Temperature And Enzyme Activity

Introduction: Starting out with some background information, I know that enzymes are biological catalysts. The enzyme that I used for this experiment was potato juice. Enzymes make reaction rates go faster. They lower activation energy, making chemical reactions. Temperature has an effect on canola cultivars. The higher temperature decreased stem diameter, but room temperature had thicker stems. So I believe the same will happen for the catechol oxidase; the solution will react faster at room temperature. Other enzymes can also have different effects such as the enzyme in cattle serum. The enzyme lost activity in room temperature. With that being said room temperature can also be detrimental with specific enzymes. Fungus also

The Enzyme Properties Lab was performed to analyze the effects of pH, enzyme concentration, and temperature on the catalytic rate of enzymes. Many functions within the human body require/involve enzymes. It was found that enzymes have an optimal pH level, catalytic rate increases as the concentration of the enzyme increases, and an optimal temperature. The enzyme that was used throughout the experiment was ALP (alkaline phosphatase enzyme). ALP functions best at a neutral pH level, as it had the largest calculated enzyme activity of 2.05E-03. When pH went above or below the neutral value the enzyme activity tended to fall. ALP’s calculated enzyme activity tended to increase with an increase in enzyme concentration, as the calculated enzyme activity of the high enzyme concentration environment was 0.004607, which was larger than the other conditions. The last part of the experiment involved temperature and it was found that the optimal temperature for the enzyme was 32°C with a calculated enzyme activity of 0.000353. When temperature was below or above the optimal temperature the calculated enzyme activity dropped. Enzymes can begin to change shape and lose function when conditions are not appropriate, which was seen in the enzyme activity of ALP in varying environments. It can be concluded that enzymes play a major role in many reactions, but if conditions (pH, enzyme concentration, and temperature) are not appropriate enzyme activity tends to drop. The results supported the

    The objective of this experiment is to explore the effect of temperature on the enzyme peroxidase. To comprehend the effect that temperature can have on enzymes, specifically peroxidase, one must understand what enzymes are and what their function is. Enzymes are proteins that are found in cells that function as catalyst (Ms. Chang's Enzyme Notes). What is meant by this is that enzymes increase the speed of chemical reactions without changing the chemical equilibrium between reactants and products or becoming consumed or permanently modified by the reaction through lowering the activation energy barrier (Enzymes). Enzymes are necessary in order to sustain life because without them, the chemical reactions that take place in our cells would occur far too slowly (Ms. Chang's Enzyme Notes).

The effects of temperature, pH, specificity and cofactors of enzyme activity were tested. An enzyme is an organic catalyst that speeds up metabolic reactions by lowering the activation energy in cells. Activation energy is the minimum amount of energy needed for the chemical reaction to occur and can be affected by altering the enzyme’s environment as shown during the experiments. When the enzyme is not at the optimal temperature or pH, then the enzyme may become denatured, inactive and lose its normal shape. The enzyme used was Lactase, which binds to the substrate lactose. A substrate is the reactant in the enzymatic reaction and binds to the contact region on the enzyme called the active site. Enzymes have three dimensional shapes

This experiment was performed in order to determine how the condition of the environment and the specificity can affect enzymatic activity. The activity of enzyme, how efficiently the enzyme functions, is affected by general environmental factors, such as temperature and PH. It can also affected by chemicals that specifically influence that enzyme. In condition of low temperature environment, the enzymes activity will be low. As the temperature raises the rate of an enzyme-catalyzed reaction increases. The optimal temperature of an enzyme is where the temperature that the enzyme works best. Enzyme specificity is the ability of enzyme to choose exact substrate from similar molecules. Lactase is an enzyme found in small intestine that catalyzes the breakdown of Lactose (milk sugar) into the simple sugars glucose and galactose. In this experiment the enzyme Lactase and its’ substrate Lactose were used to measure the production of Glucose. This experiment will examine the specificity of an enzyme to only a specific substrate and the amount of glucose production in different temperature.

John Cole PBIO 1210L Scientific Paper 29 October 2014 Temperature effects on Enzyme Activity Cole-Barker PBIO 1210L W 1:00-2:55 Introduction: The main purpose of enzymes is to catalyze or speed up reactions that happen in all plant and animal cells. The majority of all enzymes are proteins. These proteins work in plant and animal cells to speed up or catalyze reactions. They do this by lowering the activation energy which is the minimum amount of energy required for a reaction to take place.

