Summary Of Daphnia's Heart Rate

After completing the experiment we found that when we gave the Daphnia caffeine the heartbeat rate did show an increase. However, we also found that alcohol also increased the number of times the heart beat. Even though we performed all of the experiments very carefully, we cannot be certain that the effect we saw was due to the drugs. Perhaps the change in heartbeat rate is caused by

In the first experiment, the heart rate of Daphnia at different concentrations of Nyquil® was analyzed. Nyquil® was added in 1:1, 2:1, 3:1, and 1:0 concentrations which caused a +16, +36, +68, and +108 BPM increase, compared to the control. On the other hand, all Daphnia died at high concentrations of ethanol, and the only time Daphnia survived was when the concentration of ethanol was diluted almost 80%. And with that dilution, the Daphnia’s HR was 28BMP, 152 less than normal heart rate of

For each test solution and base HR, the daphnia was submerged for ten-minutes to allow the chemicals to take effect, for daphnia are ectotherms and their body adapts to the surrounding environment. The data in Table 1 and Graph 1 answer the question of whether a daphnia, when exposed two depressants, i.e. melatonin and ethanol, will result in an average heart rate (HR) lower in comparison to the two depressants being administered individually. Over the three trials conducted for each of the test solution for daphnia 1, it can be concluded that 10% ethanol administration resulted in almost 2.6 fold, that is approximately 60%, decrease in HR. Therefore, ethanol had a depressant effect on average BPM, similar to Corotto's findings. (2010) Also, since the first daphnia died after

Then using a disposable pipette we placed two drops of room temperature water (21 degrees) on top of the Daphnia. Then placing it careful under the microscope with a for 15 seconds using a tally counter and clicked away how many heart beats we observed and did this three times for three trials, in between the times would give the daphnia a two minute recovery period. We then would process the trials information and calculate an average. After the information calculated we then  multiplied our results by four to see the average heart beats per minute with room temperature water. We gave the daphnia five minute to recover then went on to proceed with two drops of hot water (40 degrees celsius) placed on top of the Daphnia using the pipette. Again three trials for 15 second using the tally clicker following after a two minute break. The same followed for the cold water (0 degree celsius) placing two drops on top of the Daphnia administered by pipette. In between the transitional water temperature trials we then cleaned up the excess water before placing new drops of water by gently soaking up the water with kimwipes. After five minutes we tallied the heartbeats of the Daphnia before administering the epinephrine. Then again gently gave two doses of epinephrine using a new pipette. Then after we observed and tallying the heart beats before administering the epinephrine, three times again recording our results then averaging it. Then we placed two drops of the epinephrine on top of the daphnia, tallying the heartbeats and recording and calculating our trial results. Then after two minutes we observed and tallied the effects after the epinephrine had been used.  Each trial again consisted of observation under the microscope, tallying and observing the behavior internally and externally of the

The tachycardia is the “faster than normal heart rate at rest” (mayoclinic, 2015). The bradycardia is “the slower than normal heart rate” (mayoclinic, 2015). In the lab procedure, the students had to perform certain criteria to elevate or decline the HR of the Daphnia Magna’s and record their data collections in the Supplemental Lab Manual (SLM) table on pg. 42. Each

5. Establish the resting heart rate of the Daphnia. Count the amount of beats for ten seconds then multiply that number by six to convert it to beats/min.

To begin the experiment, we took a single Daphnia magna from the unused tank to run control tests with distilled water on the untampered heart rate of the species. The species was placed on a concave slide with a new drop of water on it. It was given 2 minutes of recovery time to adjust to its new environment; then, the heart rate was studied by intervals of 15 seconds to find beats per minute by multiplying the number of beats in 15 seconds by 4. The Daphnia magna was studied under a light microscope with 40x total magnification. After each round of data collection, the water was absorbed with a Kimwipe so a new water drop could be placed on the species. The same Daphnia magna was used for six rounds of control experiments. After

In my experiment as the concentration of sugar increased the heart rate of the Daphnia decreased. The cause of this may have been to the possibility of having a defectuos daphnia. My prediction was that the more sugar concentration added the highest the Daphnia heart rate was going to be. It did affect my organism but not in a positive way if not in a negative way, by decreasing the heart rate of the organism.

This can also be related to Daphnia in the same way. Therefore I predict that as the concentration of alcohol surrounding the Daphnia increases the greater the percentage decrease of heartbeat. ABSTRACT -------- The aim of the investigation was to find out the effect of alcohol on the heart rate of Daphnia.

