Introduction
The purpose of this experiment was to test whether the soil from outside the Suprenant building contained the highest level of bacteria out of our three samples: soil, human lips, and a toilet seat. We determined that the soil should contain the highest level of bacteria. A teaspoon of soil can contain anywhere from 100 million to 1 billion bacteria (Ingham n.d.). This was the basis for our assumption that the soil would contain the highest levels of bacteria. If the soil contains the highest levels of bacteria, then the agar plate with the dirt sample will grow the most bacterial colonies.
Bacteria is the foundation for life on earth as we know it. About 4.5 billion years ago the even that sparked life on earth happened, and that life was bacteria (Gould 2012). The bacteria of that time used anaerobic respiration, which is much less efficient than aerobic
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We had the Biology Department staff put the 3 petri dishes in the incubator at 37 degrees Celsius for a 24 hour period. After that they were kept refrigerated an additional 6 days. One plain agar plate should have been incubated as well to provide a control for our experiment, but it was not.
Results
Human lips, the soil outside of QCC, and a toilet seat were all tested for bacteria content. All three of the things tested: human lips, soil, and toilet seat had some bacteria content that grew. The human lips had the most amount of bacteria at 15% (Table 1 & Figure 1). The toilet seat had 8% of bacteria (Table 1 & Figure 3). The soil outside QCC had only 5% bacteria (Table 1 & Figure 2). The toilet seat had two different kinds of bacteria on it (Table 1 & Figure 3). Fungus was only present in the sample collected from the soil outside QCC (Table 2 & Figure 3). Figure 1: Human Lips Figure 2 : Soil Outside QCC Figure 3: Toilet
The result of this experient shows that lunch meat grows more bacteria than Skittle and bacteria does grow more if we drop it. In the result of lunch meat, tile floor grows the most bacteria and for the Skittle, bacteria grows more on the cotton cloth. During the experient, I find out that it’s interesting that after 12 hours, that control sample for lunch meat grows more bacteria than the lunch meat on cotton cloth.
Bacteria can exist almost everywhere, some are harmless, and some are harmful. There are thousands of different types of bacteria and they fall
When reflecting back to experiment 3, Aseptic Technique and Culturing Microbes, I realized the large amount of microorganisms that can be found in everyday life. Many different types are found with in the human body. Theses experiments focused on two types of bacteria. First was Staphylococcus epidermidis, found on the skin, and second was Lactobacillus acidophilus, found in the gastrointestinal tract. Both have similar needs for growth when it comes to temperature, however, different growth environments are used.
After collecting all eight samples, they were then placed in the Petri dishes. The Petri dishes were both placed in an incubator. The temperature at that time was 80 degrees Fahrenheit. The samples remained in the incubator for over 24 hrs, by which time, quite a lot of growth was formed.
There were two types of bacteria: sulfur-eating bacteria, and cyanobacteria. Sulfur eaters were the predominate kind of bacteria, but died out when cyanobacteria started photosynthesizing and producing oxygen. Oxygen is lethal to sulfur-eating bacteria. This was called the great oxygenation. The sugars produced by photosynthesis would be the base of the modern food chain. The origin of archaea is unknown. Eukaryotes came into play around 1.5 billion years ago.
The purpose of the following study is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature, pH level, and osmoregularity. It is important to classify bacteria in order to identify them. Identification of bacteria is important because they are not only useful but potentially dangerous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. Identifying bacteria can also be used in many other areas from better crop production through microbial pesticides to biological warfare. Their uses are endless as are their abilities to evolve and adapt to changing environments. That is why it is so important
Microbes are bacteria, archaea and eukaryotes. The earth was formed 4.6 million years ago. And a few million years later, by 3.5 billion years ago, earth was already inhabited by a diversity of organisms. The earliest organism is Prokaryotes and within the next billion years, two distinct groups of prokaryotes called bacteria and archaea diverged. Eukaryotes cell evolved from a prokaryotes community, a host cell containing even smaller prokaryotes .The microbial world accounted for all known life forms for nearly 50 to 90% of Earth's history. We are still researching microbial organisms today in marine environment, extreme environments. A microbial observatory is an NSF-funded project dedicated to the discovery and characterization of novel microorganisms and microbial communities of diverse
After gaining some knowledge about bacteria, we were giving an investigating bacteria growth lab to do. Our objective was to observe the conditions required for bacteria to grow and to test the effectiveness of substances that may be antibacterial, disinfecting, and or sanitizing. My group and I began our procedure by gathering all the bacteria by swabbing our necks and mouths. After this, we inoculated the culture by rubbing the bacteria on the agar, a nutrient rich gel made from sea kelp, on the bottom side of the container where we grow bacteria, the Petridish. We hoped for the results to come back with little or even no colonies and an immense zone of inhibition around the tiny circle cut out of filter paper covered in toothpaste, Neosporin, and Chlorhexidine Gluconate 4% Solution.
