Essay on Sordaria Lab Report

The purpose of this experiment is to determine which stimuli sowbugs prefer more, moist or dark. However, to confirm that sowbugs prefer dark and moist environments we had to create an experiment to prove this. We proved sowbugs prefer dark environments by using a lamp to create a light environment and cardboard over half the petri dish to create the dark environment; the sowbugs chose the dark side. Then we proved sowbugs preferred moist environments over dry ones by creating a wet environment on one side and a dry environment on the other side of the petri dish. After our final experiment, we were able to determine that sowbugs prefer the dark stimuli over moist stimuli.

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

…offers many advantages, including a substantial body of research into aspects of its life cycle and genetics, a rapid life cycle allowing studies to be conducted in a timely manner, ease of isolating strains from the wild and grown them in the laboratory, and the fact that it produces fruiting bodies (perithecia) containing narrow, elongate asci with eight spores that are easily viewable under the microscope, and whose order reflects events that occur during meiosis (Burpee, Cyr, Hass, Ikis, Richter, Ward, & Woodward, 2015).

1. Describe the function of the following pieces of safety equipment and how each might be used: (10 points)

1) Apply the stain to your first unknown slide and examine it under the microscope.

|EMB Agar | |Distinguishes bacteria that ferment |Dark blue colonies with|E. coli and P. |

Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections, diarrhea, and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly, it is also the cause of many nosocomial infections due to its presence in water (1).

Observation: no bugs were found except small, black, gnats were all close to the ground.

The major storage polysaccharide in plants is starch. These molecules would be found in abundance in the stroma in the plant tubers where it is found as granules. Glucose is stored mainly in the form of starch granules, in plastids like chloroplasts and amyloplasts. Plant starch starts out as glucose, but glucose is very hard for plants to store, so it is converted to starch through polymerization. Amyoplasts turn the glucose into starch and move it to the stroma, and in tubers the stroma is a place to store the food (starch), and when plants need the energy in the starch, it converts the starch back into glucose.

The basis for this experiment was inspired by the evolution canyon in Israel that contained various species of Sordaria Fimicola (S. Fimicola) and more importantly had a tight ecological barrier. The canyon has two slopes that experience vastly different environmental conditions and thus expose organisms who inhabit them with bipolar pressures. Organisms living on the South Facing Slope (SFS) experience higher and more intense amounts of solar radiation, temperature, and drought. While Sordaria who inhabit the North Facing Slope (NFS) receive more cool and humid conditions. Sampled Sordaria from the two slope showed variances in cross-over frequency that occurs during Prophase I of Meiosis. This promising fact lead to the investigation of UV radiation of different types of Sordaria discussed later and promotes the idea that harsh environmental condition, like those experienced on the SFS, can cause variances in cross-over frequency. (Burpee et al., 2017)

The purpose of the “chi-square test” was to see if our data was in an acceptable range of a specific ratio listed above. The chi-square test took into account the expected deviations in the F2 offspring’s alleles.

Prior the conduction of this experiment, Sordaria of different ascus types (WT and T) were exposed to significant levels of radiation. There were three experimental groups for this experiment and crosses accordingly. Crosses were set up in containers by placing different Sordaria groups in alternating fourths of the mating agar. The Sordaria naturally grew outwards to the different adjacent Sordaria groups and inter-mated through meiosis. This provided a variety of different crosses throughout each experimental group. The squashes were prepared by obtaining a clear slide and placing a small amount of water in order to trap and spread out the perithecia. Once the individual perithecia were separated, they were busted by applying slight pressure with an eraser head, but done carefully enough not to pop the individual sacs. Frequencies were calculated by counting the number of

In order to accurately identify many fungi it is essential to observe the precise arrangement of the conidiophores and the way in which spores are produced (conidial ontogeny). One plate of nutrient agar commonly potato dextrose is recommended, however, I used nutrient agar as typical usage. Firstly, cut out an agar block (7 x 7 mm) small enough using a sterile blade to fit under a coverslip. Using a sterile straw to make a circle out of the fresh agar and fit under a glass slide. Flip the block up onto the new fresh plate and inoculate the four sides of the agar block with spores or mycelial fragments of the fungus to be grown. Place a flamed coverslip centrally upon the agar block then incubate the plate at 26oC

The following procedure dealt with a chromatogram. The materials needed are: a pencil, safety goggles, scissors, chromatography paper strip, capillary tube, spinach plant pigment extract, test tube, cork stopper, graduated cylinder, chromatography solvent (alternative isopropyl alcohol), metric ruler, stopwatch or clock with a secondhand, hook/fashioned paperclip, paper towels, test tube rack, and mortar and pestle. First we obtained a strip of chromatography paper and cut it so it would fit inside a test tube (with it barely touching the bottom of the tube). Also, when touching the strip, touch the sides only. Then we attached (firmly) the top of the strip to a hook (or fashioned paperclip at bottom of the cork stopper). Make sure it fits in the test tube. Next we used the pencil to draw a faint line across the strip two centimeters from the bottom tip of the strip. We placed the cork and strip in place, and we put a mark on the test tube one centimeter below the top of the stopper.

2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to