The Effect Of Bacterial Growth On Space Flight

Aim: To plan and carry out an investigation to show the relationship between volume/Cm3, surface area, and diffusion using agar cubes measured in time/s; and to demonstrate, using diffusion, why the size of cells is limited, keeping the room temperature and pressure constant.

The purpose of this report is to analyse the growth of the bacteria known as Citrobacter Freundii as well as distinguishing what antibiotics effect its growth. This will be done so by answering the following question after completing its associated experiments. This question includes: what antibiotics are most effective in denaturing the bacteria? It has been predicted that chloramphenicol will be the most effective due to the fact that its medical uses are treating meningitis which is an infection caused by Citrobacter freundii. After conducting the experiments it was found that the chloramphenicol antibiotic was the most effective in denaturing the bacteria, although streptomycin was also affective. However, none of the other antibiotics were able to halt the growth of the bacteria.

Part two of The Hot Zone is important to the rest of the text because a lot was revealed in part two. In part two the virus was almost given the wrong diagnosis of SHF, simian hemorrhagic fever. It was later discovered by Geisbert when he decided to reexamine the bacteria that their diagnosis was wrong and that they were dealing with a hot agent. Geisbert and Jharling sniffed the virus that they found out was ebola and could be at risk. This adds suspense and causes the reader to worry about the safety of both Geisbert and Jharling and also people around the world if the virus were to spread. And of course, once they find out that the virus is ebola they all come together to find a solution so that the world can remain danger free. They are

The purpose of the bacterial unknown independent study experiment completed throughout the course of this lab was to determine the identity of an unknown bacterial species. The unknown bacteria sample was chosen from numerous samples provided by the instructor. The starting unknown sample, unknown #15 was a mixed bacterial culture and a broad approach taken to identify the sample. Various biochemical tests were completed to identify the bacterial species along with the use of databases such as Gideon and Bergey’s to compare the test results of known bacteria to the results of the unknown sample. Information was gathered from the other sources and databases and phenotypic testing completed and the results compared to the database results. Aseptic

As shown in the figure above, it is evident that V.natriegens grew faster when the Brain Heart Infusion (BHI) broth contained 250mM NaCl. The # of bacterial cells at each time point was measured following the equation given in the “How to generate a bacterial growth curve” supplemental material posted on D2L. (2) The data was then recorded in the table listed above. A growth curve graph was constructed using the data above which illustrated the differences between each of the different BHI mixtures. The graph was then used to determine the generation time of V.natriegens for each different environmental condition. In order to calculate the generation time (g) the mean growth rate (k) must be calculated. The formula to do this is posted in the supplemental material “How to generate a bacterial growth curve” on D2L. The k value calculated for each condition goes as follows:

Microorganisms need energy and that is generated through these metabolic activities (Jurtshuk, 1996). Through this experiment, it is shows that all the bacteria experienced different metabolic activities in different mediums.

After gaining some knowledge about bacteria, we were giving an investigating bacteria growth lab to do. Our objective was to observe the conditions required for bacteria to grow and to test the effectiveness of substances that may be antibacterial, disinfecting, and or sanitizing. My group and I began our procedure by gathering all the bacteria by swabbing our necks and mouths. After this, we inoculated the culture by rubbing the bacteria on the agar, a nutrient rich gel made from sea kelp, on the bottom side of the container where we grow bacteria, the Petridish. We hoped for the results to come back with little or even no colonies and an immense zone of inhibition around the tiny circle cut out of filter paper covered in toothpaste, Neosporin, and Chlorhexidine Gluconate 4% Solution.

Antibiotics are important for the treatment of bacterial infections, and it is therefore important to know how different antibiotic works in different contexts. Different kinds of bacteria are affected to different degrees by different antibiotics, and it may therefore be useful to know which antibiotic that attacks the bacteria. The purpose of this report is to analyze how antibiotics including Chloramphenicol and Ampicillin affect the growth rate of bacteria Escherichia coli and Bacillus subtilis. Which antibiotic is the most effective or least effective in inhibiting the growth rate of these two bacteria respectively?

