Spectrophotometry : Results And Relevance

Spectroscopy is the study of light. A spectrophotometer is a machine used to determine the absorbance of light at any given wavelength. It does this by using a source of white light through a prism, which gives multiple wavelengths that can be individually focused (Ayyagari and Nigam, 2007). Substances are put into cuvettes that are glass or quartz containers that light can easily travel through. The light that is being focused travels through the substance gets absorbed by the

Spectrophotmetry is not only confined within the boundaries of chemistry. Its principles have several applications in different branches of science. In biochemistry, it can be used to determine enzyme-catalyzed reactions, whereas in clinical application, it is used to examine blood and tissues. This quantitative method of analysis can also be used in engineering, physics,etc.[2]

Light is a form of energy that behaves in some ways like waves. Light waves have a range of wavelengths. A wavelength is the distance between any point on one wave and the corresponding point on the next wave. Different wavelengths of light appear to us as different colors. Light that contains all wavelengths in the same proportions as sunlight appears white. See LIGHT.

Spectrometer – A spectrometer is a device which takes in light and then breaks down light into the various components of the electromagnetic spectrum.

6. A spectrophotometer would definitely be useful in experiments involving the measurement of concentration that produces color, since the color absorbance can be used to find concentration. One experiment involving a spectrophotometer could be finding the molarity of a blue Copper(II) sulfate solution. The wavelength could be set to red, and then the absorbance values can be correlated to the molarity of Copper(II)

This experiment was design to find the amount of copper in a penny using a visible spectroscopy. We achieved this by combining .89 to .93 of Cu(NO3)2*2.5H2O to a 25mL volumetric flask and filling the flask to about half full with deionized water, swirl, then fill to the line with more deionized water. After, fill a 25mL burret with Cu2+ stock, with this add .5, 1, 1.5, and 2 mL of the stock to four different volumetric flask, add 2mL of 15 M NH3 from the burret to each flask and swirl, then fill to the line with deionized water. First, calibrate the spectrophotometer then; enter samples of each of the four samples in the spectrophotometer to test the absorbency. For the penny stock solution, use a 500 mL filter flask with about 250 mL of 1 M NaOH, place a rubber stopper to the top and connect a inverted long stem funnel to it using tygon tubing, and place a 125 mL flask below it. Turn on the water aspirator, place the penny in the 125 mL flask, measure out 20-25 mL of 8 M nitric acid in a 50 mL graduated cylinder, and then slowly add it. It should take about 5-10 minutes for the penny to dissolve.

Conclusion: This lab shows how when heat or electricity is added, then the electrons move to an excited and move out and it also shows that the electromagnetic spectrum is quantized. 1a. How are wavelength and frequency related? Wavelength and frequency are inversely proportional or in other words, as one goes up, the other goes down, and when one goes down, the other goes up.

Electromagnetic radiation behaves both as particles (called photons) and as waves. Wavelength () and frequency () are related according to the equation

Spectrophotometry is a routine laboratory test that has the added advantage of being able to

In this experiment, paper chromatography was used to determine what pigments were present in spinach extract. From this experiment, we can see that four different types of pigments are present in the spinach extract used, the following are those pigments: chlorophyll a, chlorophyll b, beta carotene, and xanthophyll. The absorption and reflection of these pigments all revolve around the basis of the electromagnetic spectrum. The form of electromagnetic radiation is released as light and overall it is a type of energy that travels in waves. Going back to the spectrum itself, all the different types of electromagnetic radiation combine to form the electromagnetic spectrum, which tells us which colors can be absorbed and/or reflected. Each wave

Electromagnetic Radiation can help us in various ways to learn about matter in the universe. It helps us by determining the location of matter in the universe, planets for example. The waves that bounce back at a certain time can determine the distance between you and the planet. NASA and other people have come up with many ways of finding matter in the universe. There is a lot of ways to find matter in the universe but this is the most popular. The type of wave it sends back may be ragged or straight because of the surface that it is bouncing on. Radio waves and microwaves are used to observe interstellar clouds and track where the dark gas might be heading. Infrared waves that we can see from very powerful telescopes

Spectroscopy is a field of chemistry used to analyze chemicals in food and pharmaceuticals all the way to determining the age and compositions of the stars and galaxies (Santiago et al. 2015). For the lab we performed over the past two weeks we were using spectroscopy to test and understand the interactions of UV-visible radiation and organic molecules in commercial products such as food dyes. The spectroscopy is used in commercial products to find the exact concentrations of each dye, how much UV-light is absorbed correlates to how concentrated the dye is in the commercial product; or in our case how much concentrated dye is in each solution. We were give three dyes to perform the experiment they were red dye 3, yellow dye 5 and

Radiant energy released by certain electromagnetic processes is Electromagnetic Radiation. It is radiant energy that is all around us and takes many forms whether it be visible, such as, visible light, or invisible electromagnetic radiations, such as radio waves, infrared light, sunlight, and x-rays.

Although all electromagnetic radiation is light, our eyes can only perceive a small portion, known as the visible light spectrum. Other names for the visible light spectrum are known as the optical spectrum of light and the spectrum of white light. We can see only a little portion of the spectrum for the reason being that some parts of the visible spectrum contains wavelengths that are either too big or too small and energetic for the human eye to comprehend. The additional wavelengths and frequencies that are included in electromagnetic radiation and not in the visible light spectrum are basically invisible to the human

Visible light makes up a very small part of the full electromagnetic spectrum. Electromagnetic waves that are of higher energy than visible light (higher frequency, shorter wavelength) include ultraviolet light, X-rays, and gamma rays. Lower energy waves (lower frequency, longer wavelength) include infrared light, microwaves, and radio and television waves.