Slide Culture Observation Paper

2) Through microscopic examination of pollen or spores, it is possible to identify a specific plant that produced that pollen or pore.

The Olmec are believed to be the first great Mesoamerican civilization. The city known as San Lorenzo existed around 1200BC and declined in 400 B.C. It is considered to be the first major city in ancient Mexico.

In typical neutral soil, the numbers of bacteria dominate over the number of fungi. Because of this, general growth plates often see little to no fungal growth when placed in these conditions. In order to select for fungal growth, an acidic medium is normally used because fungi grow readily at higher acidity (S.A. Waksman, submitted for publication 1922). This information is useful in determining the type of medium required to select for fungal growth.

At optimum temps, and environmental conditions, with peak mycelium in culture, every 16-24 days. This in a very low tek indoor setup. Times are

Purpose: The purpose of this experiment is to gain knowledge of the functions and operations of the compound light microscope and an immersion oil lens by observing prepared slides of various bacteria and blood slides. We are also learning to indentify and observe the various shapes and characteristics of bacteria, as well as, yogurt cultures (fresh and prepared) and blood samples under a microscopic view. We will also be able to distinguish between blood cultures and bacteria specimens.

Describe the process of germination and plant growth you observed in the lab activity for a dicot plant.

The first was the culture tube set up. This involved adding dry media to a vial and mixing it with water until it solidified. After the vial is set, add one to two grains of yeast to the tube. The second procedure was putting the flies to sleep and observe the different phenotypes they had. This involves a chemical referred to as fly nap to put the flies to sleep.

5 The test microscope slide was composed of a drop of B. subtilis solution placed on the left as the Gram-positive control, a drop of a solution with unknown in the middle, and a drop of E. coli solution on the right as a Gram-negative control. The slide was allowed to dry and the samples were heat fixed with a Bunsen burner. The samples were then stained with first crystal violet for one minute. After the rinsing the stain, the mordant, Gram’s iodine, was added for one minute and rinsed. A 95% ethyl alcohol/acetone solution was then applied and rinsed to de-stain the samples. Finally, Saffranin was added as the counterstain for one minute and then rinsed. Bright field microscopy, using 1000X magnification and oil immersion, was conducted to visualize first the controls, and if those results were accurate, the unknown. The three samples were observed for stain color to determine Gram classification, as well as shape and arrangement to further classify the

For the endospore test, I used the Schaffer-Fulton spore staining method. I spread the bacteria on a slide and covered it with a piece of bibulous paper. I applied malachite green stain on top of the paper and steamed the slide for 6 minutes, reapplying drops of malachite green stain periodically. After steaming, I removed the paper, rinsed with distilled water, counterstained with Safranin for 1 minute, rinsed with distilled water again, and dried the slide with bibulous paper. I checked the endospore stain with Microscope

Spores can be stained using specific dyes, such as malachite green, that are absorbed by spores in the presence of heat (Noonan).

Before starting all the biochemical tests, an inoculation procedure must happen. An unknown test tube was handed out which contained two different cultures. The unknown culture was place on a nutrient agar plate using the three-streak inoculation technique for isolation (Lab Manual, Chess 2015). After a few days of growing, the presence of two unknown bacteria had a clear visible of separation. Following the isolation streak technique, the gram-staining experiment was performed. The two bacteria were determined: one was Gram-negative bacteria and the other was Gram-positive bacteria.

cerevisiae were obtained and labeled accordingly. These served as the starting point for the experiment. An untainted YPD plate is retrieved and sectioned into three areas and labeled: “A”, “B”, and “C”. A small sample of each strain of yeast is taken and lightly spread onto its own section of the YPD plate. The plate will then be left in the incubator at 30 ° C for 24 hours. In the time preceding time five more media plates are acquired and labeled according to a key that indicates the type of media it is. After the time of incubation the original YPD plate is then replicated on SD+His+Leu, SD+His+Ura, SD+His+Leu+Ura and YPD plates (YPD being the control). Each plate needs to be labeled in the same manner as the first media plate so observations can be correctly made. Each replica place should also be labeled according to which strains are going to be transferred to it. A cylinder shape block, with a compression ring is used along with a precut section of velvet in order to transfer an identical pattern of the yeast on the original plate. All of the plates are reentered into the incubator for 48 hours, then the new growth is observed and

Noted that all the steps above are done in the laminar flow to avoid contamination that might stunt the growth of A. flavus. 3.3.2 Spores Suspension Inoculum Preparation A. flavus from Petri dish was transferred

Spores were sown in the week of September 10th and no observations were made. First observation was made in the week of September 17th, Friday 3:08 pm, the control displayed some growth with flagella-like hair, however, no germination was observed. The treatment did not display any signs of growth, only air-like bubbles were observed. Second observation was made in the week of September 24th, Friday 3:10 pm, the control displayed germination, gametophytes were visible under the microscope, however, hermaphrodites were not significantly observed. The treatment still does not display signs of germination, only small green spores were observed under the microscope. Last observation was made in the week of October 1st, Friday 3:05 pm, the control displayed significant signs of growth, it appeared that fertilization took place and an embryo was in development. The treatment only displayed minimal germination, however, some spores appeared to be infested with fungus.

The purpose of this experiment is to obtain isolation of individual species of particles from the mixed culture. This is completed through the isolation technique of streak plate. The objective of this experiment is to replicate the technique of streak plate but on a much larger scale. Because it is on a larger scale the particles are able to be visually observed as they are isolated using the streaking technique as the experiment is conducted. The benefits of the streaking technique is when a cultures has multiple species they are able to be more easily identified once they have been isolated. This experiment is much like the experiments completed on an agar plate but on one a much larger scale and where techniques