Sult2b1b Enzyme Lab Report

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The sulfating activity of the recombinant human SULT2B1b allozymes will be assayed by using PAP [35S] as a sulfate donor. The standard assay mixture final volume is 20µL and consist of 50 mM HEPES buffer at pH 7.0, 1mM DTT, 14 mM PAP [35S], and the substrate. The essay will be performed on different exogenous and endogenous substrates (see table 6). The controls for this experiment will be prepared with either water or dimethyl sulfoxide (DMSO) replacing substrate. The reaction will be initiated by adding 2µl of the enzyme. The reaction will be allowed to continue for 10 minutes in water bath at 37°C then the reaction will be terminated by placing the reaction tube on the heating block source for three minutes. Then to remove the participate,
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