Synthesis and release of FSH and LH in pituitary cells are the principal role of GnRH receptor [19]. GnRH effects are largely mediated by ERK and NFAT, where both have been reported to be involved in various biological responses [20, 21]. In resting cells, ERK is characteristically cytoplasmic due to its association with MEK and other cytoplasmic anchors [22, 23]. Upon stimulation ERK is disassociated from MEK and changes in the structure of docking motifs are occurred. These alterations facilitate the passing of ERK into the nucleus via interactions with nuclear pore complex proteins, where ERK can alter gene expression and regulate various transcription factors [24].
Uniquely, type I mammalian GnRHR does not undergo rapid
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The auto phosphorylation of EGFR by EGF causes the association of Grb2 with the SH2 domain that recruits SOS-1 which is ultimately activating Raf and ERK signalling [30]. Here, ERK kinetics was broadly similar in the three cell lines examined here following stimulation by EGF, it was rapid and transient (Fig. 10F, 11F and 12F), and this could be due to the dynamics of its receptor, as upon EGF activation the EGFR undergoes rapid internalisation and degradation, thereby terminating ERK activation. Hydrolysis of GTP to GDP terminates Ras activation, and although Ras proteins have low GTPase activity, the response was transient. This may be due to negative feedback or other regulatory proteins acting as GTPase-activating proteins being activated by Ras, which in turn accelerates the hydrolysis of GTP to GDP and subsequently prevents prolonged Ras stimulation signalling [31].
As mentioned earlier, GnRHR signals mainly via ERK and NFAT, therefore monitoring these two key aspects are essential during pulsatile and sustained stimulation. In Chapter 3, NFAT-EFP translocation assay was used as a downstream readout for cytoplasmic Ca2+ in LßT2 and HeLa cells. Here, the work has extended further to MCF-7 cells. (Fig.10, 11 and 12). Overall, in these cell lines, sustained stimulation with GnRH resulted in sustained NFAT-EFP activation, with slower
A majority of exocrine pancreatic cancer patients exhibit a mutation in the proto-oncogene K-RAS (A.M. Lowy et al. 2008) and mutations in gene p16. K-RAS is a member of the RAS family of GTP-binding proteins; they’re essential in the role of cell proliferation, differentiation, and survival (Campbell et al. 1998; Malumbres and Barbacid 2003). Several studies have been able to identify the point mutation in K-RAS that are almost exclusively seen in pancreatic cancer. Point mutations at codon 12 (from CGT to GAT or GTT,) result in substitution of glycine with aspartate, valine, or arginine leading to an overactive K-RAS pathway. Once activated, K-RAS recruits and activates necessary proteins for the production of growth
There are numerous neurological changes occurring in our body as a response to fear. Specifically, two pathways are activated: the sympathetic nervous system and the hypothalamic- pituitary- adrenal pathway. In a similar way, both pathways commence by receiving messages from the hypothalamus, an area of the brain responsible for many functions in the body, such as activating the autonomic nervous system, controlling different organs, and managing physiological functions. If the hypothalamus emits messages that we are facing a threatening situation, it will release different chemicals to either the sympathetic nervous system and the hypothalamic- pituitary- adrenal pathway. This sympathetic nervous system are “nerve fibers of the autonomic
Advance downstream, changes in the tumor silencers TSC1 and TSC2 hyper initiate motioning by mTORC1 (Laplante and Sabatini 2012). This is a critical focus of P13K-Aktsignaling. Additionally, the Ras-ERK pathway is enacted by transformations in Ras, or its downstream target Raf, that reason constitutive initiation of these proteins or by inactivation of GTPase-actuating proteins (Holes, for example, NF1, that empower the hydrolysis of GTP bound to Ras, which prompt its inactivation (Cichowski,2001).
Different GRK subtypes combined with the action of -arrestins can contribute the endocytosis of Group I mGluRs in an agonist-dependent manner; however, the results are inconsistent (reviewed in Refs: Iacovelli 2013, Kim 2008). Both GRK2 and GRK4 induce the agonist-induced internalization of mGluR1a, which appears to require GRK2-mediated phosphorylation of the S869-V893 region of mGluR1a when expressed in HEK cells (Iacovelli 2003, Sallese 2000, Mundell 2003). These agonist-induced internalization processes are -arrestin 1/2 and dynamin-dependent (Mundell 2001, 2002, 2003). -arrestin 1 appears to be important in mGluR1 endocytosis; however, the agonist-stimulated internalization of mGluR1a is observed only when -arrestin 1 is co-expressed with either GRK2 or GRK5 in HEK cells. GRK2, GRK5, -arrestin 1, or -arrestin2 individually has no significant effect on the internalization of mGluR1a (Dale 2001). -arrestin 1, but not -arrestin 2, selectively interacts with mGluR1a and is redistributed to the same
Cell growth, migration, proliferation and mRNA levels of YAP regulated genes were tested using LPA treated cells that had YAP/TAZ knockdown. Cell growth, migration, proliferation and mRNA levels of YAP regulated genes were all significantly repressed compared to the controls suggesting that LPA acts through YAP/TAZ. Similar to YAP, TAZ was shown to localize in the nuclear fractions of cells in immunofluorescence experiments. MST 1/2 and Lats 1/2 levels were tested in response to LPA treatment. LPA was shown to inhibit Lats 1/2 but not MST 1/2 in immunoblot experiments. Then to test how LPA and S1P were modulating YAP/TAZ immunoblotting experiments were utilized. LPA and S1P were found to modulate YAP/TAZ through G-protein-coupled receptor signaling and Rho GTPases. More specifically, G12/13 receptors appeared to be most involved while Gq/11 receptors were only slightly involved in dephosphorylating YAP/TAZ. On the other hand, Gs receptors had the opposite effect, phosphorylating and inhibiting
The experiments that were completed previously offered a comprehensive understanding of how rGFP was induced, expressed, and purified. To outline, Ni2+-agarose affinity chromatography was done to separate the protein of interest through a strong affinity to the His-6 tag in the rGFP to the column. The Bradford assay is where the estimation of the amount protein of the samples was done. Then the SDS-PAGe gel showed an estimation of the molecular weight and purity of samples. This was important in identifying the protein. Finally developed a Western Blot, confirming the presence of rGFP through band
Testosterone, a key element in the genetic makeup of all human beings. The driving hormone controlled from the pituitary gland. This hormone dictates the sex drive of the male human being. However, this hormone also leads people to believe that males should act and be a certain way. The concept of hegemonic masculinity, where hegemonic is defined as ruling or dominant in a social class, and masculinity is defined as possession of the qualities traditionally associated with men (Morettini, 2016). It originated with concepts such as masculine fundamentalism, which was a form of promoting dominance of a man in a household of married men (Frances & Ester, 2013, p. 143). In today’s contemporary society, hegemonic masculinity is a key problem.
