The control trial for the fermentation rate experiment will be performed the same as outlined in the procedure for ‘Fermentation Investigation Week #1’ in the Alverno College CH213 Laboratory Manual, Fall 2016. In the second trial, the concentration of the sucrose in the reaction mixture will be changed from 6% to 12%. Prior to conducting our modified experiment, we will first conduct the original experiment with 6% sucrose that was done in week 1. To obtain 12% of sucrose we will use 10.00 mL of the prepared 6% (m/v) sucrose solution and we will then measure out 0.6 grams of a solid sucrose. Once we measure out the 6.0 grams of sucrose we will dissolve that into the 10.00mL of the prepared 6% (m/v) sucrose. This will allow us to obtain
Measured 21.4 (g) of sucrose, C¬12H22O11 to the nearest milligram using a scoopula and an electronic scale. 21.4 (g) of sucrose is the total mass needed for all five trials. The mass of sucrose was based on previous molar concentration calculations. 4. Measured 250 (mL) of distilled water to the nearest microliter graduation to ensure equivalency using
The control in the experiment is distilled water, as it does not contain sucrose solution.
Higher levels of solution should produce higher levels of product. The independent variable for the control group data and the experiment data is the yeast concentration. The dependent variable for the control group data and the experiment data is how much oxygen is produced. The Constant for the control group data and the experiment data is time and amount of hydrogen peroxide. The products of the experiment will increase if the levels of reactants increase. Denatured yeast may cause change in the reaction of the experiment. For all trials of the control group, the concentration of yeast is 6ml. For the experiment data, the yeast concentration varies from 8mL, 10 mL, 12 mL, 14 mL, and 16 mL. The temperature may cause change in the reaction of the combination of yeast and hydrogen peroxide
If the hypothesis is correct, the masses after we place the tubing samples in water will be higher than the masses before. We will see the greatest % increase in mass in the highest concentration of sucrose (1.0 M). The % increase will get lower as concentration decreases. All of the samples should have some increase in mass because we are placing them in distilled water. Based on the known solutions, the data should yield a mathematical model relating concentration and % increase, which we can use to find the concentration of the unknown solution.
PH can affect the way fermentation occurs due to the irregularity of the acidity or alkalinity within the glucose solution. This is an enzyme-based reaction that is susceptible to pH. The aim of this experiment was to determine how pH affects the yeast fermentation rate by performing the experiment numerous times with a different pH of glucose solution which included pH 3, 5, 7, 9, 11. The hypothesis was ‘If the pH is lower than the neutral point then the fermentation reaction will occur faster?’ The experiment conducted was to measure the amount of C02 produced by the yeast going into fermentation, however varying the pH of glucose solution by using different pHs . To test this every 5 minutes the volume of gas in the test tube was observed and recorded until a period of 30 minutes had been. The end results
The research question asks how varying sucrose concentrations affect the rate of anaerobic cell respiration in yeast, measured in CO2 production. The rate of anaerobic respiration will be determined by measuring the rate of CO2 production by the yeast cells.
Table 3: Experimental Design-Addition Of Sucrose: Tube 1 and 2 are the negative control A and positive control B. Tube 4 was the experimental as sucrose was added instead of glucose to test the amount of fermentation. Tube 3 was the positive control to compare the experimental (tube
Linear graph of the average amount of carbon dioxide produced (PPM) versus the different molar concentrations of surcrose (mol). The data shown on the graph was proven by the hypothesis, ultimately representing how increases in sucrose concentrations (mol) will produce more carbon dioxide (PPM) during yeast fermentation. Concentrations increased by 0.25 (mol) until 1.0 (mol) along with the measurement of carbon dioxide (PPM) produced for a solution with 0.0 (mol) of sucrose. Error bars were calculated using the standard deviation values to represent how spread apart the experiment’s results are.
3.Measure and add 0.5g, 1.0g, and 1.5g of sucrose into 3 of the test tubes. Do not add sucrose into the 4th test tube because this will be the control. Lightly shake the test tube to mix the contents together.
In order to test the predictions of the hypotonic, hypertonic, and isotonic hypothesis for the solution made during the study, four samples of sucrose were taken and placed into two different beakers each containing a different concentration. Beaker 1 is 250- mL and contained 150-mL of 10% sucrose with dialysis tubing A, while beaker 2 (a large bowl) contained 1% sucrose, with dialysis tubing B, C, and D. Tubing A contained 10-mL with 1% sucrose. Tubing B
In part 2 of the lab, we tested the effect of the enzyme invertase on dH2O (the control), sucrose, and
To start our experiment, we created our control that was filled with water heated to sixty degrees Fahrenheit, then added a yeast packet to the water and mixed the solution. Next, we acquired one mole of sucrose, glucose, and fructose. The independent variables in our experiment included sucrose, glucose, and fructose, while the
Anaerobic fermentation is a process that produces energy after glycolysis occurs if oxygen is not present or available. There are two types of anaerobic fermentation: alcoholic fermentation and lactic acid fermentation. Alcoholic fermentation is a process that converts sugars (pyruvic acid) into ethanol and carbon dioxide. This process occurs in the cytoplasm of yeast (fungus) cells and allows bread to rise and bubbles to form in champagne when introduced to specific temperatures. The purpose of this lab was to determine the effect of temperature on the rate of alcoholic fermentation. The hypothesis was that a higher temperature will allow the rate of fermentation to occur faster. The independent variable was the temperature the yeast was exposed to, and the dependent variable was the rate of fermentation. The experimental group was the test tube introduced to the room temperature beaker (Beaker C). The control group were the test tubes that were introduced to the hot and cold beakers (Beaker A and B). The constants in this experiment were the experiment took place,
Fermentation is a metabolic pathway that produce ATP molecules under anaerobic conditions (only undergoes glycolysis), NAD+ is used directly in glycolysis to form ATP molecules, which is not as efficient as cellular respiration because only 2ATP molecules are formed during the glycolysis. One type of fermentation is alcohol fermentation, it produces pyruvate molecules made by glycolysis and the yeast will break it down to give off carbon dioxide, the reactant is glucose and the byproducts are ethanol and carbon dioxide. In this lab, the purpose is to measure whether the changes of
1. 5 sucrose solutions were made of increasing molarity: 0.2 M, 0.4 M, 0.6 M, 0.8 M, 1.0M. 2. 50 mL of each unknown solution were poured into 5 separate cups. A slice of potato was placed into 5 equal cylinders. 3. The mass of the 5 potato cylinders were then recorded. 4. The cylinders were placed into the foam cups with solution and covered with plastic wrap. It is to be left overnight. 5. The room temperature was recorded in Celsius. 6. The cylinders are then to be removed from the cups and carefully blotted of any excess solution. 7. The mass of the potato cylinders were recorded afterwards.