNA which then can be pass to the ribosome’s to act as a template for protein synthesis. Before transcription can begin DNA must unwind and the two halves of the molecule much come apart so exposing the base sequence. This process begins when a region of a two DNA strands is unzipped by enzyme called RNA polymerase attaches to the DNA molecule at the imitation site.

The process of DNA replication plays a crucial role in providing genetic continuity from one generation to the next. Knowledge of the structure of DNA began with the discovery of nucleic acids in 1869. In 1952, an accurate model of the DNA molecule was presented, thanks to the work of Rosalind Franklin, James Watson, and Francis Crick. To reproduce, a cell must copy and transmit its genetic information (DNA) to all of its progeny. To do so, DNA replicates following the process of semi-conservative replication. Two strands of DNA are obtained from one, having produced two daughter molecules that are identical to one another and to the parent molecule. This essay reviews the three stages

Another cellular feature shared by both Archaea and Bacteria is size and arrangement of ribosomes. Their ribosomes are much smaller in comparison to eukaryotes. The function of their ribosomes is similar to the ones in eukaryotes; for translating mRNA codons to sequence of amino acids for the synthesis of proteins. Both have 70S Ribosomes composed of 30S and 50S sub units that are joined to make a 70S unit. They contain “three ribonucleic acid molecules” consisting of “16S, 23S AND 5S”. On the other hand, the “primary structure of Archaea r-RNA and r-Proteins” is much similar to the ones in eukaryotes and less similar to that of bacteria. Additionally, the Archaea ribosome is much firm compared to mesophilic bacteria’s ribosomes, this is particularly beneficial in terms of their adaptation to extreme environmental conditions. (Archaeal Ribosomes, Paola Londei, university of Rome, “Sapienza”