The Anti Bacterial Properties Of Herbal Remedies

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Investigation of the anti-bacterial properties of the herbal remedies, tea tree oil and eucalyptus oil

Viktoria Budafai – G00269730
Group J

Biology 1.1 Laboratory Project
Brian Moran

Introduction 3
Hypothesis and aims 3
General introduction to herbal remedies 3
Tea tree oil 3
Eucalyptus oil 3
Penicillin G. 3
Gentamicin 3
Trigene 3
Sterile water 3
Escherichia Coli 3
Methods 4
Choosing reagents 4
Preparation of agar plates 4
Sterilisation of equipment 4
Transfer of E. coli. culture onto agar plates 4
Disk preparation and placement 4
Incubation 5
Results 5
Tea tree oil 5
Eucalyptus oil 5
Penicillin G. 5
Gentamicin 5
Trigene 5
Sterile water 5
Conclusion 7
Appendices 7
Bibliography 8

Hypothesis and aims
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EO is frequently used to treat viral infections such as influenza and the common cold. EO is also regarded to have antimicrobial activity against pathogenic bacteria, therefore it is also used to treat wounds to prevent infection.
Penicillin G.
Penicillin G. is also known as Benzylpenicillin. It is used to treat a variety of bacterial infections. Usually Penicillin G is obtained from microbial sources such as Escherichia coli (Cole, 1969). It works on most Gram-positive bacteria, and some Gram-negative bacteria.
Gentamicin is also an antibiotic, like Penicillin, and is used to treat a number of bacterial infections. Unlike Penicillin, Gentamicin is more effective on Gram-negative bacteria, and only works on a limited number of Gram-positive bacteria. It was first used in 1963 as the first clinically useful broad-spectrum antibiotic. (Edson RS, Terrel CL, 1999).
Trigene is a disinfectant and was used in the experiment as a positive control.
Sterile water
Sterile water was used in the experiment as a negative control.
Escherichia Coli
Escherichia coli (E. coli) is a pathogenic, Gram-negative bacteria, and was used in the experiment to determine the antimicrobial activity of TTO and EO.

Choosing reagents
Before the preparation for the experiment could be started, reagents needed to be picked. In the case of this experiment, the reagents were chosen to be TTO and EO.
Preparation of agar plates
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