Tb Is A Complex, Chronic Disease

1787 WordsAug 27, 20168 Pages
TB is a complex, chronic disease which induces immune dysregulation of several arms of the immune system. In this study, we analyzed the specific concentration of IFN-γ, TNF-α , IL-1β, and IL-10 cytokines from smear negative PTB(SNPTB) and smear positive PTB (SPPTB) to determine the effect of mycobacterial load on the cytokine concentration utilizing a simple whole blood assay stimulation with a whole sonicate MTB. Different concentration of cytokines(IFN-γ, TNF-α, and IL-10 ) detected in present study were associated with bacillary load of MTB in sputum from PTB patients. Our results demonstrate that , individuals with smear-negative PTB was associated with increased concentration in Th1 cytokine IFN-γ and decreased TNF-α ,IL-1β , as well as decreased concentration of specific Th2 cytokine IL-10 as compared with individuals with smear positive. These results strongly suggest that the sputum positive group had the lowest immune response (lowest IFN-γ levels, and highest IL-10 level) and indicate that the cytokine concentration is progressively impaired with increased mycobacterial load. In addition, PTB patients presented with a higher expression of IL-10 and TNF-α in contrast to the lower expression of IFN-γ and IL-1β as compared with healthy control. IFN-γ secreted in low concentration in two clinical PTB patient groups(SNPTB,SPPTB) as compared with health control(HC), but these levels insignificant between two
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