Stem Cells and Parkinson's Disease Essay

2606 Words11 Pages
The goal of this paper is to compare the utility of adult, embryonic and induced pluripotent stem cells (iPSCs) to treat Parkinson’s disease. As such several things will be assessed, dosage of stemcells, improvement in motor function, in combination with the presence of α-synuclein proteins and cell survival. To give a short overview of the steps that will be taken to complete the study. Obtaining stem cells, whether adult, embryonic or induced, shall be done using healthy mouse models and after ethical approval has been gained. The process to derive them will be detailed below, however they are also purchasable commercially with the benefit of being well studied and accompanied by a detailed analysis of properties, however with a…show more content…
And analysis will one subtype shows greater vulnerability to α-synuclein proteins. Culturing of mouse embryonic stem cells: Many protocols have been utilized to culture mESC’s. Lin and Talbot have written a chapter on the culturing of both mouse and human embryonic stem cells. The culturing is done using 2 sets of cells, mouse embryonic fibroblasts (mEFs) to provide a feeder layer, and the culturing of the actual mouse embryonic stem cells (mESCs). Ensure reagents are at 37 degrees Celsius to prevent temperature shock to cells. mEF medium contains the following: Dulbecco’s Modified Eagle’s Medium (DMEM), L-glutamine, penicillin/streptomycin and knockout SR-medium (preferable to FBS, since it can have changes in consistency between batches, also can promote differentiate embryonic stem cells). Coat a T75-cm2 culture flasks with 0.2% gelatin to provide better adhesion surface for mEFs. mEFs can either be purchased commercially or obtained in the following manner. Pregnant mice are sacrificed between 12,5 and 13,5 days after mating. Embryos are removed from uterus and placed in sterile PBS. Head and internal organs are removed from embryo. In fresh PBS 1mm sections are cut then transferred into trypsin/EDTA. Stir cells for 40 minutes, add DNase if it looks viscous and clumpy. Proceed by adding mEF medium, then strain the solution
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