Accurate evaluation of bacterial colonization as a predictive index for alfalfa sprouts has relied on a quantitative culture technique that provides exact colony counts per gram of tissue by culture of five serial dilutions of the alfalfa water. In this study 1 package of alfalfa sprouts were cultured by a semi-quantitative technique that enumerated the number of gram-negative enteric organism in 1 ml of alfalfa water. Exact colony counts from the experiment were available only from cultures 10-2 and 10-3 CFUs. Other colony counts were reported as less than 10-3 or greater than
Epidemiology – the science that studies when and where diseases occur and how they are transmitted. CDC, Atlanta, tracks and traces diseases. ONE World (the idea that it’s all in one place, it could happen anywhere; locally, states, large counties track the incidences and occurrences of disease)
: In the field of microbiology, there are times when a sample will contain more than one species of bacteria. The goal is to separate each bacterium and culture them independently from one another to identify them. This was the objective of this lab. Each stock contained two unknown bacterium, and the possible unknowns were Eserichia coli, Enterobacter aerogenes, Enterobacter cloacae, Serratia marcescens, Proteus vulgaris, Klebsiella pneumoniae, Shigella flexneri, Shigella sonnei, and Salmonella enterica. The tests available were MacConkey agar, Endo agar, Hektoen Enteric agar, Tryptone Soya Agar (TSA), carbohydrate sugar broths, Triple Sugar Iron (TSI) agar, decarboxylase broths (arginine, lysine, and ornithine), Simmon’s Citrate Agar, urease
Primitive bacteria originated during the Precambrian time. Scientists believe that during the Precambrian time primitive bacteria was first found, and is found, in the bottom of the sea floor in hot springs about four billion years ago.(Encarta) It is believed that four billion years ago bacteria first consumed food by ingesting organ molecules or chemosynthesis which is the reaction involving chemicals. The first bacteria were one celled cyanobacteria and is trapped in the sediments of the Earth’s crust. Bacteria started from one celled organisms and lead to the creation of two-celled organisms.
In the beginning of lab, we were advised to obtain a nutrient agar petri plate, which is used for the cultivation of microbes supporting growth of non-fastidious organisms. Since it contains many nutrients, a wide variety of bacteria and fungi can grow. Taking the plate,
In everyday life, humans endure micro-organisms, bacteria, and fungi, due to Microbial contamination. This phenomenon occurs frequently, and people are unaware of the causes and effect of what microbial contamination can have to truly offer. By definition microbiological contamination occurs when non-intended or accidental introduction bacteria, yeast, mold, fungi, virus, prions, protozoa or their toxins and by-products is carried from one object to another object, person to person, or from an object to a person. (Sue,2007) Cross-contamination occurs during cooking. Specifically, it occurs when juices from raw meats or germs from unclean objects touch cooked or ready-to-eat foods.(Eleese Cunningham, 2015) or even in equipment at processing facilities not being cleaned properly, for instance, or any of the other numerous and sundry ways food can be mishandled a make it to someone, which can greatly increase the risk of food poisoning. (Danilo Alfaro, 2017) Cross contamination does not only consist of bacteria but furthermore, it consists of a virus or a toxin of some sort. (Danilo Alfaro, 2017). It ranges from complex multicellular eukaryotes to even the most unicellular prokaryotic organism, and at any crack or even any type of opening at West Carleton, there will be definitely living microorganisms being passed along from one person to another unconsciously.
Each mixed culture that was tested had one gram positive and one gram negative bacterial species. The possible species of bacteria that could have been isolated from the mixtures included the following: Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella enterica, and Pseudomonas aeruginosa. The identities of the unknown species were determined through comparing the experimental data against data acquired from earlier experimentation.