The acronym, CRISPR, stands for “Clustered Regularly Interspaced Short Palindromic Repeats”. Also, Cas stands for “CRISPR associated protein.” Jensen R, et al (2002) carried out a study, and they found out that Cas genes tend to be found invariably in close proximity to CRISPR loci. They concluded that this indicates “a functional relationship” between the loci of CRISPR, and Cas proteins. In a recent article, based on a study performed by Westra, R.E, et al (2014), it is implied that the CRISPR-Cas system is paramount in prokaryotic “adaptive immunity” and in “the regulation of gene expression.” The catalytic duty of the CRISPR-Cas complex is performed by the Cas proteins. Memory of genetic encounters and activities is the responsibility…show more content… They are: Cas one, two, three, four, five, seven and eight. The gene loci which produce Cas eight, seven, three and four (FACI_IFERC00001G1217, FACI_IFERC00001G1218, FACI_IFERC00001G1219, FACI_IFERC00001G1220, FACI_IFERC00001G1221 respectively) belong to the same operon (Operon_0608). The other two Cas proteins (Cas 1 and Cas 2), which have loci FACI_IFERC00001G1223 and FACI_IFERC00001G1224 respectively, are part of Operon_0609.
For the sake of discussing the protein product formed by each locus, it is inherent that the terms hypothetical protein and putative protein be defined. A protein’s existence can be predicted. However, there may be no evidence, experimentally, to prove its expression, in vivo. Such a protein is called a hypothetical protein. Hypothetical proteins have less similarity with known proteins that have been annotated. On the contrary, putative proteins are more similar to characterized proteins in their levels conserved amino acid residues. Both FACI_IFERC00001G1223 and FACI_IFERC00001G1220 have protein products that are hypothetical. It must be added that, initially a search of the entire Ferroplasma genome was done. The data from this ‘whole-genome research’ was very helpful in assisting with the formulation of hypotheses pertaining to protein products, which should be observed for the various loci during this Genomic annotation assessment. As stated earlier, the proteins which were encoded for by the loci associated