Steps in Banana Shoot-Tip Culture

When looking at DNA extraction, the key steps for isolating DNA from a plant cell or animal cell is to pass through the protective barriers of each cell – whether it is a cell wall and cell membrane or only the cell membrane for an animal cell. In order to extract DNA from a banana, the first step required is to place half of it in a small resealable plastic bag without air bubbles, and then using your fingers mash the banana until no visible chunks. Next, using a graduated cylinder measure 10 mL of the 0.1% NaCl solution, and add it to the bag – mix thoroughly. Then the next step is with a graduated cylinder measure 3 mL of detergent solution (dH2O), add it to the bag and mix gently, so as not to form bubbles. After this, set up a retort stand with the gas tube using a retort clamp, strain the banana mixture through a filter paper, and collect the filtrate in a gas tube for approximately

John Soluri 's Banana Cultures: Agriculture, Consumption and Environmental Change in Honduras and the United States, (Which for spatial and repetitive purposes, I will refer to as Banana Cultures for the remainder of the paper), introduces the reader to a world of corporate greed, consumption, and environmental change using the history of the common, everyday fruit, the banana. He explores the various political occurrences, health problems, and changes in mass media through the rise of the consumption of the banana in the United States, and around the globe.

First, before starting my experiment I did some research on the growth of lima beans, so that I could start the base of the project. According to research done at the University of

To produce different plants, genetic cross-breeding is performed, as well as using different techniques for growing the plant.

The third step that was taken was germinating the seeds. Two sets of paper towels were used to germinate the

The experiment was successfully accomplished in classroom where the Brassica Rapa were grown under suitable conditions. The experiment began by developing a watering system to the plants using the idea of capillary action. The watering system was made up of a plastic reservoir filled with a certain amount of water and a water mat placed on the top of the plastic reservoir. This design was helpful in making sure that the plants are receiving water constantly. Also, one piece of copper sulfate was addedinto the reservoir to avoid any algal growth. Two Styrofoam quads with four cells each were used for planting the seeds of Brassica Rapa. The cells were numbered from one to eight and a wick was placed in each cell in a way that allowed water access from the water mat through the capillary action. Each cell was then half way filled with soil and two fertilizers were placed in

Root development from the seed developing downwards to give more sub roots then they develop into a hair like substance it reamains connected as the stem is growing up and after that it breaks the dirt and creates takes off.

The tobacco plant like many plants contain a cell callus. A cell callus contains somatic undifferentiated cells and can be used to differentiate into specialized tissues of the tobacco plant, or any plant used, by being induced with the addition of different types of hormones, such as cytokinin and auxin. Cytokinin and auxin are mostly used in plant tissue culture simultaneously to provoke the formation of a plantlet or callus. There is a common use of Kinetin in plant tissue culture since when added it will promote cell division to initiate shoot tissues from calluses of the plant. Kinetin is a type of cytokinin hormone. In regards to auxin related hormones, Indole-3-acetic acid (IAA) is also commonly used since it promotes the initiation of roots for the root tissue of the plant. In this

Material: Wayfaringtree Viburnum seeds, dissecting microscope, scalpel, forceps Wayfaringtree Viburnum (Viburnum lantana) was used in this lab to conduct the growth of an embryo rescue on hormone free (0/0 WPM). The seeds that were provided first be treated with a concentration of sulfuric acid (H2SO4) for about twenty-five minutes. This process will both sterilize and soften to seeds for dissecting into

Before proper tests can be performed, the most important aspect to laboratory procedures are aseptic techniques. These techniques are crucial to ensure that no contamination occurs during the culturing process (1). Although the exact steps may vary depending on the media and type of transfer, the main components of the aseptic techniques

each to be plated on 0.4 g potato dextrose agar (PDA). The PDA (10.26% potato extract, 51.28% glucose, 38.46% agar, 35.5366 Nm cycloheximide) uses potatoes as a source of nitrogen, potassium, and glucose as the carbon source, while cycloheximide is used to inhibit fungal growth. Of each dilution 0.1 ml was plated on PDA and spread with the hockey stick method. Each plate was then labelled with the dilution factor and placed in the incubator at 37°C for 24-48 hours dependent upon growth. A seventh plate was created with soil from wet sample, plated in the same manner on PDA.

Much can be learned from studying an organisms DNA. The first step to doing this is extracting DNA from cells. In this experiment, you will isolate DNA from the cells of fruit. Materials (1) 10 mL Graduated Cylinder(2) 100 mL Beakers15 cm Cheesecloth1 Resealable Bag1 Rubber Band (Large. Contains latex pleasewear gloves when handling if you have a latex allergy).Standing Test TubeWooden Stir StickFresh, Soft Fruit (e.g., Grapes, Strawberries, Banana, etc.) ScissorsDNA Extraction SolutionIce Cold EthanolYou Must ProvideContains sodium chloride, detergent and waterFor ice cold ethanol, store in the freezer 60 minutes before use. Procedure If you have not done so, prepare the ethanol by placing it in a freezer for approximately 60 minutes.

This data shows a strange outcome, in the hypothesis; it says that “If acid is introduced to the seed during germination, then the roots will not grow as long as the seeds that are given water”. This statement proves to be untrue, because the roots grew longer with stronger acid than weaker acid, and in some, cases, grew better with strong acid than it did in water. This may be true because of the acid growth theory. The acid growth theory states that auxins cause the elongation of stem cells by promoting wall loosening. It was determined that this wall loosening is caused by hydrogen ions. This idea and subsequent supporting data gave rise to the acid growth theory, which states that when exposed to auxins, susceptible cells excrete protons into the wall at an enhanced rate, which in turn decreases the pH in the wall. The lowered wall pH then activates the wall loosening process which is essentially doing the same thing as the auxin hormone.

Orange Juice Materials: Three oranges were used for this experiment to obtain a decent amount of juice. Each orange was cut in half and juiced according to steps 1 and 2 of Appendix I of the Laboratory Manual for Biology 201 (Denniston, Wimmers, & Hemm 2015). The juice from the first orange and one half of the second orange was strained into a small beaker following

In this technique, the plants are cultivated in a narrow horizontal pipes wherein a thin layer of nutrient rich solution is flows and circulates through it continuously (TECA, 2015). This system is only suitable for small-sized plants because large plants that have big roots will clog the pipes. The plants are placed in a net pot within the holes of the pipe. In this technique, a mechanical solid separator and biofilter needs to be added. a biofilter is necessary because that is where the nitrification process will