Stc Case Study

Enzyme catalysis is dependant upon factors such as concentration of enzyme and substrate, temperature and pH. These factors determine the rate of reaction, and an increase in temperature or pH above the optimum will

Enzymes are biological catalysts that speed up chemical reactions, without being used up or changed. Catalase is a globular protein molecule that is found in all living cells. A globular protein is a protein with its molecules curled up into a 'ball' shape. All enzymes have an active site. This is where another molecule(s) can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme, a product is produced. Enzymes are specific to their substrate, because the shape of their active site will only fit the shape of their substrate. It is said that the substrate is complimentary to their substrate.

Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is

Question: How does changing enzyme concentration or temperature affect the reaction time of enzyme activity?

3) Adding less enzyme caused the reaction to proceed more slowly than when more enzyme was utilized.

The independent variable in this investigation is pH. Each individual enzyme has it’s own pH characteristic. This is because the hydrogen and ionic bonds between –NH2 and –COOH groups of the polypeptides that make up the enzyme, fix the exact arrangement of the active site of an enzyme. It is crucial to be aware of how even small changes in the

The motive of this lab is to attain a better understanding of enzyme activity by timing chemical reactions in certain temperatures and pH levels. Enzymes act as catalysts that help speed up reactions. Without these enzymes chemical reactions in metabolism would be backed up. There are two factors that affect an enzyme’s reaction rate: temperature and pH levels. In this label we will be testing different pH levels and temperatures to see which ones cause the most reactions.

Substrate concentration also affects the rate of reaction as the greater the substrate concentration the faster the rate of reaction and all the active sites are filled. At this point the rate of reaction can only be increased if you add more enzymes in to make more active sites available.

However, the rate of reaction only increases for a certain period of time until there is lesser substrate molecules than the enzyme molecules. The increase of enzyme concentration does not have effect if there are lesser substrate molecules than enzyme molecules initially.

The enzyme can change shape when bound by the substrate, which is triggered by the strong magnetic forces during binding. This change in shape aligns the two substrates in the positions in which they are connected by the hook-and-loop fastener (Figure 2). This simulation shows important features of the induced-fit model: sub- strate binding induces a change in the shape of the enzyme, which brings about the catalysis of the substrates into the product. This simulation helps tackle the misconceptions that the substrates bind with the enzyme in a lock-and-key manner and that it is the sub- strate, rather than the enzyme, that undergoes induced fit (College Board,

Over the course of being at STLCC I have written project in a research paper format. Some were for educational purposes, and some that had a major impact on my personal life, and most importantly based on history that not many have heard about. For my project I will like to use a previously completed project. I have a very busy schedule, and I would like to put all the effort possible into this project. I have two ideas in mind one is about “Comic Books” what are they? How are we supposed to read them? Why are they so popular? How they improve grammar skills and etc. Second idea is about history and people that are living in America at the present moment. People known as “Ahiska Turks” are people that are living in America at the present moment, but they have a wide history behind everything these people went through; the

Chronic Myeloid Leukaemia is a hematopoietic proliferative disorder associated with a specific defect in the gene (Ciesla 2007, pp. 189). The gene defect is caused due to the translocation of the genetic material between chromosome 9 and chromosome 22 (t9: 22). The translocation prompts to the development of a hybrid gene BCR-ALB in the Philadelphia chromosome, or Ph1. Therefore, this fusion gene mutation affects the maturation and differentiation of the haematopoietic cells (Ciesla 2007, pp. 189). However the introduction of a BCR-ALB particular tyrosine kinase inhibitor, Imatinib has significantly enhanced the survival rate of the patient with chronic myeloid leukaemia (Hochhaus, et al.2015). This essay will briefly describe the diagnosis, treatment and future strategies for chronic myeloid leukaemia.

The Linweaver-Burk plot can therefore be used to make inferences about Km and Vmax as the normal functionality of an enzyme is suppressed by specific

Each enzyme is very specific and can only catalyze a certain reaction. The specific reaction catalyzed by an enzyme depends on the molecular structure and shape of a small area of the enzyme’s surface called the active site. The active site an attract and hold only its specific molecules. The target molecule that the enzyme attracts and acts upon is called the substrate. The substrate and the active site of the molecule must fit together very closely. Sometimes the enzyme changes its shape slightly to bring about the necessary fit.

Enzymes are natural catalysts that work from the ability to increase the rate of reaction by decreasing the activation energy of a reaction. (Blanco, Blanco 2017) An enzyme can do this 10^8- to 10^10 fold, sometimes even 10^15 fold. (Malacinsk, Freifelder 1998) The substrate will momentarily bind with the enzyme making the enzyme-substrate complex, of which the shape of the substrate is complimentary to the shape of the active site on the enzyme it is binding with. There are two main theories as to how an enzymes and substrates interact, the lock-and-key model and induced fit theory. The lock-and-key model suggests that the enzyme has a specific shape that fits the substrate and only that substrate. The induced fit theory says the active site and substrate are able to change shape or distort for the reaction to take place with (Cooper,