From a molecular perspective, hyperforin has a multi-directional mechanism of action. It acts on ligand-gated (GABA, NMDA, and AMPA receptors) (29, 30) and voltage-gated channels (Ca2+, K+, and Na+) (29, 31). In contrast to blockade of ion transport through the plasma membrane, hyperforin can increase inward Ca2+ currents. These processes are dose-dependent and may involve different cellular events. In vitro studies have shown that hyperforin increases intracellular Ca2+ levels by the activation of the non-selective canonical transient receptor potential 6 channel (TRPC6) or by releasing Ca2+ from the mitochondria (32–34). As has been previously shown, hyperforin activates intracellular signaling pathways leading to changes in intracellular Ca2+ levels. Leuner et al. showed that hyperforin increased the
Calcium measurements were made with a ratio imaging system (In Cyt Im2; Intracellular Imaging, Cincinnati, OH) using wavelengths of 340 and 380 nm and an emission wavelength of 505 nm. At least 5 cells were selected in each experimental condition, and experiments were repeated in at least three separate animals. The goblet cells were incubated in an inhibitor before RvD1 10-8 M or RvE1 10-9 M was added. The cholinergic agonist carbachol (CCh) (purchased from) was used as a positive control, as its pathways are known. The phospholipase C (PLC) inhibitor, U73122 (purchased from), and the control, U73343, were added 15 minutes before Rvs or CCh stimulation. 2-aminoethoxydiphenylborane (2-APB) (Tocris Biosciense, Bristol, UK) is an Inositol triphosphate (IP3) receptor antagonist. It was added 10 minutes before stimulation. The calmodulin-dependent protein kinase II inhibitor, Kn93 (purchased from), was added 30 minutes before stimulation. The control was Kn92. 1-butanol (purchased from), a phospholipase D (PLD)
Peptides and protein hormones like: (vasopressin, thyroid-stimulating hormone (TSH), and angiotensin) and neurotransmitters like GABA bind to G protein-coupled receptors (GPCRs).
One of the most important receptors in cell signalling is the trimeric (sinq kniga 596)G protein coupled receptor (GPCR). That is a receptor which has a surface part and an intracellular part (composed of 3 subunits a;b;y). GPCR pathway leads to the activation of another pathway group ERK (extracellular signal regulated kinases). This complex pathway is also regulated by phosphorylation (sinq kniga cell biology).
They also play an essential part in homeostatic metabolism by controlling activities and processes at distal sites in the body. The nuclear hormone receptor (NHR) is an example of a nuclear receptor for small-molecule chemical messengers. The NHR superfamily includes receptors for thyroid and steroid hormones, retinoids and vitamin D, as well as different orphan receptors for unknown ligands13. The current effort in NHR drug discovery is directed towards the improvement of current NHR drugs or screening currently unexploited
The reoccurrence of GBM is attributed to the activation of the epidermal growth factor receptor (EGFR), a receptor that if expression is misregulated leads to tumor development, as well as its progression, metastatic spread, and a decreased survival for the individual. In many cases
The pituitary gland is located at the base of the brain, just below the hypothalamus. Being just the size of pea, the pituitary gland has huge role in the brain. It has three sections know as the anterior, intermediate, and the posterior lobes. To begin, the anterior lobe is primarily involved with the development of the body, maturation, and reproduction. The hormones produced by the anterior lobe adjust growth, stimulates adrenal and thyroid glands along with the ovaries and testes. The anterior lobe also generates prolactin which allows new mothers to produce milk. Then there is the intermediate lobe which releases a hormone that stimulates the cells that control pigmentation known as the melanocytes. Lastly, there is the posterior lobe
There are different type of nuclear receptors, types (I-IV). Steroid hormone receptors such as the estrogen receptor is type I. Type I undergoes nuclear translocation from cytoplasm into the nucleus upon ligand activation (hormone-receptor complex). The Hormone diffuses through the cell membrane where it binds to the nuclear receptor/heat shock protein in the cytosol. Upon the hormone binding, the heat shock protein dissociates. Dimerization then takes place resulting in two homodimers where it trans-locates into the nucleus, binding to inverted repeat DNA half sites called Hormone Response Elements (HREs), found within a promotor of a gene. In the case of oestrogen, it binds to oestrogen response elements. The nuclear receptor/ DNA complex