Study of Oxygen Consumption to Measure Energy Metabolism in Mammals

And finally into test tube 3, I pipetted 1.0 ml turnip extract and 4.0 ml of water. The contents of test tube 1 was poured into a spectrometer tube and labeled it “B” for blank. “B” tube was now inserted it into the spectrometer. An adjustment to the control knob was made to zero the absorbance reading on the spectrometer since one cannot continue the experiment if the spectrometer is not zeroed. A combination of two people and a stop watch was now needed to not only record the time of the reaction, but to mix the reagents in a precise and accurate manner. As my partner recorded the time, I quickly poured tube 3 into tube 2. I then poured tube 2 into the experiment spectrometer tube labeled “E” and inserted it into the spectrometer. A partner then recorded the absorbance reading for every 20 seconds for a total of 120 seconds. After the experiment, a brown color in the tube should be observed to indicate the reaction was carried out. Using sterile techniques, any excess liquid left was disposed

The content of this section depends to a large extend on the nature of the experiment. Topics here should include a section labelled:

Added to the respirometers will be KOH(potassium hydroxide) to selectively remove any carbon dioxide produced with the consumption of oxygen. The three respirometers will be submerged in each bath and allowed to equilibrate. After the equilibration period the respirometers are submerged in the baths taking reading at 5 minute intervals, recording the amount of oxygen consumed in ml on the pipette attached to each respirometer.

direct calorimetry. Furthermore, the amount of oxygen in the chamber reveals the amount of cellular respiration of the organism. While also, test the effects of decreasing oxygen, and later increasing the heat on the metabolic rate of goldfish. I hypothesize that an increase in temperature will increase their metabolic rate

The respirometers will be prepared using two different types of cotton balls; nonabsorbent cotton and absorbing cotton. The absorbent cotton will be placed at the bottom of the respirometer and added with 15% KOH. Then, the nonabsorbent cotton

To perform this test, a tube of broth rich with glucose is acquired. In this tube is phenol red, a pH indicator. Initially, the tube appeared pink in color, indicating a normal pH level. Next, a sample of unknown #44 is introduced into this medium using the aseptic technique, and this is allowed to sit for several days. If the organism is able to ferment glucose, the pH in the medium would decrease and cause the phenol red to exhibit a yellow color. In addition to the straw color, gas can also be produced and trapped inside the Durham tube placed in the medium. This production of acid and gas is a direct result of the fermentation of glucose, as seen with unknown

The first experiment begun by filling a 600-ml beaker, almost to the top, with water. Next, a 10-ml graduated cylinder was filled to the top with water. Once water was added to the beaker and graduated cylinder, a thumb was placed over the top of the graduated cylinder. This would ensure that no water was let out and no bubbles were let into the graduated cylinder. Next, it was turned upside down and fully submerged into the beaker. Then, a U-shaped glass tube was attained. The short end of the glass tube was placed into the beaker with the tip inside of the graduated cylinder. Next, a 50-ml Erlenmeyer flask was received. After, 10-ml of substrate concentration and 10-ml of catalase/buffer solution were placed into the flask. A rubber stopper was then placed on the opening of the flask. After adding these, the flask was held at the neck and spun softly

Our team was given the temperature of 37 degrees celsius. For this experiment we will be looking for any bubbles and measuring air change in a submerged cylinder. Our hypothesis stated: “At 37 degrees celsius the enzyme would have affected the catalase

My partner and I choose glucose, colchicine, cold temperature (0 degrees Celsius), and warm temperature (37 degrees Celsius) as the addition factors. The tetrahymena were exposed exposed to their condition for ten minutes before the India ink was placed in their microcentrifuge. The tertrahymena were fixed on a slide every ten minutes after the ink was placed in the microcentrifuge. In this experiment there are two controls. There is positive control and a negative control. The untreated sample is tetrahymena that did not have any other conditions besides the ink. Then before the sample was placed on the slide they were fixed. The treated sample was tetrahymen mixed with

Please complete the entire experiment as instructed in the lab manual except for any modifications noted below. Fill out the

The purpose of the Cellular Respiration lab was to identify if cellular respiration is sped up due to exercise. To conduct the experiment each student filled a beaker with 15 ml of water and three drops of bromothymol blue. Next each student blew into the beaker through a straw until the water mix turns yellow. When carbon dioxide is added too bromothymol blue it changes to a yellow color. Each student times how long it takes for the water to turn yellow, records the data, and then reset the lab. Subsequently the student would exercise for a total of five minutes and then repeating the lab, again recording how long it takes for the water mix to turn yellow.

The listed materials were taken to a workstation and placed on a blue tray. Water was poured into the zip plastic bag. The red cabbage leaves were torn apart and crushed. They were then placed into the bag containing water. Pressure was then applied to the bag. The 8 cups were then placed on a piece of paper on the tray. The purple tinted water from the bag was then poured into the 5 clear plastic cups. The cream of tartar was then added to one of the 3 small cups. The vinegar was then added to the second small cup. The laundry detergent was then added to the last small cup. A toothpick was then dipped into the cream of tartar and then dipped into one of the 4 clear plastic cups. Observations were recorded. A separate toothpick was then dipped into the laundry detergent and then dipped into a different clear plastic cup than the last one. Observations were recorded. The final toothpick was then dipped

All systems must have homeostasis to maintain stability and to survive. Homeostasis is so important because it can allow an animal to adapt to a changing environment. The body attempts to maintain a constant level of physical output to achieve homeostasis. However, it can only work within its limits, where extreme conditions can disable the negative feedback mechanism.

First, the iodine test was preformed. Two grams of the unknown mixture were put into two different divots. A drop of iodine was put into one divot. Since the mixture turned blue-black, the powder was corn starch and the vinegar test was not necessary. Then, the flame test was preformed. The unknown mixture was put into a vial which was then filled with water. The vial was shaken until the mixture dissolved. Once it was fully dissolved, the mixture was poured through a paper filter into a test tube in order to remove the corn starch. A platinum rod was dipped into the test tube. The platinum rod was then placed over a lit bunsen burner. The color of the flame was

(This experiment is aimed to use 10 Artemia, however, 7 to 13 Artemias are able to achieve the goal in this experiment.). Then seal the cuvette under water and make sure no air bubble is inside the cuvette. After that, put the cuvette into the temperature controlled water bath for ten minutes. After 10 minutes, take the cuvette to the oxygen meter to measure the oxygen concentration by holding the end of the end of the fibre-optic cable squarely on to the senor spot from the outside of the cuvette until the concentration has been shown on screen and record it down. Then return the Artemia to the same incubation bath and repeat this procedure every 5 minutes and measure it for 4 to 5 times. After the process above, we have to find out the total length of the Artemias in the cuvette. To find the total length of the Artemia, use the pipette to move the Artemia out of the cuvette and settle them into a watch glass and measure the length of the Artemias by ruler. At last but not least, put those high energy intake Artemia back into the sink and repeat the experiment instead of those low intake Artemia. On the other hand, to find the difference of activity of the high and low energy intake Artemia, those Artemia will be tracked by the software named, the Tracker and the Tracker are able to determine the velocity of the Artemia under different treatment for 5 replicates.