From 1961 to 1972, the government-funded organization NASA ran the Apollo program, which attempted to land humans on the Moon and bring them back to Earth safely. Of the program’s 16 successful flights, five flights landed men on the Moon and a total of twelve men walked on the Moon. Samples taken from the Moon’s surface paved the way for enormous discoveries and increased understanding about space, natural elements and our Moon’s composition. However, one aspect of discovery that the Apollo missions enabled was the discovery of the human body’s reaction to space and weightless environments.

The purpose of this lab was to observe the locations of bacteria growing in our school. I hypothesized that there would be more bacteria growing in areas with more exposure to students. My hypothesis was refuted. By the end of the growing period, the quadrant with the swab from the arm of the union bench had one colony, the quadrant with the swab from the girls’ locker room floor had 225 colonies, the quadrant with the swab from a soap dispenser had no colonies, and the quadrant with the swab from inside a toilet bowl had six colonies. This means that exposure to students was not the factor in determining the amount of bacterial growth, because the arm of the union bench has more exposure to students than the inside of a toilet bowl, and because

In the United States, the fourth most leading cause of death are hospital-acquired infections. Furthermore, it is estimated that greater than 65 percent of all bacterial infections are associated with biofilms. A greater understanding of biofilms is essential if we are to find effective methods to combat their formation in order to promote public health. Unfortunately, with bacteria in space behaving widely different than on Earth, this can cause a huge problem when it comes to health in space. First of all, biofilms could contaminate and bio-deteriorate the space habitats, the health of the crew, and the function of waste recycling or food production systems in extremely different ways then handled before on Earth. All of these issues would

For this experiment, a non-virulent strain of Escherichia coli was used to illustrate differences in growth rates for different temperatures. The culture was created by placing 35.0 ml of L-Broth into a 125 ml flask and adding 0.025 ml of an overnight bacteria culture. This culture mixture was divided and placed into 3 shaking incubators that were kept at 25˚C, 30˚C, or 37˚C (Timmons 2004).

This experiment is about bacterial growth. We will demonstrate a bacterial growth curve using a closed system. Bacterial growth usually takes up to 12-24 hours to get an accurate result so we will be monitoring this growth between two classes. We also used different methods to determine bacterial growth as well as a few different calculations. One way of receiving data is by using a spectrophotometer where we will record the absorption at a given time to create the bacterial growth curve. We also used the plate count method after performing a serial dilution to calculate the actual cell density at different times given. By using this method we can count the population number of the same given and see the maximum cell density

Bacteria grows by binary fission. The aim of this experiment is to follow the growth of Serratia marcescens in nutrient broth at 37oCby recording the changes in turbidity (cloudiness) by measuring the absorbance of visible light (600 nm) and also to prove that there is an increase in the cell number and not just in mass during the growth. In the experiment we measure the full growth curve of Serratia marcescens by measuring the absorbance at 600nm at every 10 mins. I also determined the viable count at the start and the end of the exponential phase of growth. Using the growth curve I calculated the growth curve and it was 1.2. Using this I found the doubling time which was 34s.

Being able to control bacterial growth is something that is important in our everyday lives. As shown in the previous labs, bacteria can grow and create colonies extremely quickly especially in the right environments. By acknowledging this, it is then important to get an understanding of how bacterial growth can be controlled by humans. To control microorganisms it means to inhibit their growth (static) and or kill them (cidal) (Kenneth Todar, 2015); therefore since focusing on bacteria the terms bactericidal and bacteriostatic are both extremely important for this lab. One broad method we will use to control bacterial growth is heat. The amount of heat needed to control bacterial growth is different for different species of bacteria (Kenneth Todar, 2015). Bacteria can also respond differently depending if moist heating method such as an autoclave with steam is used, or a dry heating method such as inoculating a loop over a fire is used (Kenneth Todar, 2015). UV works by damaging the cells DNA, without proper DNA, the cells will die and the object