Stem Cell Lab Report

To test the blood, we first dropped two drops of blood into two spots in a micro-well plate, then we added the appropriate serum to both and mixed with a small stick. One side was labeled A, for the A Anti-Serum, and one was labeled B. If the A blood clotted it meant that the A antigen was found, and likewise with B. If both clotted the blood type was AB, and if neither clotted the blood was type O. Some errors made

A cover slip is added and filter paper is used to absorb any excess liquid. We then drew our observation by observing cells under 40X then 100X and then high power 400x using a microscope. In addition, for a slide 3, we placed 1 drop of 10% Sodium Chloride solution onto the slide with the drop of blood. Then we added a cover slip, observed cells under a microscope and observation is made. Lastly, for a slide 4, 1 drop of distilled water is placed onto the slide with the drop of blood. Due to excess liquid, filter paper is used to remove the unnecessary liquid. Then the slide is placed under 40X then 100X and then higher 400X power in a microscope to investigate the diffusion through living

diH20 acted as a negative control in the experiment, all cells, both in intrafollicular zone and in interfollicular zone stained purple. Cells stained brown in the negative control indicate non-specific staining.

15 cell lines were acquired for the purpose of this experiment. Process of subcellular compartment fractionation was performed on these cell lines, this included complete cells, nucleus and cytosol (seen in

Rat blood was tested in different solutions with different concentrations to test if hemolysis of the red blood cells would occur. This was tested by using a index card behind the test tubes to see if the lines were clearly visible enough through the solution meaning which could concur hemolysis. Urea and water were the only solutions permeable to the red blood cell's membrane. The red blood cell's lysed causing

HMECs and MDA-MV-231 cells will be plated 1 x 103 cells/well in 96-well plates and cultured overnight (16-18 h). On the following day, test compounds will be diluted in growth medium to the desired final concentration and then added to the cells. After 3-4 days of incubation, CellTiter-Gloc Reagent will be added and mixed. The plates will then be incubated for 10 minutes to stabilize the luminescent signal then read on a luminometer plate reader at 560 nm. GI50 values can be determined from semilog plots of percent of control versus concentration (14, 36). The normal HMBCs will serve as a control cell line to gauge the effect of the compounds in normal tissue. This homogenous assay “add-mix-measure” format results in cell lysis and generation

To begin this portion of the total blood cell count a finger prick was completed. In order to do this gloves were put on the participant’s hands and the finger being used to prick was thoroughly cleaned with an alcohol pad (allowed to air dry). Next a sterile lancet was used to stick the cleaned finger until blood appeared. Holding the hand down, two capillary tubes were filled at least two thirds of the way full with the participant’s blood. After the capillary tubes were filled they were sealed by pressing the clean end into the clay pad. Both capillary tubes were then placed onto the centrifuge for three minutes to spin, (which separated the elements of blood). The group then used a hematocrit card reader to measure the percentage of red blood cells. To do this the bottom of the capillary tube was placed on the scale at 0 and the top of the plasma at 100. The value at the top of the red blood cell portion indicates the percentage of whole red blood cells. Materials for collecting the white blood cell differential information included three clean glass slides, a sharp, wrights stain, a buffer for wrights stain, bibulous paper, a stacking rack, and a microscope. This experiment also requires a finger prick, if more blood was needed then it was performed again as previously stated. A small drop of blood was placed on two of the three clean slides. The third clean slide was used to smear the blood on each of the slides containing the drop. The slides were to be held flat while the third slide was used to push the blood down the slide in a thin layer. After a thin blood smear was created on both slides the blood was allowed to air dry for the staining process. Following the slides drying, they were placed on a stacking rack with the blood

