Spectrophotometric Determination of Ascorbic Acid Concentration in an Unknown Solution

The number of moles of ascorbic acid neutralized in the experiment is given by multiplying the moles of NaOH used (0.00235128 mol) by the unit conversion coefficient (1 mol ascorbic acid/1 mol NaOH).

In this experiment redox-based titrimetric analysis was used to determine the amount of ascorbic acid (Vitamin C) contained in a serving of lemonade. This was done to confirm the 100 percent recommended daily allowance claimed on the package for a serving of Country Time Lemonade. The titration method used was adding a thyodene indicator to the analyte (lemonade solution) there is a visible colour change when the endpoint of the reaction between the titrant (KIO3) and the analyte occurs. The following reactions show how this titration method is viable. (Draw in reactions) KIO3 first reduced with iodide (I-) to generate iodine (I2 ) which then oxidized the ascorbic acid within the lemonade. Once all the ascorbic acid had reacted any iodine

Antioxidant rich foods are also extremely important. Antioxidants are found in other plant based foods, essentially those which contain large amounts of vitamin C and vitamin E. Foods such as blueberries, strawberries, lemons and limes fall into this category. Of course there are many others. Green tea is also very high in antioxidants and is a great drink to consume multiple times throughout the day.

The roles of antioxidants( Endogenous compounds) are to neutralize the excess of free radicals, to protect the cells against their toxic effects and to contribute in disease

Vitamin C - May help prevent cell damage and reduce risk for certain cancers,heart disease and other diseases.

Vitamin C is very essential to our bodies. Vitamin C helps the body to maintain its collective tissues. This also includes our bones, blood vessels, and skin. Vitamin C is scientifically known as L-ascorbic acid. The vitamin is a water-soluble vitamin that can be found in several fruits and vegetables. Vitamin C can also be found as a dietary supplement. It is essential for our bodies to have some source of vitamin C intake. “Vitamin C is required for the biosynthesis of collagen, L-carnitine, and certain neurotransmitters; vitamin C is also involved in protein metabolism. Collagen is an essential component of connective tissue, which plays a vital role in wound healing. Vitamin C is also an important physiological antioxidant [3] and has been shown to regenerate other antioxidants within the body, including alpha-tocopherol (vitamin E).” (1) The overall body intake for vitamin C runs to a high of 2g. Lower amounts of vitamin C can be found in areas such as eyes, pituitary gland, and

Planning My Experiment Problem: How do different temperatures affect the ascorbic acid content of orange juice? Purpose: In order to determine how the ascorbic acid content of pure orange juice is affected by different temperatures. Hypothesis: If the juices are heated, then they would contain less ascorbic acid than the juices that are chilled. Independent/Manipulated variable:

In the aeration samples, one flask was stationary and it was exposed to the atmosphere. The second sample was put in a regular flask, and placed in a shaking water bath. The third sample was placed in an irregular shaped flask, which was also placed in the quivering water bath. After treatment, all the samples were measured on the spectrophotometer, using the same procedure as the nutrient samples and temperature samples.

Glutathione and uric acid are antioxidants that are made by the cells. Glutathione performs most of its antioxidant activity within the cell, while uric acid does most of its antioxidant activity in the blood plasma. The antioxidants, which can be obtained from the diet, include vitamin C, vitamin E, carotenoids and selenium.

Astaxanthin (ASXT), the most powerful natural antioxidant found in nature, is a red pigment that has been attracting a considerable interest from several sectors of the scientific, biotechnological and commercial activity due to its noticeable and varied bio-functional properties of great relevance in human health and nutrition. Astaxanthin is ubiquitous in nature, especially in the marine environment [1], and it is a red pigment common to many marine animals, such as salmonids, shrimp, lobsters, and crayfish, contributing to the pinkish-red color of their flesh [2]. Because these animals are unable to synthesize astaxanthin de novo is must

The purpose of this experiment was to explain how colorimetry can be used to qualitatively detect cellular chemical components; to chemically differentiate between proteins, sugars, starches, and lipids; to identify the roles of molecular components in living systems; to comprehend the value of using a systematic approach to research; and to describe why hypotheses, controls, standards, and quality control are important in scientific research (Vorndam, 2002, p. 92).

Vitamin C is an organic compound that is also known as ascorbic acid. The molecular formula for Vitamin C is C6O8H6. This structure contains a 5-membered ring. Vitamin C contains 4 alcohol functional groups, an alkene, and an ester. Within the ring it contains one double bond, the carbon=carbon bond in the ring structure, this is an alkene functional group. The Vitamin C structure also contains an ester which helps form the five-membered ring.

Numerous scientific studies show that the people whose food intake is rich in antioxidants and who also take antioxidant supplements, have better chances to live longer, healthier and happier lives. Antioxidants are a family of vitamins, minerals, and other nutrients that may prevent or delay some types of cell damage. Thus, they might be beneficial in preventing diseases and contribute to the length and quality of human life. Hundreds of antioxidants are produced in the human body, but some necessary antioxidants must be obtained from the outer sources. But do they work separately in our bodies? Or antioxidants in our bodies can incorporate with each other in order to improve human lives. Some studies about antioxidants come up with a

Treatment with chloambucil in high dose is associated with toxicities to many organs.THE AIM of this study is to investigate the protective role of L-ascorbic acid for prevention of chlorambucil induced hepatic and renal toxicities in rats. METHODS: This study was conducted on 160 adult male albino rats divided into 8 equal groups . Group: I rats surved as negative control.Group II received L-ascorbic acid in an oral dose of 100mg/kg/day . Group III received chlorambucil in an oral dose of 0.2mg/kg/day for 5 consecutive days .Group IV received chlorambucil in the same dose for 10 days.Group V received chlorambucil in the same dose for 15 days.Group VI recieved chlorambucil and L-ascorbic acid in oral doses of 100mg/kg/day,0.2mg/kg/day respectively for 5 days. Group VII recieved chlorambucil and L-ascorbic acid in the same doses for 10 days.Group VIII recieved chlorambucil and L-ascorbic acid in the same doses for 15 days RESULTS:There was significant difference in mean values of serum ALT ,AST, bilirubin ,createnine, blood urea nitrogen and hepatic and renal GSH in groups III,IV and V compared to group I .Also, groups VI,VII and VIII showed significant difference in all studied parameters compared to group I and when groups VI,VII andVIII were compared with groups III,IV and V respectively ,

Two beakers were labeled ascorbic acid (C6H8O6) and iodine (I2). About 75.0 mL of standard ascorbic acid solution was measured in a graduated cylinder. It was then placed in the beaker labeled ascorbic solution. About 100.0 mL of iodine solution was measured using a graduated cylinder. The measured solution was then placed in the beaker labeled iodine. From the iodine previously gathered, about 55.0 mL was measured and placed into the burette. All of the solution did not fit into the burette. The burette was filled until the iodine solution was at the 0.0 mL mark.