T Even Bacteriophage Research Paper

6. Humans should not be concerned about the bacteriophages infecting other cells because each bacteriophage is particular to a certain bacteria. If the bacterial cell exhibits traits that are desirable to the certain bacteriophage, then the phage will chose to bind and infect it, otherwise people have nothing to worry about.

When we hear the word virus, we usually think of something that makes us sick. A virus is an ultramicroscopic infectious organism that, having no independent metabolic activity, can replicate only within a cell of another host organism. A virus consists of a core of nucleic acid, either RNA or DNA, surrounded by a coating of antigenic protein and sometimes a lipid layer surrounds it as well. The viral genome provides the genetic code for replication, and the host cell provides the necessary energy and raw materials. To fight viruses, we use vaccines. While some may infect a broad range, other viruses can only infect certain kinds of cells. Vaccines are made of inactive, dead or weakened virus cells or protein antigens that can no longer infect

Viruses are microscopic particles that invade and take over both eukaryotic and prokaryotic cells. They consist of two structures, which are the nucleic acid and capsid. The nucleic acid contains all genetic material in the form of DNA or RNA, and is enclosed in the capsid, which is the protein coating that helps the virus attach to and penetrate the host cell. In some cases, certain viruses have a membrane surrounding the capsid, called an envelope. This structure allows viruses to become more stealthy and protected. There are two cycles in which a virus can go into: lytic and lysogenic. The lytic cycle consists of the virus attaching to a cell, injecting its DNA, and creating more viruses, which proceed to destroy the host. On the other hand, the lysogenic cycle includes the virus attaching to the cell, injecting its DNA, which combines with the cell’s DNA in order for it to become provirus. Then, the provirus DNA may eventually switch to the lytic cycle and destroy the host.

The overall goal is to either discover a new novel phage that has not yet been discovered, or provide new research on an already discovered phage. The isolation of the phage takes several steps under the streak protocol that is present in materials and methods section of this report.

The titer of recombinant phage was determined with the number of plaques on the E.coli K plates and the titer of total phage progeny was determined with the number of plaque on the E.coli B plates.

Titer of the Bacterial virus, the Measurement of the Recombination and the Reversion Rate, and the Gene Map Distance (Phage Recombination)

The virus fuses with the cell’s plasma membrane. The capsid proteins are removed, releasing the viral proteins and RNA. Reverse transcriptase catalyzes the synthesis of a DNA strand complementary to the viral RNA. Reverse transcriptase catalyzes the synthesis of a second DNA strand complementary to the first. The double-stranded DNA is incorporated as a provirus into the cell’s DNA. Proviral genes are transcribed into RNA molecules, which serve as genomes for the next viral generation and as mRNAs for translation into viral proteins. The viral proteins include capsid proteins and reverse transcriptase (made in the cytosol) and envelope glycoproteins (made in the ER). Vesicles transport the glycoproteins from the ER to the cell’s plasma membrane. Capsids are assembled around viral genomes and reverse transcriptase molecules. New viruses bud off from the host cell.

The main objectives of this experiment included making dilutions of solutions, plating phage or bacteria, and determining the number of bacterial viruses or phage in a suspension. It was also conducted to demonstrate that two different mutants of phage T4 can exchange genetic material to give rise to wild-type phage. The experiment was used to distinguish mutants from wild-type by their host specificity. The recombination in bacteriophage was performed to determine the concentration of unadsorbed phage from the U series plates, total concentration from B series, and concentration of

This project is all about isolating bacteriophage in soil. They come in different sizes and shapes, each to their own unique look. Phages have a protective protein head that contains DNA and a hollow tube tails (http://phages.org/bacteriophage/). Since bacteriophage cannot reproduce and replicate themselves, they need a host to do the work for

For example, viruses do not use energy and raw materials to grow and reproduce, and they are not made of one or more of the basic fundamental units of life – a cell. Compared to a cell, which includes organelles that enable the structure to grow and reproduce, a virus can only reproduce if another cell is present and that cell has organelles that the virus can inject its own DNA or RNA into and use it for its own replicating or mutating purposes. Viruses are made of a protein coat or capsid with its DNA or RNA genetic material enclosed inside of the capsid. In comparison, in addition to having organelles that use energy to help the cell develop, grow, and reproduce with the help of DNA, the cell by definition is enclosed in a membranous boundary,

A virus is a capsule containing genetic material, even smaller than bacteria. The main task of virus is to reproduce. However, viruses need a suitable host to

Using FinchTV, it was determined that each sequence had only one Sal I digest site, suggesting that each clone was not the full length of the inserted fragment. However, this was not a problem because of the two primers that were inserted as a pre-emptive solution for this instance. All four clone fragments also matched to the phage Urkel, with 98-99% certainty (NCBI and PhagesDB). Using PhagesDB, Urkel was found to be K1 cluster phage. When a BlastX search was performed using NCBI, the first clone fragment did not match to any known functions. However, the second clone fragment matched to having a minor tail protein from the T3 fragment and a tape measure protein from the T7 fragment, both of which were to the phage SamuelLPlaqson (NCBI). This suggested that this fragment had remnants of both genes. In an effort to explain these results, the previously annotated phage genome of Phrank was analyzed. Phrank’s genome revealed that the tape measure protein often precedes the minor tail protein by merely a few base pairs. Using this information, this fragment was confirmed to have partial genes of both the minor tail protein and the tape measure protein; the NCBI BlastX results were different for the two primers because the search was showing the most likely match given the length of the respective fragments. Due to Zassy’s high

Each batch of phage was used to infect a different culture of bacteria. After infection had taken place, each culture was whirled in a blender, removing any remaining phage and phage parts from the outside of the bacterial cells. Finally, the cultures were centrifuged, or spun at high speeds, to separate the bacteria from the phage debris.

Lab number 6 was a success. The Phages were isolated nicely. The only negative in the results was that they were not easily seen or transparent in the light. The plaques were isolated, large, and opaque. One plate had an isolated Phage with some small and white contaminates near the Phage. The second Agar plate did not have contaminates, but as stated before, the plaques weren’t transparent circles. The large plaques were faded.

The protective capsid helps the virus escape detection and destruction during the invasion of the host. When the virus reaches the target cell, biochemical reactions between the capsid and cell wall allow the virus to latch on and inject its genome into the cell’s interior. Once inside, the viral genetic material insinuates itself into the host’s DNA or RNA. In an efficient feat of natural bioengineering, the host cell’s genetic machinery now does the rest of the work for the virus. The cell, which had already been making copies of its own genome, now also replicates that of the virus. Coded within the viral material is the blueprint for making more copies of the viral genome. Further instructions command the production of capsids and directions for assembly of new viruses. After the host cell becomes engorged with viruses, it explodes, sending the new