Spectrophotometer: Concentration and Standard Curve Essay

Bradford Assay on Unknown Concentrations of Proteins Taylor Coleman September 27, 2016 Lab Group 3 BIOL 1111: General Biology Lab Fall 2015 Section 107 Chad Perry Abstract Colorimetric assay is a process of determining a concentration of a solution based on absorbance of light. The purpose of this lab is to determine if the Bradford assay is an accurate way to determine an unknown concentration of two samples of protein. The Bradford assay is done by measuring wavelength of light passing

made that manufacturers had improperly labeled protein concentrations on their products. Concentrations of these three different protein-containing liquids, whole milk, soy milk, and a protein solution were determined using a Bradford dye reagent and a spectrophotometer to measure the absorbance of each. Absorbance of a set of standard known protein concentrations from a range of 0.125 mg/ml to 2.000 mg/ml were measured using the spectrophotometer at a set wavelength and this data was plotted. The equation

interesting as the main idea seemed to be the proper use and importance of a spectrophotometer but was completed in such a bizarre manner that I am unsure as to wether this was the original purpose or not. The first portion of the experiment seemed to be a practice 10 fold serial dilution using the chemical bromophenol blue, and was treated as a quantitative experiment. The second portion of the experiment used known concentrations , which was created though a second ten fold serial dilution with the chemical

In this investigation concentration of protein was to be found using the concentration of a known protein( already labeled) by measuring the concentration of protein in milk and the absorbance of light. It is hypothesized that the protein that will be tested will be the same as the already labeled one. The outcome from the lab shows that the protein that was used did not respond the same as the already labeled one known from the standard curve, therefore rejecting the hypothesis.The results prove

Discover a standard curve by using a known concentration of nitrate. Then finally utilize the standard curve to determine the concentration of the unknown solution. The first step in conducting the experiment is using an indicator brilliant blue dye to add to the concentrated protein solution. The solution will then turn reddish brown because of the high volume in the reagent is unstable. Therefore the reagent binds to the protein in the solution and becomes stable. Using a spectrophotometer, the equipment

sulfuric acid causes all non- reducing sugars to be converted to reducing sugars so that this method determines the total sugar present. This method is non- stoichemetric and so it is necessary to prepare a calibration curve using a series of standard known carbohydrate concentration (http://www.merriam-webster.com/dictionary/phenol-sulfuric acid method). Dilute Acid Hydrolysis is a process of hydrolyzing lignocellulosic materials by subjecting dried lignocellulosic material in a reactor to a catalyst

Investigation into haemoglobin levels, and the relationship between absorption and concentration. 551+146+85+71 = 853 Introduction 82+41+56+108+57+70+41+96 =551 Haematology, is a well-used important tool for diagnosing anaemia in patients, via a full blood count alongside a blood film. There are many parameters considered, from Haemoglobin levels, white blood counts and platelet counts to full white blood cell counts, also referred to as a full blood count ( FBC ) (Bain, 2006) ;(National Institute

To create the standard curve, the spectrophotometer had to be set up. The spectrometer was set to a wavelength of 580 nanometers, the wavelength of blue light. The spectrophotometer’s transmittance and absorbance were zeroed out. The transmittance was zeroed out by keeping the chamber empty and then setting the transmittance to 0, using the spectrophotometer knobs. The absorbance was zeroed out by placing the blank test tube, which consisted

Beer-Lambert Law which establishes a linear relationship between the concentration of a solution and the amount of light that the solution absorbs. Moreover, another objective of this experiment was to gain an understanding about the mechanical components of a spectrophotometer and to successfully use the device to measure absorbance or transmittance values. Lastly, another goal of this lab was to understand the concept of calibration curves and the process of interpolating data. Essentially, this lab utilized

QUANTITATIVE DETERMINATION OF COPPER (II) CONCENTRATION BY SPECTROPHOTOMETRY D.DEL PRADO1, J. BELANO1, M.MAHUSAY2,and M.FRANCISCO2 1 DEPARTMENT OF FOOD SCIENCE AND NUTRITION, COLLEGE OF HOME ECONOMICS 2INSTITUTE OF CHEMISTRY, COLLEGE OF SCIENCE UNIVERSITY OF THE PHLIPPINES, DILIMAN, QUEZON CITY 1101, PHILIPPINES DATE SUBMITTED: 12 MARCH 2013 DATE PERFORMED: 7 MARCH 2013 ------------------------------------------------- ------------------------------------------------- ABSTRACT -------------------------------------------------