The Analysis of Spinach Pigmentation During Photosynthesis (biology Lab Report)

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The Analysis of Spinach Pigmentation<p>
During Photosynthesis <p>
University of Alabama<p>
I. Introduction:<p> &quot;Photosynthesis is the conversion of light energy to chemical energy that is stored in glucose or other organic compounds; it occurs in plants, algae, and certain prokaryotes&quot; (Campbell, G-16). The formula below is photosynthesis and &quot;the ingredients necessary to make a plant grow&quot; (KoK, 30).<p>
6CO_2 + 6H_2_0,,&#179; C_6_H_12_O_6_ + 6CO_2<p>
Photosynthesis occurs within chloroplasts which are in leaf cells. It takes carbon dioxide and water &quot;within the chloroplast, sunlight powers the conversion of these ingredients to a sugar called glucose and oxygen&quot; (Campbell, 37).<p> Pigments are substance
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Materials and Methods<p> To perform the first part of the lab it required: spinach leaves, a crucible, pipette, acetone, a beaker and chromatography paper. The chromatography paper was rolled up into a cylinder and stapled to hold it in place. Then a pencil was used to draw a line about 1 cm from the bottom of the paper. <p> Meanwhile, the spinach leaves were being crushed in the crucible into a more liquefied state. Once the spinach leaves were done, a pipette was used to place the spinach extract along the ling drawn around the cylinder. This step was repeated six time, because the darker the line the better the results of the chromatographic separation. After each application of the spinach extract, it was allowed to dry for at least one minute.<p>
Once the application of the spinach extract was finished, acetone, the solvent, was poured into the beaker. Then the cylinder was placed into the beaker to absorb the solvent with a jar top placed on top of it to hold it in place. The chromatography cylinder stayed in the beaker until the pigments in the spinach extract had been separated into different colors and was about 4-5 cm from the top of the paper. After this, the chromatography cylinder was removed to dry for the different pigments could be observed.<p> To perform the second half of the lab it required: a spectrophotometer,

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