Sulfolobus Acidocaldarius Report

A mixed culture of two unknown bacteria was provided by the instructor. The methods used for

After precisely conducting the experiment and tabulating the results, data for Paraquat toxicity upon P. vulgaris plants can be interpreted over several different parameters. The parameters by which Paraquat toxicity was examined within this experiment involve visual observations, x-ray diffraction, chlorophyll concentrations, protein concentrations, and lastly malondialdehyde (MDA) concentrations on a per mg of protein basis. As stated before Paraquat is very widely used herbicide known to produce superoxide anions leading to chloroplast membrane damage and ultimately a variety of adverse effects upon the host organism, in this case P. vulgaris (Chia et al., 1982).

In this experiment, we were given a sample of an unknown bacterium and asked to classify it based on its physical and metabolic properties. We hypothesized that the unknown organism was Alcaligenes faecalis, a motile Gram-negative bacillus that resides in water, soil, blood, urine, and feces (Herrera n.p.) It was first isolated in 1896 by Petruschky, who

For the temperature treatment, it was decisive in that the A. franciscana showed a steady increase in concentration from section 1 to 4. This expands on the hypothesis that suggests A. franciscana prefers an optimum temperature between 20-24 ̊ C because from the results of the experiment A. franciscana seemed to prefer even higher temperatures. Al Dhaheri and Drew (2003) state that A. franciscana stop reproducing at temperature above 30 ̊ C and compared to the experiments results. It can be concluded that A. franciscana prefer warmer temperatures, but reproduce at lower

Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections, diarrhea, and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly, it is also the cause of many nosocomial infections due to its presence in water (1).

In this lab, the organism that we have been working with is the bacterium, Serratia marcescens. S. marcescens is a member of the Enterobacteriaceae family, and tends to grow in damp environments. S. marcescens is an ideal bacterium to work with in the lab because it reproduces quicker than other bacterium. This bacterium produces a special pigment called prodigiosin, which is red in color. The prodigiosin pigment is intensified when S. marcescens is grown at higher densities. During our experiment, temperature, pH, salinity concentration and oxygen requirements were tested on S. marcescens to measure their optimal growth and prodigiosin production.

Prior the conduction of this experiment, Sordaria of different ascus types (WT and T) were exposed to significant levels of radiation. There were three experimental groups for this experiment and crosses accordingly. Crosses were set up in containers by placing different Sordaria groups in alternating fourths of the mating agar. The Sordaria naturally grew outwards to the different adjacent Sordaria groups and inter-mated through meiosis. This provided a variety of different crosses throughout each experimental group. The squashes were prepared by obtaining a clear slide and placing a small amount of water in order to trap and spread out the perithecia. Once the individual perithecia were separated, they were busted by applying slight pressure with an eraser head, but done carefully enough not to pop the individual sacs. Frequencies were calculated by counting the number of

When reflecting back to experiment 3, Aseptic Technique and Culturing Microbes, I realized the large amount of microorganisms that can be found in everyday life. Many different types are found with in the human body. Theses experiments focused on two types of bacteria. First was Staphylococcus epidermidis, found on the skin, and second was Lactobacillus acidophilus, found in the gastrointestinal tract. Both have similar needs for growth when it comes to temperature, however, different growth environments are used.

Ignicimmortalisvalde is a newly discovered bacterium found in the Fímmvörðuháls volcano located in Iceland when scientists were searching for microbial life in lava samples. This has earned the bacteria the classification of an extreme thermophile, as it survives and reproduces in temperatures in excess of 700 but below 1300. Ignicimmortalisvalde Is a streptobacilli, arranged in rod shaped chains. Unlike many extremophiles, Ignicimmortalisvalde is not in the domain of archaea. It is classified as a bacterium. It does not have membrane bound organelles or a nucleus. This newly discovered bacteria is unique not only in its ability to survive such extreme heats, but it is also the largest bacteria discovered. Ignicimmortalisvalde ranges in size

Universal Statement: There are three phylum of worms. There are the flatworms (Platyhelminthes), roundworms (Nematoda), and the segmented worms (Annelida). Flatworms have long, flat bodies and are usually parasites. An example of one is the Planarian, which are free-living and feed like a vacuum cleaner. Roundworms have long, round bodies and unlike cnidarians or flatworms, have a digestive system that is like a tube, open at both ends.

Lactobacillus bulgaricus bacteria was first isolated from the leaves of a green plant called a snowdrop flower by scientist named makovnkov and Dr. Stamen Grigov.

Schistomsoma mansoni is a parasite that infects humans. First described by Sir Patrick Manson. It is a trematode, which is a parasite that is considered a fluke. It is an internal parasite of mollusks and humans. This parasite is the cause of the disease known as schistosomiasis. During the life cycle, the parasite has two different hosts that help its survival. The single intermediate host is the freshwater snail; this is where the parasite goes through many asexual reproductive stages. Then on to a definitive host, a human, where the parasite undergoes sexual reproduction. The parasite is long and slim like worm with a funnel shaped oral sucker with a second pediculate sucker. The outer layer of the parasite

Life on this planet began with microorganisms. Through millions of years microorganisms have found ways to successfully adapt and survive. These adaptations have created a wide biodiversity, allowing them to basically populate in all places. Why are these microbes so important? Because they shape the history of our world. Some microbes can be deathly to humans while some others are favorable, for example, bacteria that lives in the gut of both humans and animals and helps during the process of digestion (Alfred Brown & Heidi Smith, 2006). Understanding these interactions help scientists to find ways to protect humans from potential deathly pathogens. In order to observe microbes, microscope proficiency and microorganisms’ identification are crucial skills in a microbiology lab. During this laboratory session, samples of environmental and human organisms were inoculated into two different rich media and incubated to their according temperature. After this, appropriate use and calibration of the microscope was performed. Lastly, morphology and size of different species of bacteria, algae, fungi and protozoan were recorded.

The purpose of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli, Pseudomonas fluorescens, Enterococcus faecalis, Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5, 25, 37, 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species. After one week they were observed for distinguishable changes by the turbidity showing an indication of bacterial growth, or the clarity an indication of no survival.

Live Lactobacillus casei Shirota strain, cultured and tested in our laboratory, is added to the tank. The temperature of the tank is then reduced until the contents are at 37°C (body temperature). The solution is allowed to ferment in the tank for 6-9 days or until the numbers of Lactobacillus casei bacteria reach their ideal concentration.