Solid Phase Peptide Synthesis And Solid-3 Synthesis Of A Primer

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In this essay, solid phase peptide synthesis and solid phase synthesis of a primer will be compared and contrast. Solid phase peptide synthesis is the production of peptides, whereas the solid phase synthesis of a primer involves the production of oligonucleotides. A brief summary of each will be discussed in this essay. With understanding the processes involves, there are two similarities and differences that will also be discussed in this essay.
In the solid phase peptide synthesis, a protected amino acid with a tert-Butyloxycarbonyl protecting group is anchored to a chlorinated reactive resin. Afterwards, the amino acid is deprotected with trifluoroacetic acid. After this is done, another protected amino activated with DCC is coupled to the last amino acid at the N-terminal. Whenever all of the amino acids are done being added, the peptide is released from the resin using hydrofluoric acid.
In the solid phase synthesis of primers, a ß-cyanoethyl (ßCE) group is added to the 3’-phosphoryl oxygen atom, while a dimethoxytrityl (DMT) protecting group is attached to the 5’-oxygen atom. Moreover, the 3’ amine group of the activated monomer is coupled to the 5’ hydroxyl group of a nucleotide base attached to resin, creating a phosphite trimester intermediate. Next, the molecule is oxidized with I2, adding a carbonyl oxygen atom to the phosphoryl group. Lastly, the molecule is deprotected with dichloroacetitc acid, which helps remove the DM, but leaves the ßCE intact.
To begin,
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