TITLE
Lab #4 Soil –vs- Water Microbiology
INTRODUCTION
There are 4 learning goals for this lab:
1. Collect samples and compile data from at least 2 sources (soil; water) of your choice.
2. Identify at least 2 Prokaryotes (bacteria) and 2 Eukaryotes (fungus) in your samples.
3. Research the importance of bacteria and fungus to Earth in a non-medical context.
4. Compile a high-quality, 3+ resource (excluding the lab worksheet), MLA-cited in-text laboratory report using the scientific method by class due date.
HYPOTHESIS
The purpose of this lab is to:
1. Collect a sample of soil from (your school) on October 27th 2011 and collect a sample of pond water from (the name of your lake or pond) on October 27th and record the data
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Microbiology Introduction. 10th Ed.). It was no surprise to me that E-coli were found in the soil sample because of the geese and E-coli comes from fecal matter of animals. The soil we retrieved was from the area by the lake and therefore very possible to get a positive result for E-coli. All of the fungi and bacteria found from the environment share a symbiosis relationship, two organisms living together in a close association that is beneficial to one or both of them. (Tortora, Funke, and Case. Microbiology Introduction. 10th Ed.) The relationship that bacteria and fungi share with each other contributes to the biogeochemical cycle and without bacteria and fungi we could not continue our existence on our planet “earth.
METHODS AND MATERIALS MATERIALS 1 Becker 1 Nutrient agar plate 1 pair plastic gloves 1 Potato agar plate 1 Staining tray 100ml of distilled water 2 MacConkey Agar Plates 3 Blank slides 3 plastic cups 6 pipettes 95% Grams alcohol Bibulous paper Boat & Scale Bunsen Burner & Flint Crystal-violet Grams Iodine Incubator of 35⁰ Celsius (silver) Inoculating loop Microscope Pond water Safranin Soil Tap water Timer Tweezers METHODS Methods performed as per BIO 270 Soil sample on October 27th 2011 at 11:45 a.m. Water sample on October
2. Develop a hypothesis relating to the amount of dissolved oxygen measured in the water sample and the number of fish observed in the body of water.
To conduct your laboratory exercises, use the Laboratory Manual that is available in the classroom. Laboratory exercises on your CD may not be updated.
This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.
2. Develop a hypothesis relating to the amount of dissolved oxygen measured in the water sample and the number of fish observed in the body of water.
BEFORE CONDUCTING THE EXPERIMENT - You should already have submitted to your teacher an EXCELLENT Investigation Design that included:
This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.
Microbes are bacteria, archaea and eukaryotes. The earth was formed 4.6 million years ago. And a few million years later, by 3.5 billion years ago, earth was already inhabited by a diversity of organisms. The earliest organism is Prokaryotes and within the next billion years, two distinct groups of prokaryotes called bacteria and archaea diverged. Eukaryotes cell evolved from a prokaryotes community, a host cell containing even smaller prokaryotes .The microbial world accounted for all known life forms for nearly 50 to 90% of Earth's history. We are still researching microbial organisms today in marine environment, extreme environments. A microbial observatory is an NSF-funded project dedicated to the discovery and characterization of novel microorganisms and microbial communities of diverse
Abstract: Microorganism need to live in ideal conditions so they can grow. This experiment was performed to determine if there was a greater number of microorganisms in Winthrop lake than Winthrop wetlands. We determined this hypothesis because the lake was bigger. We also made the hypothesis that the pH level of the lake was going to be higher than the wetlands. We tested out the hypothesis by going out to Winthrop lake and wetlands and collecting samples of water. Back in the lab, we examined the samples under a microscope and recorded all the organisms we could find on Excel. Also, we tested the pH levels of the
The predator populace in the earth populace is developing, while the predator populace in the grass is not advancing as quick. Everybody and each living thing are always advancing; in any case, since the knifes in the earth populace could outlast the forks, dissimilar to in the grass specialty, they are developing quicker. The spoons still ruled over all, surrendering the opposition over to the blades and forks. Table 2a obviously demonstrates that the knifes cooperated better, or were forceful in their approach of attempting to survive longer, and making more eras. Similar phenotypes were not supported in both the grass and soil populaces on the grounds that distinctive results came to be. On the off chance that the knifes just murdered off the forks in the grass segment, then the phenotypes would have been the same. The way that the knifes adjusted their nourishment on their mouths and afterward ate it was a key make, that drove them beat the forks. Since in the grass segment the forks likewise could defeat the do not have that their mouths stalled out within the individual clear out.
Gather 20 clear cups and the fine grain sand, compost soil, and orange clay. The different soils will be measured in percentage of 100 mL. Use a black sharpie to label each cup to visualize the soil contents, six cups will be labeled 50-25-25. The percentages of each soil will change one cup will have 50% compost 25% sand and 25% clay. The other cup will have 50% clay 25% sand and 25% compost. the other cup will
Fungus attaches to the roots of plants to create a mutual interaction called mycorrhizae. This interaction is symbiotic and both organisms benefit. Funguses help expand a plants root surface area, so the roots can reach nutrients when the soil is depleted. Also, funguses use plant roots to obtain sugar and carbon dioxide. This interaction occurs in majority of all plants and is vital to plants survival and protection from pathogens.
Soil is everywhere but it is easy to ignore . There are so many types of soils . Soil contains very many fungi and microbes . Soil is also used for recycling by breaking down the leftover of the plant when it dies. It also breaks down the remaining animals after it
Collect the materials from designated spots. The rocks chosen for the experiment should have a number written on them. Be sure to list the number of each rock on the lab sheet. 2. Label a spot on the lab sheet for each partner in the group for both trials.
In order to assess the water quality of Farm Pond the class collected a variety of data. The class
Use a variety of tools and equipment to gather data (e.g., microscopes, thermometers, analog and digital meters, computers, spring scales, balances, metric rulers, graduated cylinders, stopwatches)