The Cell Number On The Nanotube With 30 Nm Diameter

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Xianglong et al. found similar results (2) in their paper. The cell number on the Nanotube with 30 nm diameter was significantly higher than the one without the nanotubes. For each incubation time, the surface with the absence of nanotubes had the least cell adhesion. Even with respect to the filopodia the nanotubes showed similar behavior. On the surface withy nanotubes of 30 nm diameter, the actin of the cells was organized along the spreading direction and had formed many filopodia. Interestingly, most of the cells on the surface of 80 nm diameter group maintained a round or oval shape, but the cells also stretched out many filopodia. Cells on the three nanotextured surface had stretched out many filopodia and some lamellipodia. In particular, the cellular cytoskeleton of cells on the 80 nm surface achieved a more homogeneous and extensive arrangement compared with those of the other three groups. The shapes of cells grown on the SLA + 30 nm and SLA + 80 nm surfaces were clearly different. It was observed that those grown on the SLA + 80 nm surface were the most irregularly shaped, while those grown on the SLA + 30 nm surfaces had relatively regular shapes.
Gene expression on the titanium surfaces was quantified using RT-PCR.ALP expression level was detected higher at week 1 and was subsequently greatly decreased at week 2. The expression level of ALP in cells grown on the 80 nm surface was more than that in cells grown on other surfaces at all-time points. The lowest
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