The purpose of this lab was to determine the normal heart rate of a Daphnia Magna and decipher the different effects that various substances had on it. A Daphnia Magna is a species of water fleas and can be located in the Northern United Sates against the coastline of the Atlantic in rocky pools. The water flea’s habitat consists of rivers and streams, temporary pools, lakes and ponds, and brackish water. The Daphnia Magna range from two to five millimeters in length and are shaped like a kidney bean (Elenbaas, Molly). Relating to this lab experiment we learned in class that the normal heart rate is measured anywhere between 60 and 100 beats per minute (BPM). If your resting BPM is measured at a level above the number 100 it is known as Tachycardia. This term indicates that your heart level has exceeded the normal range. Also if your heart rate is indicated below 60 then it is called Bradycardia, which means the heart is beating slower than normal. When your heart rate is affected by a substance in the body it is called a Chronotropic agent. When the heart rate decreases because of a substance or chemical it is called a negative Chronotropic and when it is affected oppositely by increasing it is known as a positive Chronotropic agent. In this experiment we added many different substances to the slide on which the water flea was placed to calculate the increase or decrease in its heart rate due to the ingredients in the substances. The first substance used was

This experiment is to see The Affect of Caffeine on Daphnia. Daphnia is also known as a water flea. It is a distant relative of crabs and shrimps. (Marshall Cavendish, 2002) Daphnia have a hard external skeleton with jointed appendages and limbs. The head contains a large central eye which is made of two eyes joined together. Daphnia has two pairs of antennae which are used for swimming. Each daphnia has five pairs of limbs. These limbs lie in a certain space under the body bounded by the carapace. (Marshall Cavendish, 2002) A carapace is a hard upper shell or chitinous outer covering on the back of some animals. The daphnia is well preserved because of its shells which are composed of chitin. The usual size of this microorganism is about 0.2-0.3 mm; they look like flat disks. Their bodies are divided into three parts – head, thorax, and abdomen. The head is typically dome-shaped with five pairs of appendages. Among these five are two pairs of antennae; there is a small pair and a larger pair. The smaller pair of antennae serves as a sensory function and the larger one is used for swimming. The other three appendages’ purpose is to secure food they intake. (Michael Hutchins, 2003) The thorax holds four to six pairs of legs that are used for gathering food, filtering water, or grasping mates. There are over 400 species of daphnia and they are distributed worldwide. Daphnia uses their thoracic legs to produce a constant current of water; this allows them to filter food

Three Daphnia magna were placed in a petri dish with a small amount of spring water. Each Daphnia magna was measured separately. The Daphnia magna was placed on a slide and their control heart rate was measured for 15 seconds under the microscope and multiplied by four. Then, it was placed in the epinephrine solution for two minutes.The Daphnia magna was placed back onto the slide with the spring water and the heart rate was measured again for 15 seconds and later multiplied by four to observe the effect of epinephrine on the heart rate. They were then placed into a different petri dish of spring water after their experimental heart rate was recorded. All the heart rate values were placed into a Microsoft Excel document. The controlled variables included the temperature of the water, the time subjected to the epinephrine solution, and the time used for measuring the control heart rate and the epinephrine heart

The effects associated with alcohol are produced by the ethanol in the alcohol. The severity of these effects is reflected by the concentration of alcohol in an individual’s blood, which is dictated by the amount of alcohol ingested, the volume of blood, the individual’s metabolism, and amount of time since ingestion. In large doses, alcohol acts as a depressant of the central nervous-system. A blood alcohol level of 0.1% affects some of the motor areas of the brain associated with speech, balance and manual dexterity. A blood alcohol level of 0.2% depresses all motor functions and the area concerned with emotions is depressed. At a blood alcohol level of 0.45% the entire section of the brain that handles perception is depressed and the individual becomes comatose. At a blood alcohol level of 0.7% the parts of the brain that control the heartbeat and breathing are depressed and the individual

Alcohol is a diuretic. Diuretics are medicines which cause the removal of water from body. They are able to achieve this by increasing the amount or volume of urine your kidneys produce. The amount of water which is absorbed from the loop of Hénle is greatly reduced as the concentration of Na+ remains in the filtrate, this prevents the water being drawn into the renal medulla. Normally in the case of diuretic drugs, this would decrease the total blood volume in the body, giving your heart less work and reduce blood pressure, making diuretics a choice drug for heart failure and high blood pressure. Alcohol reduces the production rate of vasopressin, which also acts on the kidneys, decreasing the amount of water reabsorbed into the body. All

In the above mentioned studies, alcohol was found to