The Accessory Photosynthetic Pigment Of Cyanobacteria

at that temperature, there was the most amount of catalase activity. On the pH graph, the

The role of an enzyme is to catalyse reactions within a cell. The enzyme present in a potato (Solanum Tuberosum) is catechol oxidase. In this experiment, the enzyme activity was tested under different temperature and pH conditions. The objective of this experiment was to determine the ideal conditions under which catechol oxidase catalyses reactions. In order to do this, catechol was catalyzed by catechol oxidase into benzoquinone at diverse temperatures and pH values. The enzyme was exposed to its new environment for 5 minutes before the absorbance of the catechol oxidase was measured at 420 nm using a spectrophotometer. The use of a spectrophotometer was crucial for the collection of data in this experiment. When exposed to hot and cold temperatures, some enzymes were found to denature causing the activity to decrease. Similarly, when the pH was too high or low, then the catechol oxidase enzyme experienced a significant decrease in activity. It can be concluded after completing this experiment that the optimal pH for catechol oxidase is 7 and that the prime temperature is 20º C. Due to the fact that the catechol oxidase was only tested under several different temperatures and pH values, it is always possible to get a more precise result by decreasing the increments between the test values. However, our experiment was able to produce accurate results as to the

The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.

The cell membrane (Plasma membrane) functions to provide cell support, cell stability and control entry and exit of materials from the cell. This study was conducted to test the effects of environmental conditions such as the on beet root cell membrane (Beta vulgaris). Five trials using varied pH concentrations were tested and absorbance rates were monitored. The experimental results showed that the protein function decreased sequentially when the pH decreased. This allowed the betacyanin dye to leak out which created the color that was needed to determine the intensity and therefore the effect of the circumstances. This supported the hypothesis that the more acidic or basic the environmental condition around the beet cell, the more permeable the, membrane indicated by color intensity. Pigment leakage in the solution was analyzed by using a spectrophotometer.

The purpose of this experiment was to alter the cell membrane of the beets, in a given fashion, so that we can test how much betacyanin was able to cross the cell membrane of the beet through various treatment. In which after we would test the absorbance level of each treatment, run it through excel and observe which treatment was most effective at getting betacyanin through the cell membrane more.

Photosynthesis is a vital process that requires to utilize energy for plants. This experiment was done to evaluate the effects of carbon availability on photosynthetic activity. The aquatic plant Elodea densa was placed into sodium bicarbonate solutions of five concentrations ranging from 0.1% to 1.0%, in five independent trials (excluding the negative control treatment of water). The temperature and light intensity was constant. The results indicated a directly proportional relationship between the availability of carbon dioxide and the rate of photosynthesis of Elodea Densa, as photosynthesis continued to increase with increasing amounts of bicarbonate. The most O2 amount of oxygen produced was with the 0.7% NaHCO3 concentration and least with the control of water. The null hypothesis that stated carbon concentration does not affect rate of photosynthesis of the aquatic plant was rejected. The predicted hypothesis that an increase in bicarbonate concentrations results in an increase in the photosynthetic rate was accepted. In conclusion, there is a significant increase in photosynthetic activity as the concentration of NaHCO3 increases.

Abstract: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene, the most abundant carotene in plants, is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found further from the solvent font because it is less soluble in the solvent and has been slowed down by hydrogen bonding to the cellulose. Chlorophylls contain oxygen and nitrogen and are bound more tightly to the paper than the other pigments.

The medium contains sodium citrate that serves as sole carbon source and ammonium phosphate as the sole nitrogen source. These organisms produce the enzyme citrate-permease that can transport the citrate into the cell and produce pyruvate from it. Bromthymol blue dye is used as an indicator that is green at pH 6.9 and blue at pH 7.6 and above (Leboffe and Pierce). A positive result is obtained if the color is blue meaning that bacteria utilize the citrate and convert the ammonium phosphate into ammonia and ammonium hydroxide.

