Testing On The Organism You Provided From One Of Your Patients

Proper collection of blood cultures are necessary and the most direct method of determining whether or not a patient is septic. The purpose of obtaining blood cultures is to identify and isolate the bacteria that are causing an illness and then determine the best course of treatment based on the sensitivity of the bacteria to particular antibiotics. One of the most frustrating problems plaguing hospitals is the increased rate at which blood culture results are being returned as contaminated specimens. These results can lead to a significant increase in cost to the hospital and patient as well as an increased length in hospital

Essentially the growth and metabolism of any micro-organisms are profoundly affected by the temperature. For example, for Saccharomyces it’s said to increase the production of alcohol increases with temperature up to 40° (Brown 1914). Any organisms (in order to survive) require adenosine triphosphate (ATP), ATP is produced using cellular respiration. Cellular respiration are metabolic reactions that take place in order to produce ATP, which is essential for any cellular activities whether it is movement, work or temperature maintenance. Cellular respiration involves glycolysis followed by citric acid cycle including the Krebs cycle and oxidative phosphorylation. The most amount of ATP is produced in the Krebs cycle

Spread occurs via oral, vaginal, and anal sexual contact through the routes of penis-vagina, penis-mouth, penis-anus, mouth-vagina, and mouth-anus. During childbirth, infants contract the infection in the birth canal resulting in bilateral conjunctivitis. Intimate contact is required to infect another person, therefore, the myth of contracting the disease by toilet seats is a myth.

Gold standard procedures should be implemented with the aim of providing timely and accurate results. (Schentag) The culture result should be accompanied by its clinical significance, selective reporting of susceptibility testing results in accordance with hospital antimicrobial therapy guidelines and suggested management. (Schentag) This encourages appropriate prescribing and minimises unnecessary use of antimicrobials. The clinical microbiology team should also provide reports to AMS regarding resistant organisms.

The conditions needed for the growth of micro-organisms are: Micro - organisms need food to survive. They like high protein food to survive, eg. Poultry & fish. Most micro - organisms need warmth & grow best at 20-40c. They need moisture to multiply. They need air to multiply, though some can without. A single Micro-organism becomes two every twenty minutes.

during sexual contact. These bacteria can infect the genital tract, the mouth, and the rectum. It

This experiment was conducted to find the genus and species of an unknown bacteria prescribed by the lab teacher, which was unknown bacteria GA3 in my case. Identification of unknown bacteria techniques are used on an every day basis to figure out what type of bacteria it is and to find the best method of how to treat a patient with this bacteria (1). All five “I’s” of Microbiology were used in the testing for the unknown culture. Inoculation was used several times to put the unknown culture into agar plates or into biochemical test tubes. After Inoculation of these tubes or plates, they always were placed into the incubator for further growth and development. Isolation was used to make sure we got the correct bacteria we were testing for. After each further isolation, we gram stained the culture and inspected the culture under a microscope to further help in the identification process of the unknown bacteria. Multiple tests were done on the unknown culture to make sure we were confident in what kind of bacteria the unknown was.

This particular bacterium is not one that inhabits a specific geographical region as it colonizes within the human body. The disease is

The objective of the investigation was to perform multiple experiments that would lead to the identification of an unknown bacteria. This bacteria was causing a urinary tract infection on a patient. A sample of the bacteria causing the infection was received and analyzed. The first step was to isolate the bacteria to insure that sample was free of contamination. A MAC plate, a PEA plate, along with a Nutrient agar plate where used to produce pure colonies by using the quadrant streak method on each plate. After a 24 hour incubation period the colonies where isolated. The bacteria collected was inoculated into slants, nutrient broth for cultivation. A sample from the MAC plate and the PEA plate were collected and gram

The spread of infection requires three elements. The first is; A source of infecting microorganisms: This could be an exogenous infection which arises from microorganisms external to the individual and do not exist as normal flora. They usually have a preferred portal of entry like the gastrointestinal for Salmonella. Another source is endogenous infections which can occur when part of the client’s flora becomes altered and an overgrowth results e.g. Yeasts infection. Second requirement is means of transmission for the microorganism, which also explains the three primary modes of pathogen transmission. For example, vertical transmission is when a pathogen is

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.

After microorganisms enter the patient’s body they can go to the lungs and cause pneumonia. Central line-bloodstream infections occur when microorganism enter the bloodstream by the intravenous line. Microorganisms are transfer into the blood stream by inadequate hand hygiene or improper intravenous fluid, tubing, and site care practice. Multidrug-resistant organisms can cause infections of the blood, skin, or organ systems. These infections can arise due to the overuse or misuse of antibiotics, which can result in the microorganisms becoming more resistant to antibiotic therapy. Methicillin-resistant Staphyloccocus aureus and Clostridium difficile are examples of this kind of infection. Health care employees need to know the different types of hospital acquired infections and how they are spread to be able to effectively prevent them.

A great deal of cultural tests and physiological procedures were performed on the unknown before the conclusion of P. aeruginosa was made. The first test preformed was Gram’s staining which involves the use of crystal violet, Gram’s Iodine, Ethyl Alcohol, and Safranin. Gram staining is known as a differentiating stain, since it can determine the presence or absence of peptidoglycan in the cell wall. The unknown bacteria were Gram stain negative. After that the motility of the cell was tested using a hanging drop slide. The slide was examined, but the results were inconclusive due to the inability to make a clear distinction between motility and Brownian motion. This test was not

Like most microscopic organisms, gonococcus develops well in warm, clammy situations. It flourishes in the mucous film tissues of the urethra, genitals, throat, and rear-end. It is transmitted through any sort of unprotected penile-vaginal, oral-genital, oral-butt-centric, and genital-butt-centric contact with a contaminated person. Hence, any sexually dynamic individual is in danger of contracting gonorrhea. The microorganisms can be spread even without entrance or discharge. Newborn

The parasite is passed from an infected individual to a uninfected individual during sex. In ladies, the most ordinarily tainted piece of the body is the lower genital tract (vulva, vagina, or urethra), and in men, the most common contaminated body part is within the penis (urethra). During sex, the parasite is normally transmitted from a penis to a vagina, or from a vagina to a penis, however it can likewise