The Delivery Method, And Primary Scientific Literature

1711 WordsNov 2, 20157 Pages
The ability to transform cell cultures and incorporate foreign DNA into other tissues is a key component to studying gene function which allows research to further in biotechnology (Gao and Nielson, 2013). The most commonly used biotechnology methods for integrating foreign genes into plant cells are Agrobacterium-mediated transformation and Biolistic delivery mediated transformation (Gao and Nielson, 2013). For the purpose of this technical review paper, the focus will be on the biolistic delivery method, and primary scientific literature that incorporates this technique in their studies. The biolistic delivery method uses microprojectiles, typically gold or tungsten that are coated with DNA or other substances, which are then expelled at high velocities by built up helium pressure in order to penetrate cell walls and membranes (Sanford, 1990). This technology is very quick and easy to use, and its versatility makes it an excellent method to use in the field because it can be applied to many different targets including, cell cultures, tissues, organs, plants, animals, and bacteria (Bio-Rad, 2013). This review paper is going to be examining the applications of this method in regards to plant systems, animal systems and other biological systems. The biolistic delivery mediated transformation as mentioned earlier, is one of the most commonly used techniques when integrating foreign genes into plant materials (Gao and Nielson, 2013). In this first study executed by Fadeev and
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