86. Carotene is a yellow or orange pigment that serves as an accessory to chlorophyll during

The intention of this experiment is to determine the effects of pH on the rate of photosynthesis in living leaves. Photosynthesis is a process by which plants convert light energy captured from the sun into chemical energy which they use to perform various plant functions. During the photosynthesis process, light, carbon dioxide, and water react to produce products: sugar and oxygen. The equation for photosynthesis is:

It was seen that in comparison to low and high nutrients, there was a significant difference in growth rate when using a high nutrient sample. When comparing high/low light using a low nutrient sample, there was no significant difference between the data (Table 4). This may mean that light was not a major contributing factor when in a low nutrient environment. When looking at effects of nutrients, we ran comparisons with the same light intensity. We saw that there was no significant difference between low and high nutrients in neither low or high light (Table 5). This does not fully support our hypothesis because the comparison of highlight/low nutrient v. high light/ high nutrients

Carotenoids possess a significant amount of nutritional value. Therefore, it can be profitable to find the most effective procedure for extracting carotenoids. The purpose of this experiment was to extract carotenoids from green vegetables through gentle saponification. The experimenters sort through information provided by literature articles about saponification. The procedure for this experiment included carotenoid extraction, isolation, and quantification. Saponification also acts to filter out unwanted chlorophylls in the green vegetables. The chlorophylls skew the results of the experiment by increasing the turbidity of the test tubes. A spectrophotometer is used to measure the absorbance readings and directly correlate the absorbance

The enzyme catechol oxidase, extracted from masticated potato (Solanum tuberosum) lowers activation energy, as it is a catalyst. This enzyme can react with catechol to produce benzoquinone and water. Catechol oxidase is tested against a multitude of phosphate buffers, acidic, neutral and basic pH values, and chilled temperatures to hot temperatures. The purposes of these testes were to determine the optimal temperature and pHs at which catechol oxidase performs at. The method to measure results was the usage of a spectrophotometer (Vernier Spectrouis Plus). The spectrophotometer measures the absorbance levels of the pigment excreted when catechol oxidase undergoes a reaction. The high the absorbance, the more products produced and vise versa. The highest absorbance for the catechol oxidase submitted to different temperatures measured an average 0.6018 nm, when at 20 C. The highest absorbance for the catechol oxidase submitted to different pH values measured two averages of 0.658 at pH 6 and 0.6464 at pH 7. The conclusion taken from the available data explains that the optimal pH for catechol oxidase was between pH 6 and 7 and the optimal temperature was at room temperature at 20C.

Algae is considered to be one of the stable, traditional food for people in Mexico (Spirulina platensis) and for people in Chad (Spirulina maxima) (Kuhad et al. 1997). Spirulina is one of the cheapest sources of protein and essential vitamins(Babu and Rajasekaran 1991). It is also rich in ß-carotene and dietary gamma- linolenic acid (GLA) (Chronakis and Madsen 2011). Spirulina is declared as a GRAS (Generally Recognized as Safe) by the FDA (Food and Drug Administration) (FDA, 2003). Spirulina is produced extensively around different parts of the world (3000 tons/year) and used in food and animal feed(Gouveia et al. 2008).

The term ‘’Carbohydrates’’ is defined as the carbon compounds which contains hydroxyl groups in large quantities (King, 2013). Carbohydrates are those organic compounds which have the empirical formula CnH2nOn, or (CH2O)n (Fromm 1997). Carbohydrates are one of the four types of organic compounds in living cells that are produces during photosynthesis and are the important source of energy in both plants and animals (Ahmed 2014). They are built from monosaccharides. These monosacchradies are the small molecules that consists of three to nine carbon atoms and differ in size and in the stereochemical configuration at one or more carbon centers. The simplest carbohydrates also contain either an aldehyde or ketone compounds (freeman 2002). Carbohydrates are classified as simple and complex carbohydrates. The classification of carbohtdydrates is based on the chemical structure of the food and how fast it absorbs. Simple carbohydrates contains single or double sugars in their chemical structure whereas complex carbohydrates consists of three or more sugars (ahmed 2014). There are four major types of carbohydrates depending on their chemical composition.