Starch Granules

4. There are other types of reagents used to determine what type of biomolecule a substance is. For example, copper ions present in Benedict’s reagent reacts with the free end of any reducing sugars, such as glucose, when heated. Originally blue in color, these copper ions are reduced by the sugar, and produce an orange-red colored precipitate. Alternatively, iodine-potassium iodide (IKI) may also be used when working with starch. IKI contains special tri-iodine ions which interact with the coiled structure of a starch

1. Benedict’s solution is added to white grape juice and heated. The color changes from blue to orange. Based on this result, what biological molecules are

0.0375 mg/ml Porcine Pancreatic Amylase Solution (amylase powder in 0.9% NaCl ), Iodine Solution; each solution were pipetted into each of the 5 test tubes with 5 ml of 1% starch. Each tube contained a 1% starch solution with a different pH. All tubes were at room temperature. Room temperature was 22C. 0.2 ml of porcine pancreatic amylase solution was then pipetted into each tube. A timer was started and every 3minutes the starch / amylase mixture were pipetted from each tube and pipetted into the spot plate for every sample tube, then the iodine solution were added to a spot plate cell for each sample. Iodine reacts with starch to change from yellow to deep blue /black in the presence of starch. A lightening of the blue/ black to a brown color will occur as less starch is present. Results were reported as (+) for presence of starch in the sample or (–) for the absence of starch. After every three minute increment had passed, these same

A final example of a polysaccharide is cellulose which is the main constituent of plant cell walls. It has adjacent chains of long, unbranched polymers of glucose which bond together to form micro fibrils. These layers are orientated in different directions and interwoven providing rigid cell wall with gaps in layers to provide permeability. The carbohydrate glucose is formed in the light-independent reaction which occurs in the stroma. Carbon dioxide combines with RuBP to form a highly unstable 6-carbon compound which splits in to two glycerate-3-phosphates which is then reduced to form two trios phosphate. This can either be recycled back to RuBP or go on to synthesise glucose. In conclusion, carbohydrates are essential for the storage of energy, structural support for example as the backbone of DNA and are an intermediate in respiration e.g. glyceraldehydes.

A cell, the building block of all living organisms, is composed of four fundamental biomolecules: proteins, carbohydrates, sugars and lipids. Proteins provide a vast amount of functions cells such as they serve as enzymes, provide structural support to cells, and act as antibodies. Reagents are used to spark a chemical reaction. The reagent used to detect protein traces in a substance is Biuret’s. Biuret’s will turn purple if proteins are present and blue if they are none. Biuret’s copper particles, have a charge of +2, are diminished to a charge of +1 when peptide bonds, which are in proteins, are present, creating the color change. Polysaccharides, which are carbohydrates, are most notably known to provide energy to the body, but they also help in breaking down fatty acids. Iodine is the reagent used to determine whether a substance has starch in it. The iodine/starch complex has energy levels that are only for retaining unmistakable light, giving the complex its extraordinarily dark black-blue shade. If there is no starch found, iodine will remain its natural yellowish-brownish color, but if starch is present, iodine will turn blue-black. Monosaccharides, which are sugars, like polysaccharides, provide the body with energy. To detect monosaccharides, the reagent, Benedict’s, is used. Benedict’s reagent is added to a test tube, then it is placed in

(Driskell 29) These simple carbohydrates are easily broken down, converted into glucose for the body and easily flow into the blood stream. The downside to this form of carbohydrates is that they are most common in the form of refined sugars or refined flours which retain very few vitamins after being processed. (“HealthStyle Fitness”) As the body uses this form of glucose a cycle begins which involves fluctuations of high and low blood sugar. This cycle works when blood sugar rises after the consumption of simple carbs and then as the blood sugar begins to drop the body demands more simple carbs to bring blood sugar levels back to up. The cycle that is formed essentially keeps the body from burning body fat because there is a continuous demand and consumption of simple carbohydrates. The second form of carbohydrates is that of complex carbohydrates which consist of polysaccharides, three or more monosaccharides. These complex carbs are found in the form of starch, fiber and glycogen (animal starch). Those complex carbohydrates consumed in the form of starch are found in plant foods like corn, wheat, potatoes, cereals, pastas, and beans. When they are broken down these carbs allow for the slow production and release of glucose into the blood stream and allowing for a consistent blood sugar level. (Guthrie 27) Dietary fiber is another form of complex carbs which are present in plant foods because fiber is

The purpose of this experiment is to exemplify how differences in molecular weight allow separation of polymers from their monomers. Methods of dialysis and gel filtration chromatography will be used to separate a glucose monomer from a starch polymer. Colorimetric glucose oxidase assay will be used to monitor the presence of glucose and a colorimetric iodine assay will be used to monitor the presence of starch in prepared solutions after separation

Carbohydrates are macromolecules which and contains carbon, hydrogen and oxygen (C,H,O). There are also two types of sugars, sweet soluble and starches which are non-soluble. Sugars can also be single or simple sugars.Carbohydrates can be found in a wide variety of foods such as pasta, wheat and grains, rice, potatoes, fruits and sugars. Carbohydrates are broken down into

Introduction: I know prior to doing this experiment that iodine mixed with starch creates a dark color and that most objects, organic and inorganic, naturally experience isotonic reactions.

This experiment consisted of setting up a control group of starch in various temperature and then placing both fungal amylases and bacterial amylases in a mixture of starch and placing the solution of amylase and starch in various temperatures of water. After a certain amount of time- different amount of time needs to be used in order to have reliable results- iodine is added in a well on spot plates, then two drops of the mixture of amylase-starch is added from each temperature used, by adding iodine into the plates the mixture will show how much starch was hydrolyzed, this is used to calculate the amount of

Arrowroot is made a from a tuber that was first used by the Caribbean Arawak people. It is an effective thickener as well as a useful alternative to wheat flour in biscuits, cakes and various baked goods. Cornstarch is made from the corn kernel's endosperm and was originally considered inedible but later became popular as a culinary thickener. How do these two starches compare to each other? What are their benefits and drawbacks? Below, we compare cornstarch and arrowroot.

1. 5 sucrose solutions were made of increasing molarity: 0.2 M, 0.4 M, 0.6 M, 0.8 M, 1.0M. 2. 50 mL of each unknown solution were poured into 5 separate cups. A slice of potato was placed into 5 equal cylinders. 3. The mass of the 5 potato cylinders were then recorded. 4. The cylinders were placed into the foam cups with solution and covered with plastic wrap. It is to be left overnight. 5. The room temperature was recorded in Celsius. 6. The cylinders are then to be removed from the cups and carefully blotted of any excess solution. 7. The mass of the potato cylinders were recorded afterwards.

In the first part of this laboratory experiment, a multi-colored leaf was removed from a Coleus plant that was in direct sunlight for several hours. The hypothesized results for which pigments were present and the results of an I2KI starch test were then recorded. A boiling alcohol bath was set

Hypothesis: If we decrease the level of pH in the enzyme Amylase, it will not be able to denature the carbohydrates in the potato starch solution after 10 drops because enzymes are very sensitive to pH levels and lowering it too much will compromise its ability to break them down.

Starch or amylum is a carbohydrate consisting of a large number of glucose units joined by glycosidic bonds. This polysaccharide is produced by all green plants as an energy store. It is the most common carbohydrate in the human diet and is contained in large amounts in such staple foods as potatoes, wheat, maize (corn), rice, and cassava.