Mice were anesthetized in end of challenge and were bled via the brachial plexus to collect the blood samples that were used to estimate IgE production. Ice-cold PBS (0.5 mL) was instilled twice into the lungs, and BALF was collected. Total cell counts were performed using a hemocytometer. The fluid recovered from each sample was centrifuged (4 °C, 3,000 rpm, 10 min) to pellet the cells, and the supernatant was kept at −70 °C until it was used for cytokine measurements. The cell pellets were resuspended in PBS to stain and count the total number of cells using the Wright-Giemsa staining method. At least 200 cells were counted per

The purpose of this experiment was to determine the effects of tonicity on a cell membrane using red blood cells, potato strips and three unknown solutions (A, B, C). First three slides were prepared containing RBC’s and unknown solutions A, B and C. A control slide was prepared only using RBC’s. After observing each slide under the microscope it was determined that unknown solution A was hypertonic because the RBC appeared to have shrunk. The RBC in unknown solution B appeared to be swollen, therefor, the tonicity of unknown solution B was hypotonic. Unknown solution C showed no change to the RBC shape, it was suggested that unknown solution C was isotonic. To confirm the tonicity

Table 1 provides the total count and frequency percentage of five different types of leukocytes (white blood cells), commonly found in a peripheral blood smear. This data was collected from the participant referenced in the report. An abundance of lymphocytes and neutrophils were discovered, collectively making up 81% of the blood’s white blood cell components; Lymphocytes found at 30% of white blood cells and Neutrophils at 51%. Monocytes were somewhat common, however, were only 16% of the blood’s leukocyte components. Eosinophils were rare in the blood smear, only found to be 3% of the total white blood cell count. Basophils were not found in any field of view during the exercise.

The purchased primary culture of the canine aortic endothelial cells (1 mL) will arrive frozen. The arrived sample will be thawed and put into the culture. While taking precautions, the ampule of canine aortic endothelial cells will be taken out of the liquid nitrogen storage and it will then be placed into the water bath (without submerging the cap) set 37◦C for about 1 to 2 minutes or as soon as ice melts. The ample of the mammalian cells will be wiped down with the paper towel moistened with 70% ethanol and 30% water. The minimum essential medium 1X (MEM) media will be pre-warmed to 37◦C in the water bath. Then, 11 mL of the MEM media will be transferred to a sterile T-75 flask through pipetting. The 1 mL of the canine’s cells will be pipetted

Phosphate-Buffered Saline (PBS) was used to clean the excess media in the cell and to maintain the osmolarity of the cells. In the cell and tissue culture, PBS was needed to remove the serum of media for trypsin to detach the cells from the flask . The salt ions in the PBS was isotonic to the cell because of equal amount of salt ions inside the cells. The cell will burst if the solution contain a few of salt ion and would be shrink if too much contain of salt ions. Therefore, the amount of salt ions and osmolarity in PBS must be correct to keep the cells in an isotonic state.

This experiment is performed to estimate the number of erythrocytes in the sample, by counting of the number of cells in one of the centre grid chamber of haemocytometer. Blood is

The number of red blood cells in all three experimental groups was consistent with literature values reported for the black-6 wild type mouse from which the (NZW x BXSB)F1 hybrid is derived, which verified our expectation that these hybrid mice do not express an anemic phenotype. In contrast, there was a relative decrease in the number of blood leukocytes in the control mice relative to WT mice, suggesting that white blood cells are recruited to infiltrate the host tissue from the blood stream, after which they eventually promote inflammation. Consistent with our expectations, the number of white blood cells was higher in ABX and RS treated mice relative to the controls, suggesting that in these mice the autoimmune response was less severe

When RBCs are suspended in an isotonic solution, nothing should be observed as there will be no net water movement between the RBCs and the solution. Thus, when 200 µL of blood was added to 10mL isotonic saline (0,154 M NaCl), voltage of 0V is recorded. When RBCs are suspended in a hypertonic solution, for instance, 0.4 M NaCl, RBCs shrunk due to decrease in cell volume as water diffused out of the cells by osmosis. The protein concentration within the cells become greater and more light is scattered and a negative