The Analysis of Spinach Pigmentation During Photosynthesis (biology Lab Report)

The spinach plants were hole punched, mixed with 0.2% NaOH and dish detergent, placed in a syringe with the solution to have the oxygen

Photosynthesis occurs each time the sun’s light reaches the lives of a plant. The chemical ingrediants for photosynthesis are carbon dioxide (CO2), a gas that passes from the air into a plant via tiny pores, and water (H20), which absorbed from the soil by the plant’s roots. Inside leaf cells, tiny structures called chloroplasts use light energy to rearrange the atoms of the ingrediants to produce sugars, most importantly glucose (C6H12O6) and other organic molecules. Chlorophyll gives the plant its green color (Simon, 02/2012, pp. 92-93). Chemical reactions transfers the sun’s light energy into the chemical bonds that hold energy-carrying molecules. The most common are

Photosynthesis is the conversion of light energy to chemical energy into sugars. It is the process in plants that uses carbon dioxide, water, and sunlight from its surroundings and releases oxygen as a byproduct (6H2O+6CO2+light energy -> C6H12O6+6O2). Photosynthesis is required for plants because they are autotrophs, organisms that make their own food. Plants require a specific environment that is ideal to them to be able to carry out the process. Environmental conditions can either increase or decrease the rate of photosynthesis. Things like colors of light, pH, and temperature can all affect the rate of photosynthesis in plants.

Photosynthesis is the process in which plants consumed inorganic materials like solar light, carbon dioxide and water and converted it to an organic molecule like sugar and an inorganic gas like oxygen. Light is one of the major elements influencing the rate of photosynthesis; direct light concentration affects the noncyclic pathway (light

In the beginning of this experiment, our TA added water, salt, and 75/25 hexane/acetone to spinach leaves to a blender and blended the mixture to assume equal amounts for each group and to avoid erros if each student had to do the blending. The addition of the water to the mixture allowed the it to separate into a distinct organic layer after being run in a centrifuge, which was available to be collected at the top of the centrifuge. Salt reduces solubility, which will force the organic parts of the mixture (the desired pigments for example) to separate into the organic layer at the top. Lastly, 75/25 hexane/acetone is added because this is a moderately polar solvent and will useful for both the non-polar and polar pigments present within the spinach leaves. A mixed solution of hexanes and acetone must be used because acetone is very polar, while hexane in very non-polar, and the spinach leaves contain both non-polar and polar pigments in them that are important in the extraction and for further analysis. The mixture was placed in the centrifuge so the solids in the mixture (mostly cellulose) could be separated from the liquids into separate distinct layers for further extraction and testing. In the tube, the organic substances separated into the top layer, whereas the water layer remains at the bottom of the tube below the solid layer made up of mainly cellulose.

5ml pipettes graduated in 0.1 ml units with suction devices or automatic pipetters and tips

Photosynthesis can be defined as the process that living organisms such as plants use to translate the sun’s energy into chemical energy. Once the plant has translated the sun’s energy into chemical energy it then releases that energy and makes it readily available to other living things (Vermaas, 2007).

The first part of the experiment dealt with breaking down the spinach leaves in a mortar and pestle. Acetone was added to this to help with the breakdown of the spinach leaves. Once the spinach leaves were broke down enough that you could see the particles

After wearing the gloves we obtained a chromatography vial from professor and label it with my and my peer initials. We dried up the chromatography vial in fume hood and added 1 ml of chromatography solvent to the vial. Then we took a chromatography strip and measure it 1.5 cm with ruler from one end of the strip and drew a line with pencil we cut two small pieces below the pencil line to form a pointed end. We applied spinach on the strip using quarter to rub the spinach leaf on the line that we drew on the strip and put it into the chromatography vial and placed that in fume hood. We observed as the solvent was moving up the chromatography strip by capillary action. When the solvent was reached approximately 1 cm from the top of the strip then we removed the cap from the vial. We took out the strip from the vial using forceps and marked up the location of the solvent front because it evaporates quickly. We measure out the distance as well as the pigment in order to find out the rf value. Moreover we compared rf values to the one in reference list in order to identify the

Photosynthesis is a vital process that autotrophs use to transfer light energy into chemical energy. Photosynthesis ultimately produces O2 and glucose. It, like many other biological processes, can be affected by environmental variables. The variable that we altered in the following experiment are intensity, light wavelengths, and pigment types. In order to do this, we conducted three experiments. In the first experiment, we examined the effect of light intensity by placing vials with chloroplasts with DPIP at different light distances in which the results varied. Initially, 30cm away was the most effective for photosynthesis. Then 24cm appeared to be the most effective. Followed by 49cm at minutes 25 and 30. In the second experiment, we

Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special way of using the ATP they produce. Photosynthesis is a process where plants harness the sunlight they receive and they produce carbohydrates, as well as oxygen for living things and other plants. Now the sunlight ultimately powers the process of

     Photosynthesis is a reproductive system that occurs in plants. The main components required for photosynthesizing are sunlight, energy, water and mineral from soil, and carbon dioxide from the air. Once these components are combined they

Photosynthesis is the process that plants use to convert light energy from the sun into chemical energy for other organisms’ activities. The two stages responsible for this process are Photochemical reactions (Light reactions) and Biochemical reactions (Calvin cycle). The light reactions consist of two enzymatic protein complexes, Photosystem II and Photosystem I, in the thylakoid membrane of a chloroplasts that use light to reduce molecules to power the electron transport chain. Photosystem II contains a chlorophyll with an optimum wavelength of 680 nanometers that uses the light energy absorbed to excite electrons from the chlorophyll to a higher energy level while using some of the energy absorbed to pump hydrogen ions into the thylakoid membrane (Khan Academy). As water is being oxidized, the electrons stripped are being used to power the electron transport chain, transferring electrons from Photosystem II down to Photosystem I while releasing a biproduct of oxygen. The electrons in Photosystem I are also excited by light to a higher energy state reducing NADP to NADPH. The hydrogen ions that have been pumped into the thylakoid membrane are used for ATP synthase.

Photosynthesis is a very complicated process. It is not as simple as plants need a little sunlight, water, and carbon dioxide, and viola oxygen is produced. There are many steps and processes that occur during photosynthesis which make it very complicated. Now the actual word photosynthesis in Greek means photo- “light”, and –synthesis “putting together”. This is the overall basic foundation that photosynthesis stands behind. Photosynthesis can only happen in plants and some algae, due to them having an organelle called chloroplast. Chloroplast has a pigment, which is called chlorophyll. Chlorophyll is a light absorbing pigment, which allows the plant to control solar energy and use it to distribute energy and food for the plant itself. Chloroplasts are usually located in the green tissue in the interior of the leaf called the mesophyll. A usual cell has around thirty to forty chloroplast. In the inner compartment there is a thick fluid called the stroma, with a system of interconnected membranous

Photosynthesis is the conversion of unusable light energy to usable chemical energy inside chlorophyll. This energy is stored in sugar. The chemical equation for photosynthesis is 6CO2 + 6H2O ------> C6H12O6 + 6O2. Photosynthesis supports almost every organism in some way. The sugar that chemical energy is stored in can be transformed into ATP, the fuel that powers the majority of living things, through cellular respiration. (Farabee, 2007)