Simulated Gastric Report

Three ways to assess water are positive/negative, membrane filtration and IDEXX Colilert and Enterolert assays. For membrane filtration, petri dishes and an incubator are used. For IDEXX coliert powdered medium is used for total coliform and E. Coli, while Enterolert media is used for enterococci. Also an incubator, colilert trays are used. IDEXX is more expensive than membrane filtration.

Using the information found in the Gram Negative Enteric Baccilli and Gram Positive Cocci Reference books,

The next step of the project included preparing a Gram stain to discover the cell shape, arrangement, and if the bacteria is gram positive or

5 drops of chloroform were added to the mixture and incubated at room temperature for 15 minutes to kill, and lyse the bacteria cells which allows all the unadsorbed phages to be accounted for when determining the titer of unadsorbed phage with susceptible bacteria, E. Coli B. (Biology Dept.). 0.1 ml of E.coli B was added to the 10 fold dilution. Using soft agar technique, the unadsorbed phage were plated. After incubation, the titer of unadsorbed phage was determined by counting the number of plaques on the plate from the infection of single E. coli cells in the assay tube by a free phage particle before

In this experiment, we were given a sample of an unknown bacterium and asked to classify it based on its physical and metabolic properties. We hypothesized that the unknown organism was Alcaligenes faecalis, a motile Gram-negative bacillus that resides in water, soil, blood, urine, and feces (Herrera n.p.) It was first isolated in 1896 by Petruschky, who

The main idea of this experiment was to correctly identify the unknown bacteria, #3. Identification of unknown bacteria yields multiple benefits in many different areas in the research of microorganisms. In this experiment I performed many different test dealing with things such as the presence of enzymes, fermentation abilities and different chemical reactions. Observations made from the tests were then compared to a gram negative unknown chart in order to identify the bacteria. Based off of my results and the chart, I concluded the bacteria #3 was the bacteria Escherichia coli. E. coli is most commonly found in the intestines of warm blooded organisms. Most E. coli strands are non pathogenic however, there are strands

A gastric bypass weight loss program is for those who are recuperating from gastric bypass surgery to assist them to heal and alter bad eating habits. Gastric bypass surgery is among several weight-loss surgical procedures presently carried out. The operation itself has gone through several modifications through the years. The process being used today is known as the Roux-en-Y gastric bypass. It should not be mistaken with other weight-loss surgical procedures, like the biliopancreatic diversion with duodenal switch, that is a more aggressive surgery. Your physician or perhaps a registered dietitian will talk to you concerning the diet you will need to follow after surgery, explaining what kinds

Abstract: The objective of this experiment is to find out what the optimal growth environment is for V. natriegens out of three different environments. The first is a Brain Heart Infusion broth (BHI) that contains 50 mM of NaCl. The second is BHI containing 250 mM of NaCl. The third is BHI containing 1000 mM NaCl. In order to obtain this, a growth curve graph must be constructed for each different environment, and the generation time of the bacteria in each environment must be calculated. The optimal environment for V. natriegens was found to be the BHI with 250 mM of NaCl.

Ever started gaining weight at age 10 after her mother died. She tried all sorts of diets and nothing seemed to work. After breaking a chair at a school assembly and a talk with her step-sister Brielle, Ever decided to undergo gastric bypass surgery. Some reasons for this surgery was to try out for the school play and to land a date to the school dance. A couple weeks after her surgery, she started losing a great amount of weight, but she couldn’t eat as much so she became famished all the time. People at school soon noticed her weight loss and she became very complacent with herself. Eventually, her outlook on life changed and so did her friendships. Shortly after the school year started, she got engrossed in the “popular” crowd. She soon

The thioglycollate broth showed that Clostridium sporogenes, Escherichia coli, Micrococcus luteus, and Enterococcus faecalis all showed growth that was throughout the tube but there was a slight inhibition of growth at the very top of the tube. Pseudomonas aeruginosa showed growth throughout all of the tube including the very top of the tube. With the TSA + glucose plate, all the bacteria but C. sporogenes was able to grow on the plate aerobically. For the plates that were grown in anaerobic conditions, only C. sporogenes, E. coli, and E. faecalis were able to grow on the plates.

In the 10-3 pasteurized sample, the plate exhibited 71,000 cells/mL. The results of the additional dilution samples contained too few colony forming units to count. However, in the 10-7 dilution, although the plate demonstrated 12 colonies, there should have been no colony forming units on this plate. The reasons for this could have been that this sample was contaminated from “double-dipping” the sample before dispensing it onto the plate or when using the pipette, it mistakenly was inserted in a higher concentration sample and then immediately to a lower concentration sample before it was dispensed onto the plate.

This lack of presence of coliforms may indicate the absence of fecal matter in our water sample, however; it does not indicate whether the water sample is safe for swimming in or drinking from. It is possible that the non-coliform bacteria, which was potentially incubated in lab, could pose a hazard to the public if consumed or if the microbes enter the body. Additionally, our results may be a false negative. It is possible that the time which passed between the collection and refrigeration time of the microbe was significant enough to cause the coliforms to die or be out performed by additional microbes living in the same sample. This could cause the numbers of the coliforms of interest to be so low our test was unable to detect their presence. To prevent this possibility in the future a cooler could be brought to the site of interest and the sample could be placed within the cooler immediately after collection. However, it is more likely that our sample simply did not contain coliforms and was not contaminated with fecal

Gastric juices of the stomach, by the action of hydrochloric acid or enzymes, kill most microbes.

First, dissection of the phrenoesophageal membrane and dislocation of the GEJ into the chest do not alter manometric LES pressures. Second, selective myotomy of the clasp fibers or selective excision of the gastric sling reduces LES pressure and compromises GEJ competence but does not totally abolish these, whereas myotomy or resection of both structures abolishes the manometric LES and GEJ competence.

Summary The article discusses about new scientist discovery of artificial mini-stomachs that is made in the lab. The artificial mini-stomach was made by stem cells consisting of tiny clusters of human gastric, which makes it the world’s first mini stomach. The human gastric was also used on the mini stomach and it is called organoids that is made form buds of cells that are a miniature version of the stomach. They were also made from pluripotent stem cells, which coaxed into developing into gastric cells according to the article. It was very difficult to study human stomach disease up until the invasion of the artificial Stomach. The discovery of mini stomach is identical to that normal stomach of the stomach in human body, which makes it easier