To identify systematic error the experiment must be repeated. When repeating the experiment new apparatus or materials must be used, such as new stock solutions of hydrogen peroxide. If the new data differs consistently from the original data this indicates systematic error is present. Strengths Strengths throughout the practical are the simplicity of the overall experiment as it only took one double lesson to complete the whole practical and the materials needed to conduct it were easily accessible. The experiment was also safe, as it did not contain many substances or equipment that were dangerous or considered unsafe. Another strength could have been our ability to finish this practical in an appropriate manner, using the correct method and equipment. Another strength in the investigation was that all liver cubes were derived from the same source of liver. All liver cubes used for each of the seven experimental groups came from the same sheep’s liver. It is possible that different livers have different concentrations of catalase, therefore, different rates at which they breakdown hydrogen peroxide into …show more content…
For example, potatoes and capsicum are catalase rich substances and could easily be substituted in for the liver. They would still have the same effect as the liver on decomposing hydrogen peroxide and would produce valid results. Additionally, cutting potatoes or capsicum into precise 1cm x 1cm x 1cm cubes would be a lot easier and more accurate than cutting the liver into cubes, due to their ridged nature. Therefore, the size and shape of the cubes would be more accurate, thus, the surface area would be more consistent throughout all of the cubes reducing random error from this source. Lastly, using mechanical cutters rather than a knife as this could provide cubes of a more consistent
Triple Sugar Iron Agar test, there was a gas production seen in the media. The media was yellow slant and yellow butt indicating glucose, lactose and/or sucrose fermentation with acid accumulation in slant and butt. For sulfur reduction, it was negative since it did not turn black in color indicating no sulfur was reduced.
In this experiment, the naturally occurring peroxidase is extracted from homogenized turnip (Brassica rapa) pulp (Coleman 2016). Its role in the environment is to remove toxic hydrogen peroxide during metabolic processes where oxygen is used (Coleman 2016). The goal of this experiment is to evaluate the change of absorbency of turnip peroxidase within a metabolic reaction utilizing oxygen. Any change noted is indicative of the peroxidase removing hydrogen peroxide. Within this experiment, the extract will be prepared, the amount of enzyme will be standardized, and the effect of changing the optimal conditions will be observed. If the enzyme concentration is increased then the rate of the reaction decrease. If the pH of solutions used is increased
My unknown organism #6 is Morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora. (3, 5) This bacterium Morganella morganii, was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide distribution, it is an uncommon cause of community-acquired infection and is most often encountered inpostoperative and other nosocomial settings. (2, 3) Morganella morganii infections respond well to appropriate antibiotic therapy; however, its
The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar (MSA), Blood Agar, Novobiocin, Coagulase, and DNAse (Alachi, 2007).
Unknown lab report# 24 Introduction Microbiology is a study of organisms that surrounds us every day. It requires an educational understanding to identify organisms, and the ability to distinguish different bacteria’s. In applying the learning process of the different bacteria’s, unknown bacteria samples are given to be studied and identified.
The topic of this lab is on biochemistry.This experiment was conducted to show how cells prevent the build of hydrogen peroxide in tissues. My group consisted of Lekha, Ruth, and Jason. There were used two different concentrations of hydrogen peroxide through this experiment , 1.5% and 3%. By testing two different types it is easier to understand how the H2O2 and catalase react with one another. To do this both the yeast, which was our catalase, and H2O2 were mixed together in a beaker. Each concentration was tested out twice for more accurate results . 1.5% concentrated H2O2 had an average reaction rate of 10.5 seconds while 3% concentrated H2O2 had an average reaction rate of 7.5 seconds. From this experiment we learned that by increasing the concentration of H2O2 and chemically combining it with a catalase it will speed up the reaction. Enzymes speed up chemical reactions . The independent variable in this experiment was the concentration of the H2O2. Some key vocabulary words are Catalase, enzyme, hydrogen peroxide ( H2O2), and concentration.
An unknown was given to our group from the professor. The unknown was in nutrient broth, the group received unknown number 3. And the task was to identify the unknown and try to make an educated guess, and identify the unknown #3.
In this above reaction, oxygen is released and is used for other cellular purposes, but when it occurs in a test tube, similar to this experiment, the oxygen gas bubbles producing a layer of foam on the surface of the peroxide. The amount of foam and the speed it is produced are forms of measuring the catalase activity. In the next experiments, one would determine the degree of catalase action by calculating the thickness of the foam layer. It is hypothesized that when reacting with: potato, apple, steak, or liver, the plants and animal tissues will react differently.
We could use the H2O4 because acids will interfere with hydrogen bonds and other IMFs in the enzyme which would denature it, permanently making the enzyme unable to work. The catalase was the enzyme that acted on the product, which was hydrogen peroxide (H2O2). And of course, the hydrogen peroxide, or H2O2, was the substrate and the catalase was the enzyme.Our results seemed to be consistent because once we added the necessary amount that was needed to determine the amount of H2O2 in the solution, the color of the solution afterwards would always be a light, clear brown, as opposed to having various colors and shades each time. This means that we added the correct, proportional amount of potassium permanganate each time. In comparison to the class data, less of our H2O2 was decomposed. For example, for the trial for 30 seconds, 0.6 mL of our H2O2 was decomposed while the average for the class data was 2.22
It takes about 15 days for lab results to be complete once samples are received. The FADL emails lab results to the requestor and will CC you in the email. It is important that you keep track of the lab results; you will need to make a deposition for all lab positive or failed lab results. Notify CW5 Finch or APHC representative when samples contain pathogens. AR 40_657.pdf Chapter 5 section 5-6 give detailed instruction for nonconforming lab samples. When you receive a positive sample lab result that is non-pathogenic, you will make the decision on the which course of action to take. Recently I received lab results for product that had a high SPC counts. I contacted the facility management address the issue, issued the manager
Introduction: Throughout the coarse of the lab experiment the decomposition of hydrogen peroxide (H2O2) was done using catalase (an enzyme usually found in living organisms like yeast). The yeast acted as the substance that speeds up the decomposition of the reactants in H2O2 without changing its mass (not undergoing permanent change). Hydrogen peroxide is a complex compound that is commonly found in reaction that involve living organisms/cells, the function of the catalyst, otherwise known as yeast in this is experiment, with it catalase enzyme is to break down the compound H2O2 into smaller molecules of H2O (water) and O2 (oxygen). The enzyme catalase is built and shaped to allow the break down and decomposition of H2O2 to occur. Yeast
There is a large amount of catalase found in a human liver. Does the liver break down more hydrogen peroxide in the summer or winter? Explain your answer.
I read your comments on my lab report and understand why I missed the points that I did. I didn’t miss many points but the ones I did miss included simple mistakes like word choice. For instance, I wrote “by hand” at one point when I should have written “manually.” A similar mistake was when I noted that a wider variety of ages would lead to “…more conclusive results” when I shouldn’t have used this wording because our results were conclusive. My abstract had the most mistakes. I was more specific than I should have been. I included too many details and I now understand why they were unnecessary. Other than those few mistakes, my report was pretty solid. This is reassuring because I put a lot of time into it. I worked diligently to ensure
The aim of my investigation is to see how pH affects the activity of potato tissue catalase, during the decomposition of hydrogen peroxide to produce water and oxygen.
The differences for the rates of reaction between the liver and potato are accounted for because liver contains more catalase enzymes than potatoes. This is because the liver is responsible for ridding toxins out of the body and as a result needs more catalase enzymes to do so; explaining the bigger reaction it had with the hydrogen peroxide.