From the results I can conclude that enzymes are heavily affected by temperature changes; slowing down the rate of a reaction if the conditions are wrong. The amount of oxygen that was produced decreased as the temperature

Enzymes only work within certain temperature and pH ranges as they can be denatured at certain temperatures and pHs. When the enzyme is denatured, the shape of the active site on the enzyme will change. Once

Enzymes are proteins that work to expedite processes and enhance reactions that occur within cells. Furthermore, enzymes act as catalysts, in which they cause a reaction to be accelerated. However, if these enzymes are exposed to an abnormal environment or withstand extreme conditions such as an increase or decrease in temperature or pH, they will become inactive. Proteins, such as enzymes, must be in a three dimensional structure with active sites to function properly. Acidity, temperature, and concentration all affect the structure and molecular base of amino acids (Controlling Enzymes, 2015). If temperature is decreased, enzyme activity will decrease as well. If temperature is increased, enzyme activity will rise. However, if the temperature

: Enzymes are made up of proteins and their function is mainly determined by their structure. The primary structure of an enzyme is determined by the sequence of amino acids. The secondary structure is based on the folding of the primary structure. Lastly, the tertiary structure is based on the folding of the secondary structure and its interactions of the side chains. The purpose of an enzyme is to speed up chemical reactions in a cell. Temperature is a factor that affects enzyme activity because as temperature increases the enzyme reaction increases too. Also, pH levels also play a role in affecting enzyme activity. Very high or very low pH levels can result in the loss of enzyme activity. For an enzyme to maintain activity it needs to be

Enzymes are biological catalysts that carry thousands of chemical reactions that occur in living cells. They could be found in every living thing. Enzymes increase the rate at which chemical reactions occur. A catalyst speeds up a chemical reaction by lowering the activation energy required. They are proteins made up of several amino acids. Each enzyme is different due to its structure, it all has different amino acid sequence. An enzymes ability to function depends on it’s environment. These factors include temperature, denaturation, pH, the size , the shape, and the amount of enzymes present. Enzymes performs best at ph7. That is the same pH as blood , the more enzymes stray away from pH7 the weaker it becomes , the active site gets damaged. This makes the enzyme not active, no longer functional. When it comes to temperature enzymes at a certain temperature have maximum activity. As temperature increases the enzyme activity also increases. When enzymes reach their maximum activity level and the temperature is still increasing it will start to decline (denaturation). The rate of reaction also depends on how much enzymes are present. We tested 5 hypothesis which were the amount of enzyme does not alter the rate of a reaction. pH does not influence the rate of an enzyme reaction. Temperature does not influence the rate of an enzyme activity. Boiling an enzyme before a reaction does not influence enzyme activity. The

We are investigating the relationship between enzyme’s Digesting speeds in different temperatures. We are going to measure the speed of amylase (Dependent) in different temperatures (Independent).

Enzymes offer support to the human body. They speed up cells’ chemical reactions within the body by lowering the activation barrier. The goal of this experiment was to understand how and why enzymes work in such specific ways. It was also to understand the different conditions under which the enzymes work.

According to the graph, it shows that the enzyme-catalyzed reaction performs the best as the temperature increases to 30’C. The rate declines as the temperature increases over 30 and it no longer works at 70. When the surrounding temperature increases to 30, the reactant absorbs enough energy to top of the activation energy barrier and the reaction occurs. As the temperature inclines, there begins an increase in molecular movement which leads to an increasing rate of reaction, in this case, producing more bubbles of gas. However, as the temperature get high, the transition state of molecules is reached rarely that the reaction will hardly proceed at all. Therefore, in the environment where the temperature higher than 30C, the molecular movement

The efficiency of enzymes depends on a number of factors, such as temperature, pH and the concentration of the enzyme just to name a few. This experiment was conducted to show the effects of different pH levels on the activity of the catalase enzyme, the data obtained in this experiment supports the initial hypothesis of the experiment which states that the catalase enzyme will function optimally at a pH of 7 and efficiency will decrease as the pH of solutions moves further away from 7. This is true because in the experiment the test tube containing the distilled water which has a pH of 7, produced the highest amount of foam out of the four test tubes. The efficiency of the reaction can be determined by the amount of foam (foam