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Stainless Blue Battery

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Chamber Take the stainless steel wire and measure out a piece that is 21 cm. Make two. Bend the wires such that it hooks around the edge of the plastic box and is parallel to the width of the box. Figure 1. Each stainless steel wire is bent such that it can hook over the edge of the box Connect the five 9V batteries together so the positive terminal of one battery connects to the negative terminal of another battery. Once all five batteries are connected there should be positive and negative terminal open on opposite sides. Figure 3. Five 9V batteries are connected to form a battery pack Using the styrofoam, cut out a rectangular piece with dimensions of 18.5 cm by 9.5cm. Next, make a mark 1 cm down from the top of the length (the comb …show more content…

Figure 4. Styrofoam comb constructed to make wells in the gel solution (PC: Science Buddies) Solution Create buffer solution which consists of 1% solution of baking soda. To create the solution, mix 2 grams of sodium bicarbonate with 200 grams of bottled water. Stir well. Then take 100 mL of buffer solution, and mix in 0.5 gram of agarose, to create a 0.5% agarose gel solution. Stir well. Heat solution in microwave to dissolve the powder. Stir solution every 10 seconds. Remove solution from microwave after 90 seconds. Insert styrofoam comb in one end of the chamber, having a 3 cm separation from the end of the box. Gently pour the agarose solution into the chamber (make sure comb is only submerged 0.5 cm) Figure 5. Keep 5 millimeter gap from the bottom of the chamber to the comb (PC: Science Buddies) When gel solidifies, pour the remaining 100 mL of buffer solution over the gel. Carefully pull out the comb after the remaining buffer solution is poured over. Take the butter knife and slice through the gel, 1.5 cm from the edge. Using the butter knife, carefully cut a thin slice of the gel from the top and the bottom to make room for the

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