Study Measuring Oxygen Production Of Spinacea Oleracea Using An Oxygen Electrode

Measuring the Rate Oxygen Production using an Oxygen Electrode Chamber in Photosynthesis of Spinacia oleracea with Varying Light Intensities

Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.

This lab was conducted to discover which level of light (measured in lux) would make Spinacia oleracea leaf disks float the fastest. Lux (lx) is latin for light, and is the unit of measurement for light in the International System of Units (Rouse 2011). Light plays a key role in the process of photosynthesis and in this we are essentially measuring the rate at which Spinacia oleracea leaf disks convert a bicarbonate solution into oxygen. The oxygen then would bubble up, allowing

Introduction: Photosynthesis can be defined as a solar powered process that removes atmospheric carbon dioxide and transforms it into oxygen and carbohydrates (Harris-Haller 2014). Photosynthesis can be considered to be the most important biochemical process on Earth because it helps plants to grow its roots, leaves, and fruits, and plants serve as autotrophs which are crucial to the food chain on earth. Several factors determine the process of photosynthesis. Light is one these factors and is the main subject of this experiment. The intensity of light is a property of light that is important for photosynthesis to occur. Brighter light causes more light to touch the surface of the plant which increases the rate of photosynthesis (Speer 1997). This is why there is a tendency of higher rates of photosynthesis in climates with a lot of sunlight than areas that primarily do not get as much sunlight. Light wavelength is also a property of

The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.

The purpose of this experiment was to investigate the effects of light intensity on the rate of photosynthesis in a Moneywort plant. By observing the plant in distilled water mixed with sodium bicarbonate, different light bulbs were targeted onto the plant. The measurement of the amount of bubbles present on the plant during the trial of the experiment enabled us to identify the comparisons between the activity of the light and the process of photosynthesis.

-Measuring the pH of a solution (such as in the lab we had) could also help determine the rate of photosynthesis. You would need a much more specific pH meter, but generally, if pH goes down, the level of CO2 is higher, meaning more cellular respiration. Higher pH means there’s less CO2, so more photosynthesis.

The rate of photosynthesis can be determined different ways. Because oxygen is a product of photosynthesis and the Elodea plant is submerged in water, the oxygen is released in bubbles that rise to the surface of the water in the beaker. In this experiment, the rate of photosynthesis for each degree of light intensity can be measured by counting the number of bubbles released every 30 seconds for five minutes at each distance. The rate is the number of bubbles released per minute.

The process of photosynthesis, by which light energy is used to convert inorganic compounds into organic substances with the release of oxygen, may be the most important biological event sustaining life (Keir et al. 2017). In the light-dependent reactions, the chloroplasts of a plant use the pigment chlorophyll to convert light energy into chemical energy. This energy is used to split water and produce oxygen (Eller et al. 2015). The energy is later used in the light independent reactions, where carbon dioxide (CO2) undergoes carbon fixation with the aid of enzyme rubisco, because it catalyses both carboxylation and oxygenation reactions and most of responses of photosynthesis to light, CO2, and temperature (John Evans 2013).

Think fast. Think green. What comes to mind when you hear the word photosynthesis? Take a short quiz to see how much you know!

The intention of this experiment is to determine the effects of pH on the rate of photosynthesis in living leaves. Photosynthesis is a process by which plants convert light energy captured from the sun into chemical energy which they use to perform various plant functions. During the photosynthesis process, light, carbon dioxide, and water react to produce products: sugar and oxygen. The equation for photosynthesis is:

The purpose was to determine how levels of light affect photosynthesis. This was done by placing portions of a green plant in a bromothymol blue solution and observing the colour changes that occurred. It was determined that plants exposed to light produce far more oxygen than those placed in a dark environment. This confirms the hypothesis that oxygen is necessary for continued photosynthesis. Grade 12 University Biology, SB14U, Rockland District High School Wednesday May 11th, 2016

However, the photosynthetic process can be affected by different environmental factors. In the following experiment, we tested the effects that the light intensity, light wavelength and pigment had on photosynthesis. The action spectrum of photosynthesis shows which wavelength of light is the most effective using only one line. The absorption spectrum plots how much light is absorbed at different wavelengths by one or more different pigment types. Organisms have different optimal functional ranges, so it is for our benefit to discover the conditions that this process works best. If the environmental conditions of light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will increase with average light intensity and under the wavelengths of white light which will correspond to the absorption spectrum of the pigments. The null hypothesis to this would be; if the environmental conditions light intensity, light wavelength and pigment type are changed, then the rate of photosynthesis will decrease with average light intensity and under the white light which will correspond to the absorption spectrum of the pigments.

In this lab, varying wavelengths were used to test how light affects photosynthesis and respiration as a whole. The absorbance of lights from 380 nm to 720 nm of chlorophyll pigment from the Elodea sample

     Photosynthesis has a two-stage performance before plants produce the two products they are known to produce. These stages are Photosystem I and II. Photosystem II is dependant on light reactions for energy which causes the electrons to be react and be transferred to Photosystem II. The electrons are transported through the Photosystem II electron transport system, however some energy is used to drive ATP synthesis. Meanwhile, light is being absorbed by the Photosystem I, which causes the electrons to react. This process sends the electrons to the Photosystem I transport system where some energy is released as electrons travel through the electron transport system and is captured as NADPH. When this process is completed oxygen is released from the plant and glucose has been