Essay Test for Starch and Reducing Sugar Present in Apple and Pear

At five minute intervals over the next fifteen minute period, record the color intensity of the solution of each test tube.

Test tube six contained an elodea and was placed in the dark. It was hypothesized that, after a day, the test tube would be yellow in color. Upon observing, the hypothesis was proven correct because test tube six had remained yellow. This is because the elodea was placed in the dark where it could not receive light to photosynthesize and remove carbon dioxide from the

H. How would you prepare 10 mL of a 0.25M HCl solution if 1M HCl was available? How much

The null hypothesis will be that the test tubes with an increase in temperature, pH values, enzyme concentrations, and substrate concentration will have a very small color change or no color change at all. The alternate hypothesis is that the test tubes containing an increase in temperature, pH values, enzyme concentrations, and substrate concentration will all have an intense color change; the more the change, the more intense the color change will be.

After carefully taking them out, observe each test tubes color and then fill Table 2 with the results.

7 Corvettes These are used instead of test tubes as corvettes are required if a colorimeter is to be used. One corvette is needed for each pH and one for the control. These can then be reused for the repeats. Syringe calibrated at 0.1 cm³ intervals

deionized water to make a 250 mL solution, the molarity of that solution would be 0.00935 M.

2. Which of the lab values gives you information about how Mrs. Burroughs’ kidneys are functioning?

4. We observed the test tubes and compared the colors produced from the reaction to the color palette in 1 minute intervals for 5 minutes total.

Though the theory following the hypothesis is correct and the experiment was carried out with as much attention as possible in a high school laboratory, the results obtained were still indicative of a few errors and did not support the predicted hypothesis. From the results obtained it can be concluded that as the concentration of sucrose increases the average percentage change in mass decreases. This is because the salt concentration inside the potato cubes of 10%, 15% and 20% concentration is less that in the salt concentration on the sucrose solution, thus the three cubes submerged in the 10% - 20% concentrated solution lost mass (hypertonic). However the cube submerged

To perform this test, a tube of broth rich with glucose is acquired. In this tube is phenol red, a pH indicator. Initially, the tube appeared pink in color, indicating a normal pH level. Next, a sample of unknown #44 is introduced into this medium using the aseptic technique, and this is allowed to sit for several days. If the organism is able to ferment glucose, the pH in the medium would decrease and cause the phenol red to exhibit a yellow color. In addition to the straw color, gas can also be produced and trapped inside the Durham tube placed in the medium. This production of acid and gas is a direct result of the fermentation of glucose, as seen with unknown

apples and some of which were shaped like eggs, some were small and others were large."

Table 2: Consists of color extract taken from a red cabbage for a natural indicator. The pH reading that was measured by using the pH meter and the result of the pH reading to determine whether the solution was acidic or basic.

3. Use a sterile pipette to transfer 0.1 ml of each dilution on to a MacConkey agar plate.

After the 30 minutes, the color was observed and recorded on the data sheet. The dialysis tubing was removed from the beaker and a small slice was made, we then used a glucose indicator strip to test for the presence of glucose, along with the solution in the beaker. The results were then recorded in table 1on the data sheet.